... that involves the directed migration of lymphatic endothelial cells (LECs) to form lymphatic vessels. The molecular mechanisms that underpin lymphatic vessel patterning are not fully elucidated and, to date, no global regulator of lymphatic vessel guidance is known. In this study, we identify the transmembrane...

... microscope systems. We demonstrate the utility of this new system by nearly day-long observation of neutrophil recruitment to a wound area in living double-transgenic adult casper zebrafish with fluorescently labeled neutrophils and lymphatic vessels, as well as intubating and imaging the same fish...

... malformations. It is still unknown whether meningeal lymphatic networks, which are patterned alongside the venous sinuses, are also affected. We now show that the growth and expansion of meningeal lymphatics are perturbed in Twist1 craniosynostosis models. Changes to the local meningeal environment, including...

...Mathias Francois; Anna Oszmiana; Natasha L. Harvey ABSTRACT The lymphatic vasculature is an integral component of the cardiovascular system. It is essential to maintain tissue fluid homeostasis, direct immune cell trafficking and absorb dietary lipids from the digestive tract. Major advances in our...

... the formation of intersegmental arteries by primary angiogenesis and Vegfc is essential for secondary angiogenesis, giving rise to veins and lymphatics. Vegfd has been largely thought of as dispensable for vascular development in vertebrates. Here, we generated a zebrafish vegfd mutant by genome editing. vegfd...

...Masahiro Shin; Ira Male; Timothy J. Beane; Jacques A. Villefranc; Fatma O. Kok; Lihua J. Zhu; Nathan D. Lawson Vascular endothelial growth factor C (Vegfc) activates its receptor, Flt4, to induce lymphatic development. However, the signals that act downstream of Flt4 in this context in vivo remain...

...Jonathan Semo; Julian Nicenboim; Karina Yaniv The lymphatic system is a blind-ended network of vessels that plays important roles in mediating tissue fluid homeostasis, intestinal lipid absorption and the immune response. A profound understanding of the development of lymphatic vessels, as well...

...Jonathan W. Astin; Michael J. L. Haggerty; Kazuhide S. Okuda; Ludovic Le Guen; June P. Misa; Alisha Tromp; Benjamin M. Hogan; Kathryn E. Crosier; Philip S. Crosier Lymphangiogenesis is a dynamic process that involves the sprouting of lymphatic endothelial cells (LECs) from veins to form lymphatic...

...Jacques A. Villefranc; Stefania Nicoli; Katie Bentley; Michael Jeltsch; Georgia Zarkada; John C. Moore; Holger Gerhardt; Kari Alitalo; Nathan D. Lawson Vascular endothelial growth factor C (Vegfc) is a secreted protein that guides lymphatic development in vertebrate embryos. However, its role...

...Kazuhide S. Okuda; Jonathan W. Astin; June P. Misa; Maria V. Flores; Kathryn E. Crosier; Philip S. Crosier We have generated novel transgenic lines that brightly mark the lymphatic system of zebrafish using the lyve1 promoter. Facilitated by these new transgenic lines, we generated a map...

... A. , Kawakami K. , Duckers H. J. , Schulte-Merker S. ( 2010 ). Arteries provide essential guidance cues for lymphatic endothelial cells in the zebrafish trunk . Development 137 , 2653 - 2657 . Carmeliet P. , Tessier-Lavigne M. ( 2005 ). Common mechanisms of nerve and blood...

... expression in Gata2 -GFP knock-in embryos. Using founder transgenic analyses, we identified a Gata2 endothelium enhancer in the fourth intron and found that Gata2 regulation by this enhancer is restricted to the endocardial, lymphatic and vascular endothelium. Whereas disruption of three ETS-binding motifs...