...-term self-renewing (e.g. stem cell) population. Newly developed generalized birth-death modeling of long-term lineage tracing data shows that both TA progenitors and stem cells display neutral competition, but only the stem cells display neutral drift. The quantitative evolution of a nascent TA cell...

... in response to laser-assisted osteoblast ablation, albeit not frequently. In addition to proliferation, stretching of cellular processes of pre-existing osterix + osteoblasts towards the lesion site and lineage tracing of the same cells confirm contribution of committed osteoblasts. A potential alternative...

... hematopoietic stem cells (HSCs), many TLCs are of fetal origin and poorly generated from adult HSCs. Here, we sought to further understand murine TLC development and the roles of Flk2 and IL7Rα, two cytokine receptors with known function in traditional lymphopoiesis. Using Flk2- and Il7r-Cre lineage tracing, we...

... and applications to lineage tracing in live-imaging data and outline software methodologies that can be applied to various cell-tracking challenges. Beyond the introduction and advancement of new microscopes capable of imaging large and sensitive specimens (of which there has been an explosion in recent years...

... systems showing replacement of mechanosensory cells can provide mechanistic insights into developing new regenerative therapies. Here, we developed lineage tracing systems to reveal the generation of mechanosensory neurons in the Johnston's organ (JO) of intact adult Drosophila , which are the functional...

... in the ocular surface epithelium during homeostasis and injury repair, and to molecularly characterize each population. The characteristics of slow-cycling cells in the ocular surface epithelium have been difficult to ascertain given a lack of definitive molecular markers and lineage tracing mouse models...

... and migration routes. By marking progenitor cells, one can observe their subsequent locations and the cell types into which they differentiate. Here, we review major discoveries in neural crest lineage tracing from a historical perspective. We discuss how advancing technologies have refined lineage-tracing...

..., that are maintained in a lineage-restricted manner during homeostasis or after mild injuries. Glandular regeneration following a near complete loss of secretory cells, however, may involve cellular plasticity, although the mechanism and extent of such plasticity remain unclear. Here, by combining lineage-tracing...

.../progenitor cells. However, the precise stem cell niche organization, the intermediate steps and the endogenous regulators of postnatal hypothalamic neurogenesis remain elusive. Quantitative lineage-tracing in vivo revealed that conditional deletion of fibroblast growth factor 10 (Fgf10) from Fgf10-expressing...

... by equal base composition, 5′ G sequence, minimal likelihood of residing in the functional genome, minimal off targets and ease of amplification. We validate multiple high-quality endogenous CRISPR/Cas9 arrays , demonstrating their utility for lineage tracing. Our pragmatically scalable technique thus can...

.... With developmental plasticity, two lineages (larval EG and gcm -positive lineages) ensure the robust development of adult neuropil-glia architecture. Compensation Astrocyte-like glia Ensheathing glia Heartless Prospero Lineage tracing Metamorphosis Ministry of Education, Culture, Sports...

... allele to lineage trace AMFs, we demonstrate that most AMFs are developmentally cleared during alveologenesis. Lung mesenchyme also contains other poorly described cell types, including alveolar lipofibroblasts (ALF). We show that Gli1:CreERT2 marks both AMFs as well as ALFs, and lineage tracing shows...

... are self-sustained in adult mice by unipotent lineage-restricted SCs. The spatiotemporal regulation of SC fate and the switch from multipotency to unipotency remain poorly characterised. Here, by combining lineage tracing, whole-tissue imaging, clonal analysis and proliferation kinetics, we uncover...

... lineage tracing of cells with an expression history of IL7Rα, a marker exclusively associated with the lymphoid lineage in adult hematopoiesis. Surprisingly, we found that Il7r-Cre labeled fetal-derived, adult trMacs. Labeling was almost complete in some tissues and partial in others. The putative...

... questions that can be addressed using single cell dynamic lineage tracing. * Authors for correspondence ( [email protected] ; [email protected] ) Funding A.M. was supported by the National Human Genome Research Institute of the National Institutes of Health under grant number...

... and electron microscopic study . Z. Anat. Entwicklungsgesch.   135 , 156 - 184 . 10.1007/BF00521108 Kopinke , D. , Brailsford , M. , Shea , J. E. , Leavitt , R. , Scaife , C. L. and Murtaugh , L. C. ( 2011 ). Lineage tracing reveals the dynamic contribution of Hes1+ cells...

.... ( 2013 ). Adipose tissue heterogeneity: implication of depot differences in adipose tissue for obesity complications . Mol. Asp. Med.   34 , 1 - 11 . 10.1016/j.mam.2012.10.001 Lepper , C. and Fan , C.-M. ( 2010 ). Inducible lineage tracing of Pax7-descendant cells reveals embryonic...

... under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/3.0 ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Summary: Neutral, low-density lineage tracing...

.... As development proceeds, these clusters expand and further contribute to the growing lymphatic system. Lineage tracing and analyses of Gata2 -deficient mice confirmed that these clusters are endothelial in origin. Furthermore, ectopic expression of Vegfc in the vasculature increased the number of PROX1-positive...

... of these cells is difficult to prove, however, as neither lineage tracing of human trophoblast nor live imaging are possible because of the optical density of the CCCs and the floating nature of the villi. We used a thymidine analogue pulse-chase strategy as a preliminary attempt to study the differentiation...