...Audrey Savolainen; Emmi Kapiainen; Veli-Pekka Ronkainen; Valerio Izzi; Martin M. Matzuk; Diana Monsivais; Renata Prunskaite-Hyyryläinen ABSTRACT Effective interplay between the uterus and the embryo is essential for pregnancy establishment; however, convenient methods to screen embryo implantation...

...Joanne Muter; Vincent J. Lynch; Rajiv C. McCoy; Jan J. Brosens ABSTRACT Embryo implantation in humans is interstitial, meaning the entire conceptus embeds in the endometrium before the placental trophoblast invades beyond the uterine mucosa into the underlying inner myometrium. Once implanted...

... sites that permit implantation. When translating systems biology of the undifferentiated bovine trophectoderm, we uncovered that inhibition of RhoA/Rock promoted self-renewing proliferation and substantially increased blastocyst size. Analysis of transcripts suppressed by Rock inhibition revealed...

...Grace V. Hancock; Sissy E. Wamaitha; Lior Peretz; Amander T. Clark ABSTRACT The peri-implantation window of mammalian development is the crucial window for primordial germ cell (PGC) specification. Whereas pre-implantation dynamics are relatively conserved between species, the implantation window...

... along the longitudinal oviductal-cervical axis of murine uteri. Our analysis reveals three distinct pre-implantation phases: embryo entry, unidirectional movement of embryo clusters and bidirectional scattering and spacing of embryos. We show that unidirectional clustered movement is facilitated...

...Ripla Arora; Adam Fries; Karina Oelerich; Kyle Marchuk; Khalida Sabeur; Linda C. Giudice; Diana J. Laird Although much is known about the embryo during implantation, the architecture of the uterine environment in which the early embryo develops is not well understood. We employed confocal imaging...

..., Gata3 is coexpressed with Cdx2 from the earliest developmental stages. Since TS cells have also been derived from post-implantation embryos, around the time of gastrulation ( Tanaka et al., 1998 ; Uy et al., 2002 ), we next examined Gata3 expression around gastrula stages by in situ hybridization...

...Mary J. Laws; Robert N. Taylor; Neil Sidell; Francesco J. DeMayo; John P. Lydon; David E. Gutstein; Milan K. Bagchi; Indrani C. Bagchi In the uterus, the formation of new maternal blood vessels in the stromal compartment at the time of embryonic implantation is critical for the establishment...

...Huirong Xie; Susanne Tranguch; Xiangxu Jia; Hao Zhang; Sanjoy K. Das; Sudhansu K. Dey; Calvin J. Kuo; Haibin Wang The activation of the blastocyst, a process by which it gains competency to attach with the receptive uterus, is a prerequisite for successful implantation. However, the molecular basis...

... of Furin to the extra-embryonic region. Activation of Nodal is also necessary to maintain determinants of pluripotency such as Oct4, Nanog and Foxd3 during implantation, and to stimulate elongation of the egg cylinder, before inducing DVE and germ layer formation. We conclude that Nodal is already...

...Helene Solberg; Julie Rinkenberger; Keld Danø; Zena Werb; Leif R. Lund Both plasminogen activators and matrix metalloproteinases (MMPs) have been implicated in a variety of developmental processes in the mouse during embryo implantation and placentation. We show here that pharmacological treatment...

...Xiaohong Wang; Haibin Wang; Hiromichi Matsumoto; Shyamal K. Roy; Sanjoy K. Das; Bibhash C. Paria Heparin binding EGF-like growth factor (HB-EGF), encoded by the Hegfl gene, is considered as an important mediator of embryo-uterine interactions during implantation in mice. However, it is unknown...

... (PGs). Female mice with the null mutation for Pla2g4a (cPLA 2 α) produce small litters and often exhibit pregnancy failures, although the cause(s) of these defects remains elusive. We show that the initiation of implantation is temporarily deferred in Pla2g4a –/– mice, shifting the normal ‘window...

...Xuemei Zhao; Wen-ge Ma; Sanjoy K. Das; Sudhansu K. Dey; Bibhash C. Paria ABSTRACT The process of implantation is a ‘two-way’ interaction between the blastocyst and uterus. It has long been suspected that histamine is an important mediator in embryo-uterine interactions during implantation, but its...

...Jun Wang; Linda Mayernik; Jefferey F. Schultz; D. Randall Armant ABSTRACT Heparin-binding EGF-like growth factor (HB-EGF) is expressed in the mouse endometrial epithelium during implantation exclusively at sites apposed to embryos and accelerates the development of cultured blastocysts, suggesting...

...Jun Wang; Ujjwal K. Rout; Indrani C. Bagchi; D. Randall Armant ABSTRACT Calcitonin secretion in the pregnant uterus is tightly regulated by the ovarian hormones, estrogen and progesterone, which limit its expression to a brief period preceding blastocyst implantation. The binding of calcitonin...

...Suzanne Afonso; Linda Romagnano; Bruce Babiarz ABSTRACT The implantation of the mouse embryo requires the controlled invasion of the uterine stroma by the embryonic trophoblast. This event is dependent, in part, on the secretion of matrix metalloproteinases and serine proteinases...

...Gail V. Benson; Hyunjung Lim; B. C. Paria; Ichiro Satokata; Sudhansu K. Dey; Richard L. Maas ABSTRACT The establishment of a receptive uterine environment is critical for embryonic survival and implantation. One gene that is expressed in the uterus during the peri-implantation period in mice...

... of this enzyme activity inhibits lysis of extracellular matrix (Behrendtsen, O., Alexander, C. M. and Werb, Z. (1992) Development 114, 447-456). Because gelatinase B expression is linked to invasive potential, we studied the expression of gelatinase B mRNA and protein in vivo, in implanting trophoblast giant...

... metalloproteinase gelatinase B (MMP-9), urokinase-type plasminogen activator (uPA) and their inhibitors was investigated during early mouse embryo development. Transcripts for tissue inhibitors of metalloproteinases (TIMP-1,-2,-3) and uPA receptor were detected throughout pre- and peri-implantation develop-ment...