... outgrowth. Here we show that Tbx5 is expressed throughout the limb mesenchyme in progenitors of cartilage, tendon and muscle. Using a tamoxifeninducible Cre transgenic line, we map the time frame during which Tbx5 is required for limb development. We show that deletion of Tbx5 subsequent to limb initiation...

... and an additional leg-like limbs were induced, respectively. This additional limb formation was accompanied by the induction of both Wnt and Fgf genes These results highlight the pivotal roles of Tbx5 and Tbx4 during limb initiation, specification of forelimb/hindlimb and evolution of tetrapod limbs, placing Tbx...

[email protected] ) 28 02 1997 © 1997 by Company of Biologists 1997 FGF10 lateral plate mesoderm limb initiation FGF8 epithelial-mesenchymal interaction The chick limb stands as an ideal model system to elucidate the mechanisms that coordinate growth and patterning during...

...Maria A. Ros; Alric López-Martínez; B. Kay Simandl; Concepción Rodriguez; Juan Carlos Izpisúa Belmonte; Randall Dahn; John F. Fallon ABSTRACT We have analyzed the pattern of expression of several genes implicated in limb initiation and outgrowth using limbless chicken embryos. We demonstrate...

..., Fgf - 8 transcripts are detected in the intermediate mesoderm and subsequently in the prelimb field ectoderm prior to the formation of the apical ectodermal ridge, structures required for limb initiation and outgrowth, respectively. Later on, Fgf - 8 expression is restricted to the ridge cells...