.... Summary: A versatile light-sheet imaging setup enabling simultaneous live imaging of multiple mouse embryos for 48 h, an approach that offers insight into the onset of oscillatory signaling dynamics and the segmentation clock. Light-sheet microscopy Notch signaling Segmentation clock Lunatic...

... highlight how in toto light-sheet microscopy facilitates accurate reconstruction of brain and craniofacial structures within X. tropicalis embryos upon dyrk1a and six1 loss of function or treatment with retinoic acid inhibitors. These tools increase the sensitivity and throughput of evaluating developmental...

... interactions, in large 3D volumes (around 1 cm 3 ). Therefore, innovations in large-scale light-sheet microscopy are needed to enable novel applications for tissue clearing and light-sheet imaging, which can be applied to developmental biology and bridge the resolution gap with thin-section histology. Higher...

... with multispectral light-sheet microscopy, the validated protocol presented here provides a rapid, inexpensive and reliable method for acquiring accurate histological images that preserve three-dimensional structural relationships with single-cell resolution in whole early-stage chicken embryos and in the whole...