... light so it is slightly lower than the critical angle. This results in an inclined light-sheet propagating into the sample, which reduces out-of-plane excitation ( Fig. 4 C). HILO is widely used for SMT in single cells grown on glass; however, contrast degrades quickly at deeper positions due...

...Ryan S. Udan; Victor G. Piazza; Chih-wei Hsu; Anna-Katerina Hadjantonakis; Mary E. Dickinson Single/selective-plane illumination, or light-sheet, systems offer several advantages over other fluorescence microscopy methods for live, 3D microscopy. These systems are valuable for studying embryonic...