... and mitochondrial Ca 2+ activity in live zebrafish during development reveals that gut microbiota play crucial roles in promoting EEC maturation and mitochondrial function. Enteroendocrine cells Gut microbiota Mitochondria In vivo imaging Zebrafish National Institutes of Health http...

... Transcriptional noise Optogenetics Collective behaviour In vivo imaging Excitable signalling Wellcome Trust http://dx.doi.org/10.13039/100010269 202867/Z/16/Z 226655/Z/22/Z Medical Research Council MC_U12266B The changes in gene expression occurring during developmental...

... tissue Directional migration Cell proliferation In vivo imaging Japan Agency for Medical Research and Development http://dx.doi.org/10.13039/100009619 Core Research for Evolutional Science and Technology http://dx.doi.org/10.13039/501100003382 JP18gm1110001 Japan...

.... The use of advanced in vivo live imaging approaches may expand our knowledge of NSC physiology and provide new information for cell replacement therapies. In this Review, we discuss the in vivo imaging methods used to study NSC dynamics and recent live-imaging results with respect to specific...

... mechanosensory neurons, the functional counterparts to vertebrate hair cells, and their enhanced regeneration through pharmacological administration. Regeneration Nervous system Auditory neuron Vestibular neuron Lineage tracing In vivo imaging Hearing and balance disorders affect over 5...

.... Dermomyotome Somite Muscle progenitor cells In vivo imaging Extracellular matrix Zebrafish Canadian Institute of Health Research 10.13039/501100000024 MOP-136926 PJT-156035 Canada Foundation for Innovation 10.13039/501100001805 Project 32920 Alberta Children's...

... to prevent the spread of tissue damage in the aftermath of a brain injury by migrating to the lesion site and clearing it of dead cells. Brain injury Secondary cell death Microglia Phagocytosis In vivo imaging Zebrafish Biogen 10.13039/100005614 University of Edinburgh...

... to form an FT–FD complex is lacking. Here, using an innovative in vivo imaging technique, we show that the FT–FD complex and AP1 colocalise in floral anlagen. In addition, the FT–FD complex disappears soon after the floral transition owing to a reduction in FD transcripts in the shoot apex. We further...

.... In this Spotlight article, we highlight how in vivo imaging can probe a range of biological processes that relate to stem cell activity, behaviour and control. * Author for correspondence ( [email protected] ) Competing interests The authors declare no competing or financial interests. © 2018...

..., an in vivo chitin reporter. Chitin reporter Cuticle Drosophila In vivo imaging Chitin is generally considered to be the second most abundant biomolecule. It is a polymer of N-acetylglucosamine linked by β-1,4 glycosidic bonds and is synthesized by the enzyme chitin synthase, which...

... S1pr2/Gα 13 In vivo imaging Interplay between adjacent tissues is crucial for organ formation. During vertebrate development, the heart tube is formed by the fusion of two populations of myocardial precursors, which migrate from bilateral regions of embryos. This migration requires proper...

... in zebrafish embryos reveals that the segmentation clock shifts from one- to two-segment periodicity, suggesting an updated model for somite formation. Two-segment periodicity Segmentation clock Zebrafish In vivo imaging her1 Somite Oscillations In vertebrates, the process...

... Drosophila wing Growth Image analysis In vivo imaging Mathematical analysis Morphogenesis Attempts to immobilize Drosophila larvae for imaging have already been performed. They consist of either squeezing ( Ghannad-Rezaie et al., 2012 ; Nienhaus et al., 2012 ) or anesthetizing ( Füger et...

... processes. Among vertebrates, zebrafish is uniquely suited for in vivo imaging owing to its small size and optical translucency. However, distinguishing and following cells over extended time periods remains difficult. Previous studies have demonstrated that Cre recombinase-mediated recombination can lead...

... applicable to more fields of developmental biology, in particular the multiview long-term imaging of zebrafish embryos and other small organisms. Furthermore, the refinement of sample preparation for in toto and in vivo imaging will promote other emerging optical imaging techniques, such as optical...

... ( [email protected] ) * These authors contributed equally to this work Competing interests statement The authors declare no competing financial interests. 29 7 2011 © 2011. CNS Myelination In vivo imaging Oligodendrocyte Zebrafish The vast majority...

... apoptosis are marked by yellow dots. Magenta dots show cells located in A8pp, which moved with A9, and green dots represent cells in A8pa, which rotated later. Genotype was en-GAL4 UAS-nls-SCAT3/+; His2Av-mRFP/+. Three flies were examined and a typical example is shown. Fig. 1. In vivo imaging...

... © 2010. Fibroblast growth factors Cell migration Intercalation In vivo imaging Controlled cell migration is an essential aspect of development in which cells relocate to respond to chemical signals and form structures ( Lecaudey and Gilmour, 2006 ; Rorth, 2007 ; Montell...

... ) * These authors contributed equally to this work 18 4 2006 © 2006. 2006 Xenopus laevis Synapse Tectal neuron PSD95-GFP Branching In vivo imaging APV In the developing central nervous system, functional neuronal circuitry is established as axon terminals arborize, recognize...

..., by a mechanism that influences both synapses and axon branches. * Author for correspondence (e-mail: [email protected] ) 29 7 2005 ©2005. 2005 Xenopus laevis Retinal ganglion cell Axon branching In vivo imaging During development, synapses form, mature and stabilize...