...Rebecca K. Schiavo; Owen J. Tamplin ABSTRACT Hematopoietic stem and progenitor cells (HSPCs) are multipotent cells that self-renew or differentiate to establish the entire blood hierarchy. HSPCs arise from the hemogenic endothelium of the dorsal aorta (DA) during development in a process called...

...Michael Lie-A-Ling; Elli Marinopoulou; Andrew J. Lilly; Mairi Challinor; Rahima Patel; Christophe Lancrin; Valerie Kouskoff; Georges Lacaud ABSTRACT During ontogeny, hematopoietic stem and progenitor cells arise from hemogenic endothelium through an endothelial-to-hematopoietic transition...

...Amanda D. Yzaguirre; Elizabeth D. Howell; Yan Li; Zijing Liu; Nancy A. Speck ABSTRACT Hematopoietic cells differentiate during embryogenesis from a population of endothelial cells called hemogenic endothelium (HE) in a process called the endothelial-to-hematopoietic transition (EHT...

... 2018. Published by The Company of Biologists Ltd 2018 Summary: This Review gives an overview of what is known regarding niche heterogeneity at distinct stages of HSC ontogeny, from embryonic life to the adult bone marrow. Bone marrow Hematopoietic stem cell Hemogenic endothelium...

... transcription. Although the factors regulating EHT in hemogenic endothelium (HE) of the dorsal aorta have been relatively well studied, the molecular regulation of yolk sac HE remains poorly understood. Here, we show that SOX7 inhibits the expression of RUNX1 target genes in HE, while having no effect on RUNX1...

...Laurent Yvernogeau; Rodolphe Gautier; Hanane Khoury; Sara Menegatti; Melanie Schmidt; Jean-Francois Gilles; Thierry Jaffredo Adult-type hematopoietic stem and progenitor cells are formed during ontogeny from a specialized subset of endothelium, termed the hemogenic endothelium, via an endothelial...

... studies have suggested that HSC specification begins during the convergence of posterior lateral plate mesoderm (PLM), well before aorta formation and runx1 transcription. Further understanding of the earliest stages of HSC specification necessitates an earlier marker of hemogenic endothelium. Studies...

..., such as the ‘embryonic mesoangioblasts’, originate in vitro from the endothelium. Using a genetic lineage tracing approach, we show that early extra-embryonic endothelium generates, in a narrow time-window and prior to the hemogenic endothelium in the major embryonic arteries, hematopoietic cells that migrate...

... potential, whereas scl-α is expressed later in nascent HSCs as they egress from VAE cells. In accordance with their expression, loss-of-function studies coupled with in vivo imaging analysis reveal that scl-β acts earlier to specify hemogenic endothelium, which is later transformed by runx1 into HSCs. Our...

...Joanna Tober; Amanda D. Yzaguirre; Eileen Piwarzyk; Nancy A. Speck The transcription factor Runx1 is essential for the formation of yolk sac-derived erythroid/myeloid progenitors (EMPs) and hematopoietic stem cells (HSCs) from hemogenic endothelium during embryogenesis. However, long-term...

... of culture (days 2-4; Fig. 2A,D , Mi+ALKi). * Author for correspondence ( wong@jhmi.edu ) Competing interests statement The authors declare no competing financial interests. 18 4 2011 © 2011. 2011 Hemangioblast Hemogenic endothelium Serum-free differentiation Single...