...Saya Furukawa; Sakiya Yamamoto; Ayaka Ohashi; Yoshihiro Morishita; Akira Satoh ABSTRACT Axolotl ( Ambystoma mexicanum ) limb regeneration begins with blastemas of various sizes, in contrast to the limb developmental process. Despite this size variation, normal limb morphology, consistent...

... in the proximal-distal axis of limbs. However, there is no direct genetic evidence that Hox genes are essential for limb development in non-mammalian tetrapods or for limb regeneration. Here, we produced single to quadruple Hox13 paralog mutants using the CRISPR/Cas9 system in newts ( Pleurodeles waltl ), which...

...Can Aztekin; Tom W. Hiscock; John Gurdon; Jerome Jullien; John Marioni; Benjamin David Simons ABSTRACT Absence of a specialized wound epidermis is hypothesized to block limb regeneration in higher vertebrates. However, the factors preventing its formation in regeneration-incompetent animals...

... BMP signaling is blocked, but ectopic injection of either human or axolotl protein did not rescue the defects. By administering LDN193189 treatments at different time points during regeneration, we show clearly that limb regeneration progresses in a proximal to distal fashion. This demonstrates...

...Stephanie L. Tsai; Clara Baselga-Garriga; Douglas A. Melton ABSTRACT Blastema formation, a hallmark of limb regeneration, requires proliferation and migration of progenitors to the amputation plane. Although blastema formation has been well described, the transcriptional programs that drive...

... of developing and regenerating teleost fins. * Author for correspondence (e-mail: [email protected] ) 28 7 1998 30 9 1998 © 1998 by Company of Biologists 1998 blastema Danio rerio developmental patterning hedgehog Patched Bmp limb regeneration...

... ectodermal cap (AEC) during limb regeneration. Expression is maximal at the late bud stage of regeneration, coincident with the transition from the early phase of nerve dependency to the later phase of nerve independence. Dlx-3 expression in the epidermis is rapidly downregulated by denervation during...

...David M. Gardiner; Bruce Blumberg; Yuriko Komine; Susan V. Bryant ABSTRACT Homeobox genes are important in the regulation of outgrowth and pattern formation during limb development. It is likely that homeobox genes play an equally important role during limb regeneration. We have isolated...

... Msx2 limb regeneration limb development mouse embryo digit tip The ability of limb cells to replace lost parts following injury (regeneration) is known to occur in adult digits of a variety of different mammals including humans ( Douglas, 1972 ; Holm and Zachariae, 1974 ; Illingsworth...

... , J. P. ( 1988 ). Position dependent expression of a homeobox gene transcript in relation to amphibian limb regeneration . EMBO J . 7 , 4275 – 4282 . 10.1002/j.1460-2075.1988.tb03325.x Simon , H.-G. and Tabin , C. J. ( 1993 ). Analysis of Hox-4.5 and Hox-3.6 expression...

... W1P 8BT, UK 26 October 1993 © 1994 by Company of Biologists 1994 biolistics wound epidermis retinoic acid receptors limb regeneration mucous metaplasia Epithelia are a classic target for retinoid action during development and in the adult, and the effects of both...

... isolated both the bek and KGFR variants of FGFR2. These two variants differ only in the second half of the last of their three Ig-like domains. The expression patterns of FGFR1 and FGFR2 during limb regeneration have been determined by in situ hybridization. During the preblastema stages of regeneration...

..., a consistent pattern emerges, which can be interpreted in terms of differential roles for the dif-ferent Hox clusters in determining regenerative limb morphology. 08 01 1993 © 1993 by Company of Biologists 1993 Hox-3.6 Hox-4.5 paralogues homeobox genes limb regeneration positional...

... and wound epidermis were strongly reactive, although no axial variation in expression was detected. 15 12 1992 © 1993 by Company of Biologists 1993 retinoic acid retinoic acid receptors limb regeneration newts Limb regeneration in urodele amphibians such as the newt...

... of retinoic acid, the level of expression in a distal blastema was not increased in comparison to controls. * Author for correspondence 19 10 1990 © 1991 by Company of Biologists 1991 homeobox limb regeneration positional information retinoic acid axis specification Limb...

...Hiroaki Onda; David J. Goldhamer; Roy A. Tassavat Several well-characterized extracellular matrix (ECM) components have been localized to the amphibian limb regenerate, but the identification and characterization of novel ECM molecules have received little attention. Here we describe, using mAb MT1...

...Clifford J. Tabin ABSTRACT Forelimbs and hindlimbs of tetrapods have different morphological patterns. One plausible explanation for the difference is that the cells that give rise to the limbs differentially express genes which control their pattern of development. Amphibian limb regeneration...

..., the two sets of data seem to show a similar stage-dependent rise and then decline in the availability of CRABP, implying a role for this molecule in limb regeneration (see below). In an effort to examine the role of CRABP in more detail, we have compared the ability of a range of retinoids...

... for the axolotl forelimb. We show that the complex forms autonomously in muscleless axolotl limb regenerates produced from a cuff of unirradiated dermis surrounding an irradiated limb stump, and persists for up to a year after amputation. The pattern of other connective tissue structures, including the skeleton...

... W1P 8BT. UK 11 04 1988 aneurogenic limb limb regeneration limb development growth control blastema monoclonal antibody Pleurodeles waltl The regeneration of limbs in adult urodele amphibians proceeds by the formation of blastemal (progenitor) cells at the amputation...