...Olesya Sokolova; Valeriya Morgunova; Tatyana V. Sizova; Pavel A. Komarov; Oxana M. Olenkina; Dmitry S. Babaev; Elena A. Mikhaleva; Dmitry A. Kwon; Maksim Erokhin; Alla Kalmykova ABSTRACT Insulators are architectural elements implicated in the organization of higher-order chromatin structures...

... impact BX-C gene regulation and segment specification. Insulator Chromatin organization Zinc-finger transcription factors Protein-protein interactions Architectural proteins Russian Science Foundation 10.13039/501100006769 14-24-00166 National Institutes of Health...

... specification is incompletely understood. In this article, we identify the insulator protein BEAF-32 as a regulator of Hippo pathway activity in Drosophila photoreceptor differentiation. Though morphologically uniform, the fly eye is composed of two subtypes of R8 photoreceptor neurons defined by expression...

...Mamiko Yajima; William G. Fairbrother; Gary M. Wessel Insulators are genomic elements that regulate transcriptional activity by forming chromatin boundaries. Various DNA insulators have been identified or are postulated in many organisms, and the paradigmatic CTCF-dependent insulators are perhaps...

...Martin Herold; Marek Bartkuhn; Rainer Renkawitz The genome of higher eukaryotes exhibits a patchwork of inactive and active genes. The nuclear protein CCCTC-binding factor (CTCF) when bound to insulator sequences can prevent undesirable crosstalk between active and inactive genomic regions...

...Miki Fujioka; Xian Wu; James B. Jaynes Insulator sequences help to organize the genome into discrete functional regions by preventing inappropriate cross-regulation. This is thought to be mediated in part through associations with other insulators located elsewhere in the genome. Enhancers...

...Carole Iampietro; Fabienne Cléard; Henrik Gyurkovics; Robert K. Maeda; François Karch Although the boundary elements of the Drosophila Bithorax complex(BX-C) have properties similar to chromatin insulators, genetic substitution experiments have demonstrated that these elements do more than simply...

... constructs revealed the existence of several types of regulatory elements in each of the cis-regulatory regions. These include initiation elements, maintenance elements, cell type- or tissue-specific enhancers, chromatin insulators and the promoter targeting sequence. In this paper, we extend the analysis...

... HEAT Repeat Insulator Scc3/stromalin Smc1 Spo76 Current evidence indicates that most transcriptional activator proteins recruit the basal transcriptional machinery and increase its binding to a promoter ( Ptashne and Gann,2001 ). When an activator binds DNA within a kilobase or so...

... (domains) of genetic regulation that boundaries delimit can expand or contract by switching insulator function off or on in a temporally regulated fashion. * Author for correspondence (e-mail: [email protected] ) 8 7 2004 © 2004. 2004 Fab-7 Drosophila Bithorax...

...Anton Golovnin; Inna Biryukova; Olga Romanova; Margarita Silicheva; Akeksander Parshikov; Ekaterina Savitskaya; Vincenzo Pirrotta; Pavel Georgiev The best characterized chromatin insulator in Drosophila is the Suppressor of Hairy wing binding region contained within the gypsy retrotransposon...

... complex facilitates the activity of a distantly located enhancer in transgenic embryos and that it restricts the enhancer to a single promoter. These functions are heritable in all successive generations. We also show that the PTS functions only when itself and an insulator are located between...

... sequences located at remote distances. However, in different cellular contexts, Hox genes may have to be insulated from undesirable external regulatory influences to prevent ectopic gene activation, a situation that would likely be detrimental to the developing embryo. We show the presence of an insulator...

...Ilham Hogga; François Karch The Fab-7 chromatin domain boundary insures functional autonomy of the iab-6 and iab-7 cis -regulatory domains in the bithorax complex (BX-C). We have previously shown that chromatin insulators such as gypsy or scs min are potent insulators that cannot substitute...

...Haini N. Cai; Zhengang Zhang; Jessica R. Adams; Ping Shen Chromatin insulators regulate gene expression by preventing inappropriate enhancer-promoter interactions. Our previous study showed that insulators do not merely function as rigid blockers, rather their activities are quantitative...

... that specify PS13 identity are located on the distal side of Fab-8 , in iab-8 . We use transgene assays to demonstrate that Fab-8 has enhancer blocking activity and that it can insulate reporter constructs from the regulatory action of the iab-7 and iab-8 initiators. We also show that the Fab-8 boundary...

... inserted into a variety of P -transformation vectors. Analyses of reporter gene expression in transgenic embryos and adults identify at least three cis-regulatory elements, including two enhancers (IAB7 and IAB8) and a new insulator DNA ( Frontabdominal-8, Fab-8 ). Evidence is also presented for a Polycomb...