... of mesodermal progenitors to hematopoiesis. Gastrulation Hemangioblast Mesoderm At the early stages of embryonic development, the multipotent epiblast gives rise to the three germ layers which are subsequently specified to form all tissues of the developing organism ( Tam and Loebel, 2007...

... of culture (days 2-4; Fig. 2A,D , Mi+ALKi). * Author for correspondence ( wong@jhmi.edu ) Competing interests statement The authors declare no competing financial interests. 18 4 2011 © 2011. 2011 Hemangioblast Hemogenic endothelium Serum-free differentiation Single...

... muscle cell Blood cell Endothelial cell Notch BMP Wnt dHAND Scl Tal1 Hemangioblast Progenitor During primitive hematopoiesis, blood cells (BCs) and endothelial cells(ECs) are generated in the extraembryonic mesoderm from a common pool of progenitors ( Baron, 2003 ; Lugus et al., 2005...

... the embryonic stem cell differentiation system to investigate the regulation of primitive erythroid development at the level of the hemangioblast. We show that the combination of Wnt signaling with inhibition of the Notch pathway is required for the development of this lineage. Inhibition of Notch signaling...

... the selective and efficient differentiation of mouse ES cells to hematopoiesis. Each of these factors appears to regulate a step of the process: Bmp4 promotes the very efficient formation of mesoderm; bFGF and activin A induce the differentiation of these mesodermal precursors to the hemangioblast fate...

... of the hemangioblast, an embryonic progenitor of the hematopoietic and endothelial lineages. Eng -/- , Eng +/- and Eng +/+ mouse embryonic stem (ES) cells were differentiated as embryoid bodies (EBs) and assayed for blast colony-forming cells (BL-CFCs). Our results showed a profound reduction in hemangioblast...

... mechanisms, we compared gene expression profiles between embryonic stem (ES) cell-derived hemangioblasts (Blast-Colony-Forming Cells, BL-CFCs)and their differentiated progeny, Blast cells. Bioinformatic analysis indicated that BL-CFCs resembled other stem cell populations. A role for Gata2 , one of the BL...

...Chie Furuta; Hideo Ema; Shin-ichiro Takayanagi; Takunori Ogaeri; Daiji Okamura; Yasuhisa Matsui; Hiromitsu Nakauchi Vasculogenesis and hematopoiesis are thought to arise in hemangioblasts,the common progenitors of cells in vessels and in blood. This scheme was challenged by kinetic analysis...

... in blood cell and vessel development. We demonstrate that hypoxia accelerates the expression of Brachyury (a mesoderm-specific transcription factor), BMP4 (a mesoderm-promoting growth factor) and FLK1 (a marker of hemangioblasts, the bipotential progenitor of endothelial and hematopoietic cells...

...Hans Jörg Fehling; Georges Lacaud; Atsushi Kubo; Marion Kennedy; Scott Robertson; Gordon Keller; Valerie Kouskoff The hematopoietic and endothelial lineages derive from mesoderm and are thought to develop through the maturation of a common progenitor, the hemangioblast. To investigate...

...Yun Shin Chung; Wen Jie Zhang; Elizabeth Arentson; Paul D. Kingsley; James Palis; Kyunghee Choi Accumulating studies support the idea that a common progenitor, termed the hemangioblast, generates both hematopoietic and endothelial cell lineages. To better define the relationship between these cell...

... hematopoietic precursors. The block in definitive hematopoiesis is similar to that observed in Runx1 knockout mice, implying that zebrafish Runx1 has a function equivalent to that in mammals. Our data suggest that zebrafish Runx1 functions in both blood and vessel development at the hemangioblast level...

...Wayne Liao; Chi-Yip Ho; Yi Lin Yan; John Postlethwait; Didier Y. R. Stainier ABSTRACT During embryogenesis, endothelial and blood precursors are hypothesized to arise from a common progenitor, the hemangioblast. Several genes that affect the differentiation of, or are expressed early in, both...

... and endothelial cells, the hemangioblast. Access to this initial cell population committed to the hematopoietic lineage provides a unique opportunity to characterize hematopoietic commitment events. Here, we show that BL-CFC expresses the receptor tyrosine kinase, Flk1, and thus we took advantage of the BL-CFC...

...), hemangioblasts are formed much more abundantly in flt-1 − / − embryos. This increase is primarily due to an alteration in cell fate determination among mesenchymal cells, rather than to increased proliferation, migration or reduced apoptosis of flt-1 − / − hemangioblasts. We further show that the increased...

..., a Flk-1 ligand, determine whether putative hemangioblasts differentiate into cells of the hematopoietic or endothelial lineage in culture ( Eichmann et al., 1997 ). Studies of chimeras formed between flk - 1 −/− embryonic stem cells and wild-type mouse blastocysts show that Flk-1 is required cell...

.... This work was supported by grants from the Ministry of Education, Science, Sports and Culture of Japan (nos 07CE2005, 06277102 and 06NP1101). Though it is not clear whether the FLK1 + VE-cadherin ™ population represents only the multipotent mesoderm or includes the bipotent hemangioblast, our single...

... a transient cell population that develops early and is lost quickly during embryoid body development. These findings provide strong evidence that the blast colony-forming cell represents the long-hypothesized hemangioblast, the common precursor of the hematopoietic and endothelial lineages. * Author...