...Ming-Zhe Dong; Ying-Chun Ouyang; Shi-Cai Gao; Xue-Shan Ma; Yi Hou; Heide Schatten; Zhen-Bo Wang; Qing-Yuan Sun ABSTRACT Mammalian early embryo cells have complex DNA repair mechanisms to maintain genomic integrity, and homologous recombination (HR) plays the main role in response to double-strand...

... lines. Neural stem cell CRISPR/Cas9 Genome editing Gene targeting Epitope tagging Homologous recombination Glioblastoma Transcription factor Targeted editing of endogenous genes via homologous recombination (HR) – termed gene targeting – has been widely deployed in studies...

...Jimann Shin; Jiakun Chen; Lilianna Solnica-Krezel Custom-designed nucleases afford a powerful reverse genetic tool for direct gene disruption and genome modification in vivo . Among various applications of the nucleases, homologous recombination (HR)-mediated genome editing is particularly useful...

...Luis Alberto Baena-Lopez; Cyrille Alexandre; Alice Mitchell; Laurynas Pasakarnis; Jean-Paul Vincent Gene targeting by ‘ends-out’ homologous recombination enables the deletion of genomic sequences and concurrent introduction of exogenous DNA with base-pair precision without sequence constraint...

... transcription factors do not form root hairs. Interestingly, these transcription factors affect only the development of root hairs, because pollen tubes, the other tip-growing cell in seed plants, are not affected ( Menand et al., 2007 ). Box 3. Gene targeting in P. patens Homologous recombination...

... † Present address: Department of Pharmaceutical Sciences, Program in Molecular and Cellular Biology, Oregon State University, Corvallis, OR 9731,USA 30 5 2007 © 2007. 2007 Alopecia Hair cycle Growth factors EGF TPA Wnt pathway Homologous recombination The general...

... targeting Allelic replacement Homologous recombination Morphology evolves through changes in the genetic regulatory networks that govern development ( Carroll,2005 ; Carroll et al.,2005 ; Davidson,2001 ; Stern,2000 ). In principle, regulatory networks and gene expression can evolve...

... Laboratories, 126 Lincoln Avenue, Rahway,NJ 07065-0900, USA † These authors contributed equally to this work ‡ Present address: Hôpital Bichat–Claude-Bernard, 46 Rue Henri-Huchard, 75018 Paris, France 14 9 2005 ©2005. 2005 Homologous recombination Knockout Hypertrophic...

... to be understood (reviewed by Niswander, 2003 ; Tickle, 2003 ). Mouse Limb Anteroposterior patterning Dorsoventral specification Bmp signalling Homologous recombination ©2005. 2005 25 4 2005 ‡ Present address: INSERM U679, Neurobiologie et Thérapeutique Expérimentale...

... and in osteoblasts. Moreover, a mutation in the human MMP13 gene causes the Missouri variant of spondyloepimetaphyseal dysplasia. Inactivation of Mmp13 in mice through homologous recombination led to abnormal skeletal growth plate development. Chondrocytes differentiated normally but their exit from the growth plate...

... GnRH neurons Peripheral nerve Peripheral neuropathy Dysmyelination Homologous recombination Knockout Targeted inactivation Gene targeting COE2 O/E3 Olf/Ebf genes ( Garel et al.,1997 ; Hagman et al.,1993 ; Malgaretti et al.,1997 ; Wang and Reed,1993 ; Wang et al.,1997 ) (reviewed...

... ). Participation of individual brachial somites in skeletal muscles of the avian distal wing . Anat. Embryol. 194 , 327 – 339 . 10.1007/BF00198534 Mouse Homeodomain protein Msx genes Homologous recombination Heterospecific grafts β-galactosidase Differentiation In situ hybridisation Muscle...

... is expressed in inner cell mass cells and the primitive streak, and later is restricted to the developing heart. To investigate the role of Cr1 during mouse development, mice were generated that contain a null mutation of both Cr1 genes, derived from homologous recombination in embryonic stem cells...

...Weiming Xu; Hélène Baribault; Eileen D. Adamson ABSTRACT The vinculin gene codes for a cytoskeletal protein, found in focal adhesion plaques and in cell-cell adherens junctions. Vinculin was inactivated by homologous recombination using a targeting vector in embryonic stem (ES) cells...

... and endocrine cells. * Author for correspondence (e-mail address: panthier@jouy.inra.fr ) 29 06 1996 © 1996 by Company of Biologists 1996 homologous recombination ES cells W locus c- kit proto-oncogene stem cell factor receptor tyrosine kinase mouse W lacZ / W lacZ mouse...

... protein mediates a transcriptional repression via direct binding to specific DNA sequences. To gain further insight into the function of Mel-18, we have inactivated the mel-18 locus by homologous recombination. Mice lacking mel-18 survive to birth and die around 4 weeks after birth after exhibiting strong...

... during mouse embryogenesis, we used the homologous recombination technique in embryonic stem cells and generated Pax7 – / – mice. Homozygous animals are born but die shortly after weaning. They exhibit malformations in facial structures involving the maxilla and nose. Our analysis suggests...

... as myogenesis, and introduce a new lineage marker for the developing brain. 19 09 1995 © 1995 by Company of Biologists 1995 myogenic conversion myf-5 homologous recombination brain development Numerous transcription factors including members of the homeobox ( Krumlauf, 1994...

... into the functional RBP-J к gene in embryonic stem (ES) cells by homologous recombination. Null mutant ES cells survived but null mutant mice showed embryonic lethality before 10.5 days of gestation. The mutant mice showed severe growth retar-dation as early as 8.5 days of gestation. Developmental abnormalities...

.... * Author for correspondence 30 05 1995 © 1995 by Company of Biologists 1995 goosecoid homologous recombination mouse craniofacial defects Originally identified in Xenopus ( Blumberg et al., 1991 ), the goosecoid gene has been shown to be present in many different phyla...