... of HSPGs in regulating distinct vertebrate Fgfs. * Author for correspondence (e-mail: [email protected]) 9 9 2005 ©2005. 2005 Zebrafish HSPG Fgf10 Limb development Ext Heparan Heparin The development of metazoan animals is directed by cell-cell...

...Bibhash C. Paria; Klaus Elenius; Michael Klagsbrun; Sudhansu K. Dey Blastocyst implantation requires molecular and cellular interactions between the uterine luminal epithelium and blastocyst trophectoderm. We have previously shown that heparin-binding EGF-like growth factor (HB-EGF) is induced...

...-dependent Ca 2+ influx, suppressed the initial [Ca 2+ ] i transient but did not affect the following two phases of [Ca 2+ ] i changes. Injection of heparin, an antagonist of the inositol 1,4,5-trisphosphate (IP 3) receptor, inhibited the later two phases without much affecting the initial transient...

... proteoglycan that mediates agrin activity. To further test this hypothesis, we have compared the ability of differentially active agrin isoforms to interact with a model component of proteoglycans, heparin, as well as with the putative proteoglycan α-dystroglycan. We demonstrate that an alternately spliced...

...Victor Nurcombe; Neil Fraser; Ellen Herlaar; John K. Heath ABSTRACT MK is a gene encoding a secreted heparin-binding polypeptide originally isolated by differential screening for genes induced by retinoic acid (RA) in HM-1 embryonal carcinoma cells. Here we report that MK is expressed at high...

...Marie C. Defrances; Helmut K. Wolf; George K. Michalopoulos; Reza Zarnegar ABSTRACT Hepatocyte growth factor (HGF), a heparin-binding polypeptide mitogen, stimulates DNA synthesis in adult rat and human hepatocytes and in several other cells of epithelial origin. Recently, it was determined...

... of lower specificity do inhibit the transfilter induction: heparin and suramin. Suramin will also inhibit induction in animal-vegetal combinations with no intervening membranes while heparin does not. This suggests that the heparin inhibition can only occur when there is a liquid gap between the tissues...

... to early chick blastoderms grown in vitro under defined culture conditions (DCM). Both heparin and suramin were capable of blocking the formation of mesodermal structures in a dose-dependent manner. Our results indicate that FGF-like substances may need to be present for axial structures to develop...

...Shohei Mitani ABSTRACT Gastrulation was examined in Xenopus embryos injected with various polysaccharides into the blastocoel cavity. The progression of gastrulation was assessed by observing pigmentation and yolk plug size in vegetative view embryos. In heparin-or dextran-sulphate-injected embryos...

...John K. Heath; Gary D. Paterno; A. Catherine Lindon; Dylan R. Edwards ABSTRACT Culture medium conditioned by P19 embryonal carcinoma (EC) cells contains potent mitogenic activity which is markedly potentiated when the medium is conditioned in the presence of heparin. Fractionation of P19 medium...