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Keywords: In utero electroporation
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Journal Articles
Journal: Development
Development (2017) 144 (24): 4604–4615.
Published: 15 December 2017
..., neuron-specific knockdown of LRP4 by in utero electroporation of LRP4 miRNA in vivo also resulted in neurons with fewer primary dendrites and a lower density of spines in the developing cortex and hippocampus. Collectively, our results demonstrate an essential and novel role of neuronal LRP4 in dendritic...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (9): 1698–1711.
Published: 1 May 2017
..., Dlx1 / Dlx2 / Brn3b triple-knockout mice exhibit near complete RGC loss. Homeobox Chromatin immunoprecipitation In utero electroporation Atoh7 Math5 Mouse Six classes of neurons and Müller glial cells are generated from a common retinal progenitor cell (RPC) population...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2016) 143 (17): 3216–3222.
Published: 1 September 2016
...Yuji Tsunekawa; Raymond Kunikane Terhune; Ikumi Fujita; Atsunori Shitamukai; Taeko Suetsugu; Fumio Matsuzaki Genome-editing technology has revolutionized the field of biology. Here, we report a novel de novo gene-targeting method mediated by in utero electroporation into the developing mammalian...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2016) 143 (6): 1029–1040.
Published: 15 March 2016
... phosphorylation on neuronal migration we used in utero electroporation to transfect E14.5 wild-type cortical neurons with pCAG-EGFP plasmids encoding either a nonphosphorylatable form of cofilin 1 ( cofilin S3A ), a pseudophosphorylated form ( cofilin S3E ) or wild-type cofilin 1 ( cofilin WT ). Wild-type...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2015) 142 (21): 3746–3757.
Published: 1 November 2015
... h and luciferase activity was measured using the Dual-Luciferase Reporter Assay System (Promega) on a POLARstar OPTIMA plate reader (BMG Labtech). Cloning of luciferase DNA constructs are described in Table S1 and the supplementary materials and methods . In utero electroporation...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2015) 142 (20): 3601–3611.
Published: 15 October 2015
... their function in the developing mouse brain. CRISPR/Cas9 Neural progenitors Lineage piggyBac In utero electroporation Glioblastoma multiforme A general approach to studying developmental mechanisms is to mutate genes of interest, and then evaluate the resulting phenotypes. Recent...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2007) 134 (24): 4369–4380.
Published: 15 December 2007
...:10,000; Boehringer Mannheim). In utero electroporation was performed as described with the square electroporator CUY21 EDIT (TR Tech, Japan), delivering five 50 ms pulses of 40-50 V with 950 ms intervals( Barnabé-Heider et al.,2005 ; Paquin et al.,2005 ; Gauthier et al.,2007 ). E13/E14 CD1 mice...
Journal Articles
Journal: Development
Development (2004) 131 (22): 5639–5647.
Published: 15 November 2004
...Tomomi Shimogori; Victoria Banuchi; Hanyann Y. Ng; Jonathan B. Strauss; Elizabeth A. Grove Recent findings implicate embryonic signaling centers in patterning the mammalian cerebral cortex. We used mouse in utero electroporation and mutant analysis to test whether cortical signaling sources...