...Kristine M. Glauber; Catherine E. Dana; Steve S. Park; David A. Colby; Yukihiko Noro; Toshitaka Fujisawa; A. Richard Chamberlin; Robert E. Steele Developmental processes such as morphogenesis, patterning and differentiation are continuously active in the adult Hydra polyp. We carried out a small...

...Holger Bielen; Sabine Oberleitner; Sylvain Marcellini; Lydia Gee; Patrick Lemaire; Hans R. Bode; Ralph Rupp; Ulrich Technau Homologues of the T-box gene Brachyury play important roles in mesoderm differentiation and other aspects of early development in all bilaterians. In the diploblast Hydra...

... during development. Here, we report the isolation, from a regeneration-specific signal peptide screen, of a novel dickkopf gene from the fresh water cnidarian Hydra . Comparative sequence analysis demonstrates that the Wnt antagonistic subfamily Dkk1/Dkk2/Dkk4 and the non-modulating subfamily Dkk3...

...Mariya Broun; Lydia Gee; Beate Reinhardt; Hans R. Bode Stabilization of β-catenin by inhibiting the activity of glycogen synthase kinase-3β has been shown to initiate axis formation or axial patterning processes in many bilaterians. In hydra, the head organizer is located in the hypostome...

...Toshio Takahashi; Masayuki Hatta; Seungshic Yum; Lydia Gee; Masahiro Ohtani; Toshitaka Fujisawa; Hans R. Bode Hym-301 is a peptide that was discovered as part of a project aimed at isolating novel peptides from hydra. We have isolated and characterized the gene Hym-301 , which encodes this peptide...

... Hydra , we identified a receptor tyrosine kinase ( kringelchen ) with high similarity to FGFR tyrosine kinases. The gene is dynamically upregulated during budding, the asexual propagation of Hydra . Activation occurs in body regions, in which the intrinsic positional value changes. During tissue...

...Dirk Lindgens; Thomas W. Holstein; Ulrich Technau Cnidaria are the first class of organisms in animal evolution with a nervous system. The cnidarian Hydra has two types of neuronal cell,nerve cells and nematocytes. Both differentiate from the same pool of pluripotent stem cells. Yet, the molecular...

...Hiroshi Shimizu; Xiaoming Zhang; Jinsong Zhang; Alexey Leontovich; Kaiyin Fei; Li Yan; Michael P. Sarras, Jr. As a member of the phylum Cnidaria, the body wall of hydra is organized as an epithelium bilayer (ectoderm and endoderm) with an intervening extracellular matrix (ECM). Previous studies...

...Mariya Broun; Hans R. Bode A central process in the maintenance of axial patterning in the adult hydra is the head activation gradient, i.e. the potential to form a secondary axis, which is maximal in the head and is graded down the body column. Earlier evidence suggested that this gradient...

...Jinsong Zhang; Alexey Leontovich; Michael P. Sarras, Jr ABSTRACT A novel putative endothelin-converting enzyme (ECE) has been cloned from hydra, a freshwater invertebrate that belongs to the second oldest phylum of the animal kingdom. As an integral component of the endothelin system, vertebrate...

...Naoe Harafuji; Toshio Takahashi; Masayuki Hatta; Hideo Tezuka; Fumihiro Morishita; Osamu Matsushima; Toshitaka Fujisawa ABSTRACT During the course of a systematic screening of peptide signaling molecules in Hydra magnipapillata , a novel peptide, Hym-323, which enhances foot regeneration...

...Kerry M. Smith; Lydia Gee; Hans R. Bode ABSTRACT Developmental gradients are known to play important roles in axial patterning in hydra. Current efforts are directed toward elucidating the molecular basis of these gradients. We report the isolation and characterization of HyAlx , an aristaless...

...Rainer Deutzmann; Susan Fowler; Xiaoming Zhang; Keneath Boone; Sharon Dexter; Raymond P. Boot-Handford; R. Rachel; Michael P. Sarras, Jr ABSTRACT The body wall of hydra (a member of the phylum Cnidaria) is structurally reduced to an epithelial bilayer with an intervening extracellular matrix (ECM...

...Toshio Takahashi; Osamu Koizumi; Yuki Ariura; Anna Romanovitch; Thomas C. G. Bosch; Yoshitaka Kobayakawa; Shiro Mohri; Hans R. Bode; Seungshic Yum; Masayuki Hatta; Toshitaka Fujisawa ABSTRACT During the course of a systematic screening of peptide signaling molecules in Hydra a novel peptide, Hym...

...Alexey A. Leontovich; Jinsong Zhang; Ken-ichi Shimokawa; Hideaki Nagase; Michael P. Sarras, Jr ABSTRACT As a member of Cnidaria, the body wall of hydra is structurally reduced to an epithelial bilayer with an intervening extracellular matrix (ECM). Biochemical and cloning studies have shown...

... and adult tissue differentiation. We have characterized a new astacin proteinase, hydra metalloproteinase 2 (HMP2) from the Cnidarian, Hydra vulgaris . HMP2 is translated from a single mRNA of 1.7 kb that contains a 1488 bp open reading frame encoding a putative protein product of 496 amino acids...

...Mariya Broun; Sergei Sokol; Hans R. Bode We have isolated Cngsc , a hydra homologue of goosecoid gene. The homeodomain of Cngsc is identical to the vertebrate (65-72%) and Drosophila (70%) orthologues. When injected into the ventral side of an early Xenopus embryo, Cngsc induces a partial secondary...

...Wolfgang Hampe; Jens Urny; Inga Franke; Sabine A. H. Hoffmeister-Ullerich; Doris Herrmann; Claus M. Petersen; Jan Lohmann; H. Chica Schaller ABSTRACT The neuropeptide head activator plays an important role for proliferation and determination of stem cells in hydra. By affinity chromatography a 200...

...Ulrich Technau; Hans R. Bode A homologue of the T-box gene, Brachyury , has been isolated from hydra. The gene, termed HyBra1 , is expressed in the endoderm and is associated with the formation of the hypostome, the apical part of the head in four different developmental situations. In adults...

...Ann Grens; Hiroshi Shimizu; Sabine A. H. Hoffmeister; Hans R. Bode; Toshitaka Fujisawa Signaling molecules affecting patterning processes are usually proteins and rarely peptides. Two novel peptides, pedibin and Hym-346, that are closely related to one another have been isolated from Hydra vulgaris...