... nervous system. ( A-H ) SEM analysis of wild-type (A,B,E,F) and Hes1;Hes5 double-null (C,D,G,H)embryos. Parts of A and C are enlarged in B and D, respectively. At E10.5, the ZLI (arrows) and the isthmus (arrowhead) are clearly formed in the wild type(A,B). By contrast, in Hes1;Hes5 double-null embryo...

... to this work 21 9 2005 ©2005. 2005 Cell cycle Neuron Differentiation ATBF1 ATM Isthmus Rat AT motif-binding factor 1 (ATBF1) is a 404 kDa transcription factor that contains four homeodomains and 23 zinc-finger motifs( Miura et al., 1995 ). A small alternatively-spliced...

.... Single morpholino knockdown of either Lmx1b.1 or Lmx1b.2 has no discernible effect on MMR development. Maintenance of lmx1b.1 and lmx1b.2 expression at the isthmus requires the function of no isthmus / pax2.1 , as well as Fgf signaling. Transient misexpression of Lmx1b.1 or Lmx1b.2 during early MMR...

...Asuka Suzuki-Hirano; Tatsuya Sato; Harukazu Nakamura Fgf8 functions as an organizer at the mes/metencephalic boundary (isthmus). We showed that a strong Fgf8 signal activates the Ras-ERK signaling pathway to organize cerebellar differentiation. Sprouty2 is expressed in an overlapping manner to Fgf8...

...Tatsuya Sato; Harukazu Nakamura The mes/metencephalic boundary (isthmus) is an organizing center for the optic tectum and cerebellum. Fgf8 is accepted as a crucial organizing signal. Previously, we reported that Fgf8b could induce cerebellum in the mesencephalon, while Fgf8a transformed...

... AmphiDmbx CiDmbx Ciona Chordate MHB Isthmus The complex brain of vertebrates is not only a product of ontogenetic processes, but also of evolutionary history. Unravelling this history can reveal which developmental processes are the most ancient, constituting the fundamental basis...

... domain upon which the cerebellum fuses. Elimination or overgrowth of this isthmic domain in Wnt1 sw/sw and En1 +/Otx2lacZ mutant mice, respectively, impair cerebellar midline fusion. Because the isthmus-derived midline cerebellar domain displays a distinct expression pattern of genes involved in BMP...

... with pax6.1 . Previous experiments have demonstrated that Fgf8 is able to suppress posterior diencephalic fates,assumed to be due to its isthmus-inducing ability( Sato et al., 2001 ). In ace mutant embryos with a non-functional Fgf8 protein or in MO- fgf8 injected embryos, the pax6.1 expression domain...

... fates. * Author for correspondence (e-mail: cliff@drugs.bsd.uchicago.edu ) 12 9 2002 ©2002. 2002 Bain nuclei Oculomotor neurons Red nucleus Homeobox genes Emx2 Sonic hedgehog FGF8 Isthmus Chick Mouse In the adult brain, many neurons are organized into well...

... and also immediately posterior to it in anterior rhombomere 1. Axons of the most anterior cells follow a dorsal projection, which circumnavigates the isthmus, while those of more posterior trochlear neurons project anterodorsally to enter the isthmus. Once within the isthmus, axons form large fascicles...

...Eiji Matsunaga; Tatsuya Katahira; Harukazu Nakamura The isthmus is the organizing center for the tectum and cerebellum. Fgf8 and Wnt1 are secreted molecules expressed around the isthmus. The function of Fgf8 has been well analyzed, and now accepted as the most important organizing signal...

... of pax2.1 . Comparison of transgene expression in wild-type and homozygous pax2.1 mutant no isthmus (noi) embryos reveals that the transgene contains control element(s) for a novel, positive transcriptional feedback loop in MHB development. Transcription of endogenous pax2.1 at the MHB is known...

... of no isthmus (noi) alleles of the zebrafish pax2.1 gene reveals multiple signaling events in development of the midbrain- hindbrain boundary. Development 125 , 3049 -3062. Mahmood, R., Mason, I. J. and Morriss-Kay, G. M. ( 1996 ). Expression of Fgf-3 in relation to hindbrain segmentation, otic pit...

... Competence Fgf8 pou2 Oct3/4 Pou5f1 MHB Isthmus pax2 spiel-ohne-grenzen Hindbrain Zebrafish The organization of the vertebrate brain and its differentiation into functionally and anatomically distinct areas is based on early patterning and regional specification of the neural plate...

... expression domains positions the MHB. How the positional information is translated into activation of Pax2 , Wnt1 and Fgf8 expression during MHB establishment remains unclear. In zebrafish spiel ohne grenzen ( spg ) mutants, the MHB is not established, neither isthmus nor cerebellum form, the midbrain...

... (the isthmus) located at the midbrain/hindbrain boundary confronts segmentation of the hindbrain. The unique developmental fate of rhombomere 1 is reflected by it being the only hindbrain segment in which no Hox genes are expressed. In this study we show that ectopic FGF8 protein, a candidate for the isthmic...

...Carol Irving; Ivor Mason ABSTRACT The midbrain-hindbrain boundary, or isthmus, is the source of signals that are responsible for regional specification of both the midbrain and anterior hindbrain. Fibroblast growth factor 8 (Fgf8) is expressed specifically at the isthmus and there is now good...

... boundary (MHB), a region from which a peculiar structure, the isthmus, is formed. Knock-out experiments in mice demonstrated that these genes are essential for the development of this structure and of its derivatives. In contrast, little is known about the effect of an overexpression of engrailed genes...

... of En1 in neural plate explants in vitro and FGF4 mimics this effect of notochord tissue. Subsequently, a second member of the fibroblast growth factor family, Fgf8 , is expressed in the isthmus in a manner consistent with it providing a polarising signal for the developing midbrain. Using a retroviral...

... structures such as eyes and the anterior neural ridge were lost by 8.5 dpc, but the isthmus and r1 and r2 were formed. In regionalization of the rostral neuroectoderm, the cooperative interaction of Otx2 with Otx1 revealed by the phenotype of Otx2 and Otx1 double heterozygotes was substitutable by Otx1...