... for live imaging, and that this protein can be used for quantitative applications, such as FRAP or laser ablation. Finally, we believe that this fluorescent protein, with the set of new possibilities it offers, constitutes an important tool for cell, developmental and mechano-biologists in their current...

... auxin transport . Nature 426 , 255 - 260 . 10.1038/nature02081 Reinhardt , D. , Frenz , M. , Mandel , T. and Kuhlemeier , C. ( 2005 ). Microsurgical and laser ablation analysis of leaf positioning and dorsoventral patterning in tomato . Development 132 , 15 - 26...

... is limited, additional complementary models would be useful. Here, we show that the ENS in zebrafish larvae at 10-15 days postfertilization is highly regenerative. After laser ablation, the number of enteric neurons recovered to ∼50% of the control by 10 days post-ablation (dpa). Using transgenic lines...

... density increased along the AP axis, thus relieving strain. We used laser ablation to isolate mesectoderm cells from the influence of other tissues. Uncoupling the mesectoderm from intercalating cells did not affect cell division orientation. Conversely, separating the mesectoderm from the anterior...

... contributes to this patterning. Phyllotaxis Patterning Meristem Laser ablation Auxin PIN1 Tomato Leaves and flowers are arranged in regular patterns around the stem, a phenomenon known as phyllotaxis ( Reinhardt, 2005 ; Kuhlemeier, 2007 ). Most frequent is spiral phyllotaxis, in which...

... play a central role in establishing proper fates along the entire DV axis in this animal; laser ablation of midline cells causes a failure to form neurogenic ectoderm and Ph-sim RNAi results in severely dorsalized embryos lacking both neurogenic ectoderm and the appendage-bearing lateral ectoderm...

...-existing primordia influence further phyllotaxis. Here, we repeat these experiments and compare them with the results of laser ablation of incipient primordia. Furthermore, we explore to what extent the different pre-existing primordia influence the size and position of new organs, and hence phyllotaxis...

... outgrowth of follower axons. Here, the dependencies between early and late outgrowing axons in the ventral cord were studied systematically with laser ablation experiments and a detailed analysis of mutants using multi-color GFP markers. Different classes of axon were affected to a different extent when...

... of the stem cells. We have undertaken a series of high-precision laser ablation and microsurgical tissue removal experiments to test the functions of different parts of the tomato meristem, and to reveal their interactions. Ablation of the central zone led to ectopic expression of the WUSCHEL gene...

...), and four multidendritic neurones (ddaA, ddaB, ddaC, ddaD). (B) Detail of the lateral cluster and dbd in a live specimen prior to laser ablation. (C) The same neurones as shown in B 12 hours after laser ablation of the four neurones ventral to dbd. One of the neurones is missing (*) and the other three...

... Primary motoneuron Muscle pioneer Single-cell transplants Mosaic analysis Laser ablation Genetic mapping Formation of appropriate connections between motoneurons and their target muscles is essential for animals to carry out their normal behaviors. En route to their targets, motor axons...

...Jeff Hardin It has long been thought that traction exerted by filopodia of secondary mesenchyme cells (SMCs) is a sufficient mechanism to account for elongation of the archenteron during sea urchin gastrulation. The filopodial traction hypothesis has been directly tested here by laser ablation...