... the same in the mutant MEFs as well. The coverage of the respective histone modifications on the Xa and autosomes as well as that on the Xi was compared between EpiSCs and MEFs ( Fig. S7A ). The coverage of H3K9me3 on the Xi was reduced by one-quarter and one-half of wild-type in mutant EpiSCs and MEFs...

... the major lineage decisions in vivo . By analyzing active and repressive histone modifications, we show that stem cell identity genes are silenced during differentiation by loss of their activating marks and not via repressive histone modifications. Our analysis also uncovers a new set of genes specifically...

... development remain difficult to capture. We applied a Fab-based live endogenous modification labeling technique to monitor the changes in histone modification levels during zygotic genome activation (ZGA) in living zebrafish embryos. Among various histone modifications, H3 Lys27 acetylation (H3K27ac...

... measurements of chromatin modifications, describing how these methods can be used to connect chromatin and gene expression, classify cell types, and study development and disease. DNA methylation Chromatin regulation Gene regulation Histone modifications Single cell detection National...

... methylation and repressive histone modifications. Here we follow the gain of imprinted expression of Igf2r during in vitro ES cell differentiation and show that it coincides with the onset of paternal-specific expression of the Airn ncRNA. Notably, although Airn ncRNA expression leads, as predicted, to gain...

... cellular dynamics ensures successful implementation of genome-wide epigenetic reprogramming in migrating PGCs. 10 5 2007 © 2007. 2007 Primordial germ cells (PGCs) Histone modifications Reprogramming Cell cycle Epigenetics Mouse In the development of multicellular organisms...

... by antagonizing Su(var)3-9-mediated heterochromatization. * Author for correspondence (e-mail: [email protected] ) 7 11 2005 © 2006. 2006 JIL-1 kinase Chromatin Histone modifications HP1 Drosophila Higher order chromatin structure is important for epigenetic...

... a striking reduction of histone modifications that correlate with transcriptional activation when compared with the genome as a whole. The histone modification spectrum on the X chromosome corresponds with a lack of transcriptional competence, as measured by reporter transgene arrays. The X chromosome in XO...