... sequencing (WES) approach that allows rapid and cost-effective identification of mutations. We used our WES methodology to isolate four mutations that cause kidney cysts; we identified novel alleles in two ciliary genes as well as two novel mutants. The WES approach described here does not require...

... uninjected embryos showed kidney cyst and body curvature, none of the 47 injected embryos were phenotypic. Genotyping experiments on 44 injected embryos verified the presence of seven homozygous mutant embryos in the injected sample, suggesting that the injected mRNA can rescue sco hi459 mutants...

... Pronephros Kupffer's vesicle Ependymal cell Spinal canal Kidney cyst Hydrocephalus Left-right asymmetry Cilia and flagella have well-established roles as motile and sensory organelles in various species; however, their function in vertebrate organogenesis is only now coming to light...