... with which to study early human development, partially mimicking peri-implantation stage embryos and offering insights into lineage differentiation, self-organization and morphogenesis. Human pluripotent stem cells Stem cell-based embryo models Implantation Gastrulation National Institutes...

... al. ( 2021 ). Restoration of visual function in advanced disease after transplantation of purified human pluripotent stem cell-derived cone photoreceptors . Cell Rep. 35 , 109022 . 10.1016/j.celrep.2021.109022 Ruzycki , P. A. , Zhang , X. and Chen , S. ( 2018 ). CRX...

... neurons, from human pluripotent stem cells (hPSCs) in an efficient and good manufacturing practice-compliant fashion. This has led to the initiation of several clinical trials using hPSC-derived cells to replace lost or dysfunctional cells, demonstrating evidence of both safety and efficacy. Here, we...

... to generate pure cultures of macrophages that resemble tissue-resident or monocyte-derived subsets. We demonstrate that human pluripotent stem cell-derived extra-embryonic-like and intra-embryonic-like hematopoietic progenitors differentiate into morphologically, transcriptionally and functionally distinct...

... for generating cardiac cell types in vitro from human pluripotent stem cells (hPSCs): embryoid body formation [floating three-dimensional (3D) culture of differentiating aggregates] and directed differentiation of attached cells in 2D culture ( Burridge et al., 2014 ; Gadue et al., 2006 ; Kattman et al...

... into a stratified squamous cell layer, accompanied by the replacement of unspecified mesenchyme with layers of muscle cells. Studies in animal models have provided significant insights into the roles of various signaling pathways in esophageal development. More recent studies using human pluripotent stem cells...

... to neurodegeneration. Thus, it is a crucial parameter in the design of strategies aiming to produce MNs from human pluripotent stem cells (hPSCs) for regenerative medicine/disease modelling applications. However, the in vitro generation of posterior MNs corresponding to the thoracic/lumbosacral spinal cord has been...

.... and Zhang , S. C. ( 2015 ). Generation and expansion of highly pure motor neuron progenitors from human pluripotent stem cells . Nat. Commun.   6 , 6626 . 10.1038/ncomms7626 Eguchi , T. , Tezuka , T. , Miyoshi , S. and Yamanashi , Y. ( 2016 ). Postnatal knockdown of dok-7...

... during human blood cell development are not well understood. We used human pluripotent stem cell reporter lines to track the development of endothelial and haematopoietic populations in an in vitro model of human yolk-sac development. We identified SOX17 − CD34 + CD43 − endothelial cells at day 2...

... the transcription factors TCF21 and WT1. Here, we show that this cell heterogeneity is conserved in human epicardium, regulated by BNC1 and associated with cell fate and function. Single cell RNA sequencing of epicardium derived from human pluripotent stem cells (hPSC-epi) revealed that distinct epicardial...

... proliferation and stratification whereas persistent Yap activation increases cell proliferation and causes aberrant stratification of the developing esophagus. We further demonstrated that the role of YAP signaling is conserved in the developing human esophagus by utilizing 3D human pluripotent stem cell (hPSC...

.... Zack; Melissa C. Skala; David M. Gamm ABSTRACT Numerous protocols have been described for producing neural retina from human pluripotent stem cells (hPSCs), many of which are based on the culture of 3D organoids. Although nearly all such methods yield at least partial segments of retinal structure...

... of these methods in human pluripotent stem cells (hPSCs), and show that generation of sOPTiKD/KO hPSCs is simple, rapid and allows tightly controlled individual or multiplexed gene knockdown or knockout in hPSCs and in a wide variety of differentiated cells. Finally, we illustrate the general applicability...

.... Histone demethylase JMJD3 KDM6B Human pluripotent stem cells Hepatocytes Skeletal muscle Pluripotent stem cells (PSCs), such as human embryonic stem cells (hESCs) ( Thomson et al., 1998 ) and human induced pluripotent stem cells (hiPSCs) ( Takahashi et al., 2007 ; Yu et al., 2007 ), have...

...Derek T. Peters; Christopher A. Henderson; Curtis R. Warren; Max Friesen; Fang Xia; Caroline E. Becker; Kiran Musunuru; Chad A. Cowan ABSTRACT Hepatocyte-like cells (HLCs) are derived from human pluripotent stem cells (hPSCs) in vitro , but differentiation protocols commonly give rise...

...Cathelijne W. van den Berg; Satoshi Okawa; Susana M. Chuva de Sousa Lopes; Liesbeth van Iperen; Robert Passier; Stefan R. Braam; Leon G. Tertoolen; Antonio del Sol; Richard P. Davis; Christine L. Mummery Differentiated derivatives of human pluripotent stem cells (hPSCs) are often considered...

...) and CFs from human pluripotent stem cells (HPSCs) through an intermediate lateral plate mesoderm (LM) stage. HPSCs were initially differentiated to LM in the presence of FGF2 and high levels of BMP4. The LM was robustly differentiated to an epicardial lineage by activation of WNT, BMP and retinoic acid...

...Zengrong Zhu; Danwei Huangfu Developmental biology has long benefited from studies of classic model organisms. Recently, human pluripotent stem cells (hPSCs), including human embryonic stem cells and human induced pluripotent stem cells, have emerged as a new model system that offers unique...

... for correspondence ( [email protected] ) Competing interests statement The authors declare no competing financial interests. 23 1 2012 © 2012. Human pluripotent stem cells Neural commitment MicroRNA The formation of early embryonic tissues is the result of a tightly...

... and reproducible differentiation to occur. The differentiation towards a hepatocyte-like fate appeared to recapitulate many of the developmental stages normally associated with the formation of hepatocytes in vivo. In the current study, we addressed the feasibility of using human pluripotent stem cells to probe...