... approach (Brain Easi-CRISPR), combining Easi-CRISPR with in utero electroporation to tag endogenous proteins within embryonic mouse brains. Breasi-CRISPR enables knock-in of both short and long epitope tag sequences with high efficiency. We visualized epitope-tagged proteins with varied expression levels...

..., neuron-specific knockdown of LRP4 by in utero electroporation of LRP4 miRNA in vivo also resulted in neurons with fewer primary dendrites and a lower density of spines in the developing cortex and hippocampus. Collectively, our results demonstrate an essential and novel role of neuronal LRP4 in dendritic...

... ( Fig. 9 K). These in utero gain-of-function experiments support that exogenous Dlx2 gene function is sufficient for Brn3b expression in the embryonic retina in vivo . Fig. 9. In utero electroporation of Dlx2 results in ectopic Brn3b expression in vivo . E14.5 retinas...

...Yuji Tsunekawa; Raymond Kunikane Terhune; Ikumi Fujita; Atsunori Shitamukai; Taeko Suetsugu; Fumio Matsuzaki Genome-editing technology has revolutionized the field of biology. Here, we report a novel de novo gene-targeting method mediated by in utero electroporation into the developing mammalian...

... phosphorylation on neuronal migration we used in utero electroporation to transfect E14.5 wild-type cortical neurons with pCAG-EGFP plasmids encoding either a nonphosphorylatable form of cofilin 1 ( cofilin S3A ), a pseudophosphorylated form ( cofilin S3E ) or wild-type cofilin 1 ( cofilin WT ). Wild-type...

... expression of the guidance receptor NRP1. Anterograde tracing Axon guidance Cerebral cortex Corpus callosum In utero electroporation Tractography The anterior cingulate cortex in humans and mice is particularly important for the performance of executive functions and emotional processing...

... their function in the developing mouse brain. CRISPR/Cas9 Neural progenitors Lineage piggyBac In utero electroporation Glioblastoma multiforme A general approach to studying developmental mechanisms is to mutate genes of interest, and then evaluate the resulting phenotypes. Recent...

... than previously thought. † Author for correspondence (e-mail: [email protected] ) * These authors contributed equally 14 9 2007 © 2007. 2007 TrkB TrkC BDNF Neurogenesis Gliogenesis Self-renewal Cortical precursors Neural stem cells In utero electroporation Akt...

...Tomomi Shimogori; Victoria Banuchi; Hanyann Y. Ng; Jonathan B. Strauss; Elizabeth A. Grove Recent findings implicate embryonic signaling centers in patterning the mammalian cerebral cortex. We used mouse in utero electroporation and mutant analysis to test whether cortical signaling sources...