... types to their temporal and spatial relationships. Human development Cardiac Deconvolution Single-nucleus RNA-sequencing Spatial transcriptomics Light-sheet microscopy Institut National de la Santé et de la Recherche Médicale http://dx.doi.org/10.13039/501100001677...

...: A versatile light-sheet imaging setup enabling simultaneous live imaging of multiple mouse embryos for 48 h, an approach that offers insight into the onset of oscillatory signaling dynamics and the segmentation clock. Light-sheet microscopy Notch signaling Segmentation clock Lunatic fringe Period...

... highlight how in toto light-sheet microscopy facilitates accurate reconstruction of brain and craniofacial structures within X. tropicalis embryos upon dyrk1a and six1 loss of function or treatment with retinoic acid inhibitors. These tools increase the sensitivity and throughput of evaluating developmental...

... in the next section. Fig. 3. Optimization of light-sheet microscopy for embryology: maximizing the resolution. (A) Schematic of light-sheet illumination and light collection (example of the ultramicroscope). (B) Importance of the numerical aperture (NA) for the light-sheet generation and its...

... with multispectral light-sheet microscopy, the validated protocol presented here provides a rapid, inexpensive and reliable method for acquiring accurate histological images that preserve three-dimensional structural relationships with single-cell resolution in whole early-stage chicken embryos and in the whole...