...Hannah M. Wesselman; Ana L. Flores-Mireles; Aidan Bauer; Liming Pei; Rebecca A. Wingert ABSTRACT Cilia are essential for the ontogeny and function of many tissues, including the kidney. Here, we report that transcription factor ERRγ ortholog estrogen related receptor gamma a (Esrrγa) is essential...

...Anna-Carina Weiss; Eva Blank; Tobias Bohnenpoll; Marc-Jens Kleppa; Reginaldo Rivera-Reyes; Makoto Mark Taketo; Mark-Oliver Trowe; Andreas Kispert ABSTRACT The murine kidney and ureter develop in a regionalized fashion from the ureteric bud and its surrounding mesenchyme. Whereas the factors...

... single cell and bulk RNA-seq, quantitative immunofluorescent lineage/fate tracing, and genetically modified human induced pluripotent stem cells (iPSCs) to revisit this question in developing mouse kidneys and human kidney organoids. We confirmed that Notch signaling is needed for maturation of all...

...Elliot A. Perens; Jessyka T. Diaz; Agathe Quesnel; Amjad Askary; J. Gage Crump; Deborah Yelon ABSTRACT Transcriptional regulatory networks refine gene expression boundaries to define the dimensions of organ progenitor territories. Kidney progenitors originate within the intermediate mesoderm (IM...

... cardiovascular health throughout life. We show here that, despite its negative effects on kidney growth, genetic increase of GDNF prolongs the nephrogenic program beyond its normal cessation. Multi-stage mechanistic analysis revealed that excess GDNF maintains nephron progenitors and nephrogenesis through...

...Brooke E. Chambers; Eleanor G. Clark; Allison E. Gatz; Rebecca A. Wingert ABSTRACT A functional vertebrate kidney relies on structural units called nephrons, which are epithelial tubules with a sequence of segments each expressing a distinct repertoire of solute transporters. The transcriptiona`l...

... Wallingford ABSTRACT Laminin alpha 5 (LAMA5) is a member of a large family of proteins that trimerise and then polymerise to form a central component of all basement membranes. Consequently, the protein plays an instrumental role in shaping the normal development of the kidney, skin, neural tube, lung...

.... This fundamentally deepens our knowledge about the genetic control of kidney development. Kidney Nephron Segmentation Differentiation tfap2a tfap2b irx3b irx1a Zebrafish Vertebrate kidney ontogeny involves the reiterative formation and degradation of up to three structures from...

...Caramai N. Kamei; Thomas F. Gallegos; Yan Liu; Neil Hukriede; Iain A. Drummond ABSTRACT Zebrafish kidneys use resident kidney stem cells to replace damaged tubules with new nephrons: the filtration units of the kidney. What stimulates kidney progenitor cells to form new nephrons is not known. Here...

...Santhosh V. Kumar; Pei X. Er; Kynan T. Lawlor; Ali Motazedian; Michelle Scurr; Irene Ghobrial; Alexander N. Combes; Luke Zappia; Alicia Oshlack; Edouard G. Stanley; Melissa H. Little ABSTRACT Kidney organoids have potential uses in disease modelling, drug screening and regenerative medicine...

...Rajasree Menon; Edgar A. Otto; Austin Kokoruda; Jian Zhou; Zidong Zhang; Euisik Yoon; Yu-Chih Chen; Olga Troyanskaya; Jason R. Spence; Matthias Kretzler; Cristina Cebrián ABSTRACT The mammalian kidney develops through reciprocal interactions between the ureteric bud and the metanephric mesenchyme...

...Audrey Desgrange; Claire Heliot; Ilya Skovorodkin; Saad U. Akram; Janne Heikkilä; Veli-Pekka Ronkainen; Ilkka Miinalainen; Seppo J. Vainio; Silvia Cereghini Kidney development depends crucially on proper ureteric bud branching giving rise to the entire collecting duct system. The transcription...

...Sijo Mathew; Riya J. Palamuttam; Glenda Mernaugh; Harini Ramalingam; Zhenwei Lu; Ming-Zhi Zhang; Shuta Ishibe; David R. Critchley; Reinhard Fässler; Ambra Pozzi; Charles R. Sanders; Thomas J. Carroll; Roy Zent Kidney collecting system development requires integrin-dependent cell-extracellular...

... coverslip and the spacers adjust the thickness, enabling the tissue to grow for up to 12 days. Thus, the kidney rudiment and the organoids can adjust to the limited z -directional space and yet advance the process of kidney morphogenesis, enabling long-term time-lapse and high-resolution confocal imaging...

.... Summary: Cellular senescence is an intrinsic part of the developmental programme in amphibians and has a conserved role in vertebrate organogenesis. Cellular senescence Axolotl Xenopus TGFβ Cement gland Kidney Cellular senescence is state of permanent cell cycle arrest...

...Eunah Chung; Patrick Deacon; Sierra Marable; Juhyun Shin; Joo-Seop Park During nephrogenesis, multipotent mesenchymal nephron progenitors develop into distinct epithelial segments. Each nephron segment has distinct cell types and physiological function. In the current model of kidney development...

...Yaopan Mao; Philippa Francis-West; Kenneth D. Irvine Formation of the kidney requires reciprocal signaling among the ureteric tubules, cap mesenchyme and surrounding stromal mesenchyme to orchestrate complex morphogenetic events. The protocadherin Fat4 influences signaling from stromal to cap...

... are bound to promoters of key renal developmental regulators and that HDAC activity is required for embryonic kidney gene expression. However, the existence of many HDAC isoforms in embryonic kidneys raises questions concerning the possible specificity or redundancy of their functions. We report here...

.... Activity Cre Neuron Notch1 Kidney The Notch signaling pathway is an evolutionarily conserved, short-range communication mechanism utilized throughout life in all metazoan species ( Artavanis-Tsakonas et al., 1999 ). The binding of a ligand to a Notch receptor triggers the unfolding...

...Wiebke Cizelsky; Aleksandra Tata; Michael Kühl; Susanne J. Kühl Proper development of nephrons is essential for kidney function. β-Catenin-independent Wnt signaling through Fzd8, Inversin, Daam1, RhoA and Myosin is required for nephric tubule morphogenesis. Here, we provide a novel mechanism...