... signaling is a key regulator of stem cell properties in the developing and postnatal brain, and Hes1 is a major effector that strongly inhibits neuronal differentiation and maintains NSCs. To manipulate Hes1 expression levels in NSCs, we generated transgenic (Tg) mice using the Tet-On system. In Hes1...

...Shohei Ochi; Yui Imaizumi; Hiromi Shimojo; Hitoshi Miyachi; Ryoichiro Kageyama ABSTRACT The expression of the transcriptional repressor Hes1 oscillates in many cell types, including neural progenitor cells (NPCs), but the significance of Hes1 oscillations in development is not fully understood...

...Mette C. Jørgensen; Kristian H. de Lichtenberg; Caitlin A. Collin; Rasmus Klinck; Jeppe H. Ekberg; Maja S. Engelstoft; Heiko Lickert; Palle Serup ABSTRACT Mutations in Hes1 , a target gene of the Notch signalling pathway, lead to ectopic pancreas by a poorly described mechanism. Here, we use...

... induces expression of the Ngn3 repressor Hes1. At the transition from high to intermediate Notch activity, only Sox9, but not Hes1, is maintained, thus de-repressing Ngn3 and initiating endocrine differentiation. In the absence of Sox9 activity, endocrine and ductal cells fail to differentiate, resulting...

...Jonas Ahnfelt-Rønne; Mette C. Jørgensen; Rasmus Klinck; Jan N. Jensen; Ernst-Martin Füchtbauer; Tye Deering; Raymond J. MacDonald; Chris V. E. Wright; Ole D. Madsen; Palle Serup Neurog3-induced Dll1 expression in pancreatic endocrine progenitors ostensibly activates Hes1 expression via Notch...

... The authors declare no competing financial interests. 13 5 2011 © 2011. Hedgehog Intestine Stomach Endoderm Mesoderm Notch Hes1 Mouse The union of gut endoderm and splanchnic mesoderm generates the digestive tract and its derivatives – the liver, pancreas...

..., Notch and its target gene Hes1 are thought to inhibit endocrine and exocrine specification. Although differentiated cells appear to downregulate Hes1 , it is unknown whether Hes1 expression marks multipotent progenitors, or else lineage-restricted precursors. Moreover, although rare cells of the adult...

... allocation. Finally, Hes1 was identified as an RBPjκ-dependent Notch target gene important for MPC maintenance and the suppression of in vitro chondrogenesis. Several reports have indicated that Notch signaling components are expressed during early vertebrate limb development. These data have analyzed...

... to be a downstream target of Alk5 in vivo and in vitro. Loss of Alk5 -mediated signaling also stimulated Pten gene expression and inhibited ERK phosphorylation in vivo. Using lung epithelial cells, we show that Alk5 -regulated Hes1 expression is stimulated through Pten and the MEK/ERK and PI3K/AKT pathways. Thus...

... © 2008. 2008 Notch Stem cells Leydig cell Germ cell Testis cord Hes1 Mouse Mouse embryonic gonad development begins at ∼10.0 days post coitum(dpc) with the thickening of the epithelial layer (the coelomic epithelium)overlying the mesonephric tubules ( Brennan and Capel, 2004...

... tissue has not yet been determined. Using mouse embryos and lineage-tracing analysis, we found that the prospective choroid plexus region initially gives rise to Cajal-Retzius cells, specialized neurons that guide neuronal migration. Inactivation of the bHLH repressor genes Hes1,Hes3 and Hes5 upregulated...

... homologs of achaete-scute complex ( Mash1 ) ( Ascl1 – Mouse Genome Informatics); hairy and enhancer of split1 ( Hes1 ); and the expression of Notch/Notch-ligand system in the fetal and adult mouse lungs, and in the lungs of Mash1 - or Hes1 -deficient mice. Immunohistochemical studies revealed that Mash1...

..., unlike Hes1 , which is expressed only by the former cells. Hes6 alone does not bind to the DNA but suppresses Hes1 from repressing transcription. In addition, Hes6 suppresses Hes1 from inhibiting Mash1-E47 heterodimer and thereby enables Mash1 and E47 to upregulate transcription in the presence of Hes1...