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Keywords: Image analysis pipeline
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Journal Articles
Automated detection and quantification of single RNAs at cellular resolution in zebrafish embryos
FreeL. Carine Stapel, Benoit Lombardot, Coleman Broaddus, Dagmar Kainmueller, Florian Jug, Eugene W. Myers, Nadine L. Vastenhouw
Journal:
Development
Development (2016) 143 (3): 540–546.
Published: 1 February 2016
... of efficient image analysis pipelines have hampered quantification at the (sub)cellular level in complex samples such as tissues and embryos. Here, we present a protocol for smFISH on zebrafish embryo sections in combination with an image analysis pipeline for automated transcript detection and cell...
Includes: Supplementary data
