...:   Drosophila adult fat body precursor cells form adult adipose tissue during metamorphosis by directional migration, continuous cell proliferation and homotypic cell fusion. Insect development Adult fat body Adipose tissue Directional migration Cell proliferation In vivo imaging Japan...

.... The use of advanced in vivo live imaging approaches may expand our knowledge of NSC physiology and provide new information for cell replacement therapies. In this Review, we discuss the in vivo imaging methods used to study NSC dynamics and recent live-imaging results with respect to specific...

... mechanosensory neurons, the functional counterparts to vertebrate hair cells, and their enhanced regeneration through pharmacological administration. Regeneration Nervous system Auditory neuron Vestibular neuron Lineage tracing In vivo imaging Hearing and balance disorders affect over 5...

... cells In vivo imaging Extracellular matrix Zebrafish Tissue-resident stem cells, such as muscle stem cells, are crucial for proper organ development and tissue homeostasis. To harness the power of stem cells for treating diseases such as muscular dystrophy, it is critical to understand how...

... to prevent the spread of tissue damage in the aftermath of a brain injury by migrating to the lesion site and clearing it of dead cells. Brain injury Secondary cell death Microglia Phagocytosis In vivo imaging Zebrafish Traumatic brain injury (TBI) is a leading cause of death...

... to form an FT–FD complex is lacking. Here, using an innovative in vivo imaging technique, we show that the FT–FD complex and AP1 colocalise in floral anlagen. In addition, the FT–FD complex disappears soon after the floral transition owing to a reduction in FD transcripts in the shoot apex. We further...

.... In this Spotlight article, we highlight how in vivo imaging can probe a range of biological processes that relate to stem cell activity, behaviour and control. References Andrews , N. , Ramel , M.-C. , Kumar , S. , Alexandrov , Y. , Kelly , D. J. , Warren , S. C. , Kerry , L...

..., an in vivo chitin reporter. Chitin reporter Cuticle Drosophila In vivo imaging Chitin is generally considered to be the second most abundant biomolecule. It is a polymer of N-acetylglucosamine linked by β-1,4 glycosidic bonds and is synthesized by the enzyme chitin synthase, which...

... S1pr2/Gα 13 In vivo imaging In Gα 13 -deficient embryos, the ALPM cells flanked the widened endodermal layer, and those in the dorsalmost regions, including the myocardial cells, failed to migrate to the midline by 22 s, leaving a gap ( Fig. 9 L, yellow and red lines; supplementary material...

... in zebrafish embryos reveals that the segmentation clock shifts from one- to two-segment periodicity, suggesting an updated model for somite formation. Two-segment periodicity Segmentation clock Zebrafish In vivo imaging her1 Somite Oscillations In vertebrates, the process...

... Drosophila wing Growth Image analysis In vivo imaging Mathematical analysis Morphogenesis Attempts to immobilize Drosophila larvae for imaging have already been performed. They consist of either squeezing ( Ghannad-Rezaie et al., 2012 ; Nienhaus et al., 2012 ) or anesthetizing ( Füger et...

... processes. Among vertebrates, zebrafish is uniquely suited for in vivo imaging owing to its small size and optical translucency. However, distinguishing and following cells over extended time periods remains difficult. Previous studies have demonstrated that Cre recombinase-mediated recombination can lead...

... applicable to more fields of developmental biology, in particular the multiview long-term imaging of zebrafish embryos and other small organisms. Furthermore, the refinement of sample preparation for in toto and in vivo imaging will promote other emerging optical imaging techniques, such as optical...

... ( david.lyons@ed.ac.uk ) * These authors contributed equally to this work Competing interests statement The authors declare no competing financial interests. 29 7 2011 © 2011. CNS Myelination In vivo imaging Oligodendrocyte Zebrafish The vast majority...

... apoptosis are marked by yellow dots. Magenta dots show cells located in A8pp, which moved with A9, and green dots represent cells in A8pa, which rotated later. Genotype was en-GAL4 UAS-nls-SCAT3/+; His2Av-mRFP/+. Three flies were examined and a typical example is shown. Fig. 1. In vivo imaging...

... © 2010. Fibroblast growth factors Cell migration Intercalation In vivo imaging Controlled cell migration is an essential aspect of development in which cells relocate to respond to chemical signals and form structures ( Lecaudey and Gilmour, 2006 ; Rorth, 2007 ; Montell...

... ) * These authors contributed equally to this work 18 4 2006 © 2006. 2006 Xenopus laevis Synapse Tectal neuron PSD95-GFP Branching In vivo imaging APV In the developing central nervous system, functional neuronal circuitry is established as axon terminals arborize, recognize...

..., by a mechanism that influences both synapses and axon branches. * Author for correspondence (e-mail: scohenco@uci.edu ) 29 7 2005 ©2005. 2005 Xenopus laevis Retinal ganglion cell Axon branching In vivo imaging During development, synapses form, mature and stabilize...

... Cis-regulatory sequence Synteny In vivo imaging Zebrafish The recent analysis of the human and several other vertebrate genomes has brought with it significant advances in the identification of evolutionarily conserved, and therefore functional, sequences. For example, comparison...

... not linked to a mechanism that generates asymmetric divisions. * Author for correspondence (e-mail: jonathan.clarke@ucl.ac.uk ) 25 4 2003 © 2003. 2003 In vivo imaging Vertebrate neurogenesis Neural progenitor cell fate Asymmetric division Zebrafish During development...