...Zhe Wang; Akira Numada; Fumi Wagai; Yusuke Oda; Masatoshi Ohgushi; Koichiro Maki; Taiji Adachi; Mototsugu Eiraku ABSTRACT Human pluripotent stem cells (hPSCs) dynamically respond to their chemical and physical microenvironment, dictating their behavior. However, conventional in vitro studies...

... be isolated and expanded, are transcriptionally similar to primary bud tip progenitors, and can differentiate into airway or alveolar organoids. Lung organoid Bud tip progenitor Human pluripotent stem cell Single cell RNA sequencing Chan Zuckerberg Initiative National Heart, Lung...

... a hydrogel that supported the growth of human pluripotent stem cell-derived cortical neurons. Second, we tested and compared two software programs for tracing multi-neuron images in three dimensions and optimized a workflow for neuronal analysis using software that was considered highly suitable...

... the efficient in vitro generation of spinal motor neurons, the induction of the spinal cord as a whole tissue has not been achieved. In the present study, we demonstrate three-dimensional (3D) induction of dorsal spinal cord-like tissues from human pluripotent stem cells. Our 3D spinal cord induction (3-DiSC...

... neurogenesis with a focus on novel technologies, including chemogenetics and optogenetics, live cell two-photon imaging, cell fate reprogramming and human pluripotent stem cell models. This Meeting Review describes the exciting work that was presented and some of the emerging themes from the meeting...

...Audrey Holtzinger; Philip R. Streeter; Farida Sarangi; Scott Hillborn; Maryam Niapour; Shinichiro Ogawa; Gordon Keller The efficient generation of hepatocytes from human pluripotent stem cells (hPSCs) requires the induction of a proper endoderm population, broadly characterized by the expression...

...R. Scott Thies; Charles E. Murry Human pluripotent stem cells (hPSCs) offer many potential applications for drug screening and ‘disease in a dish’ assay capabilities. However, a more ambitious goal is to develop cell therapeutics using hPSCs to generate and replace somatic cells that are lost...

...Shinichiro Ogawa; James Surapisitchat; Carl Virtanen; Mina Ogawa; Maryam Niapour; Kim S. Sugamori; Shuang Wang; Laura Tamblyn; Chantal Guillemette; Ewa Hoffmann; Bin Zhao; Stephen Strom; Rebecca R. Laposa; Rachel F. Tyndale; Denis M. Grant; Gordon Keller Human pluripotent stem cells (hPSCs...