... network analyses of temporally resolved scRNA-seq data revealed fibroblast-derived insulin-like growth factor 2 (IGF2) as a candidate driving neonatal thymic expansion. We demonstrated IGF2 activity promotes a cortical TEC-specific proliferation and is tightly regulated at the thymic growth transition...

... understood. Recently, an IGF2 mutation was identified in humans with early postnatal growth restriction. Here, we show that IGF2 is essential for longitudinal and appositional murine postnatal bone development, which involves proper timing of chondrocyte maturation and perichondrial cell differentiation...

...Sylvie Nathalie Hardouin; Ruolin Guo; Paul-Henri Romeo; Andras Nagy; Jane E. Aubin During embryonic development, Igf2 gene transcription is highly regulated through the use of several promoters whose specific roles are not defined. Here, we show that loss-of-function of one of these promoters, Igf2...

... with EPO, but not RA, upregulates Igf2 . Furthermore, both Raldh2 –/– and Rxra –/– hearts exhibit downregulation of Igf2 mRNA in the epicardium. EPO treatment of cultured Raldh2 –/– hearts restores epicardial Igf2 expression and rescues ventricular cardiomyocyte proliferation. We propose a new model...

... phenotype, due to a cis effect of the H19 locus on the adjacent Igf2 gene. To explore the function of the RNA itself, we produced transgenic mice overexpressing H19 . We observed postnatal growth reduction in two independent transgenic lines and detected a decrease of Igf2 expression in embryos...

... cluster of imprinted genes. We show that the mutation is an inversion, whose breakpoint distal to H19 disrupts and thus identifies an enhancer for Igf2 expression in skeletal muscle and tongue, and separates the gene from other mesodermal and extra-embryonic enhancers. Paternal transmission of Mnt leads...

...Robert A. Drewell; Katharine L. Arney; Takahiro Arima; Sheila C. Barton; James D. Brenton; M. Azim Surani The reciprocally imprinted H19 and Igf2 genes form a co-ordinately regulated 130 kb unit in the mouse controlled by widely dispersed enhancers, epigenetically modified silencers...

...Justin F-X. Ainscough; Rosalind M. John,; Sheila C. Barton; M. Azim Surani ABSTRACT Igf2 and H19 are closely linked and reciprocally expressed genes on distal chromosome 7 in the mouse. We have previously shown that a 130 kb YAC transgene contains multiple tissue-specific enhancers for expression...

... , J. F. , Koide , T. , Tada , M. , Barton , S. and Surani , M. A. ( 1997 ). Imprinting of Igf2 and H19 from a 130 kb YAC transgene . Development 124 , 3621 – 3632 . 10.1242/dev.124.18.3621 Ainscough , J. F.-X. , Dandolo , L. and Surani , M. A. H...

...) cells affects their totipotency and may give rise to fetal abnormalities. To investigate whether this is associated with epigenetic alterations in imprinted genes, we analysed two maternally expressed genes ( Igf2r, H19 ) and two paternally expressed genes ( Igf2, U2af1-rs1 ) in ES cells...

... examined Peg1/Mest, Igf2, Peg3, Snrpn, H19, Igf2r and excess p57 KIP2 . We show that paternally expressed genes, Peg1/Mest, Peg3 and Snrpn , are expressed in the parthenotes, presumably due to a lack of maternal epigenetic modifications during oocyte growth. In contrast, the expression of Igf2 , which...

... that the allelic usage of these two genes in normal human placental development may not be directly analogous to the situation previously documented in the mouse embryo. 07 12 1995 © 1996 by Company of Biologists 1996 genomic imprinting H19 IGF2 trophoblast lineage ASISH human placenta...

...Tomas J. Ekström; Hengmi Cui; Xuri Li; Rolf Ohlsson ABSTRACT IGF2 has been shown to be expressed preferentially from the paternally derived allele, although the maternal allele can be found active during both prenatal and postnatal development as well as in neoplastic tumours in humans. We...

...Rolf Ohlsson; Fredrik Hedborg; Lars Holmgren; Colum Walsh; Tomas J. Ekström ABSTRACT The spatial patterns of IGF2 and H19 gene expression are strikingly similar during parts of human embryonic/fetal and early postnatal development. Notable exceptions were found with the ciliary anlage...