... and Ripply1, which are known regulators of somite boundary formation, also control spatiotemporal aspects of skeletal myogenesis in zebrafish embryos. Dermomyotome Myotome Segmentation Maturation Zebrafish Heat shock Protein degradation Determination front Wavefront Gene regulatory network...

.../47 mex-3 mutant embryos examined expressed vab-7::lacZ ectopically. An embryo of genotype mex-3(zu155) dpy-5(e61); eIs24 . was lineaged and then fixed and heat shock of eIs24 . Nearly all pre-morphogenesis embryos contained ectopic β-galactosidase after heat shock, but heat-shocked embryos...

... is located in the Golgi apparatus, which has a different morphology to that seen in COS cells. 03 05 1994 © 1994 by Company of Biologists 1994 argos Drosophila eye development secreted protein determination heat shock The interaction between cells plays an important...

...Acaimo González-Reyes; Ana Macías; Ginés Morata ABSTRACT Previously published experiments have shown that the endogenous Dfd gene can be ectopically activated by its own (heat-shock-driven) product in a subset of cells of different segments. This results in the differentiation of maxillary...

...Nick Ovsenek; John J. Heikkila ABSTRACT We have examined the activity of the Xenopus heat-shock transcription factor (HSF) in extracts from stressed and unstressed embryos at various stages of development using DNA mobility shift analysis. A specific interaction between HSF and a synthetic...

... genomic clone was detected in heat-shocked gastrula stage embryos (lane 6). RNase protection studies have shown that the endogenous hsp 30 genes are not expressed at this stage; however, it was possible that the injected genes may somehow activate the expression of the endogenous hsp 30 genes. This does...

...Rashmi Kothary; Susan Clapoff; Susan Darling; Marc D. Perry; Laurence A. Moran; Janet Rossant ABSTRACT Transgenic mice have been generated that express the E. coli β-galactosidase gene under the control of the promoter from the mouse heat-shock gene, hsp68 . Sequences from -664 to +113 relative...

...Robin E. Davis; Mary Lou King ABSTRACT We have investigated the Xenopus heat-shock response of somatic cells, oocytes and embryos. Xenopus defolli-culated oocytes displayed a highly variable response to heat shock depending on the culture medium. Intact follicles, however, respond to heat stress...

...D. R. N. Primmett; C. D. Stern; R. J. Keynes ABSTRACT A single heat shock, given to 2-day-old chick embryos, can generate multiple but discrete somite and skeletal anomalies. Each of these anomalies is restricted to one, or at the most two, consecutive segments. The anomalies are separated from...

...Jean-Claude Beetschen; Jean Gautier ABSTRACT Axolotl eggs were heat shocked (36·8°C, 10min) inside their jelly layers. Heat shock (HS) was shown to induce the precocious appearance of a grey crescent (GC) in a number of eggs immediately after fertilization ( Benford & Namenwirth, 1974...

... of heat-shock protein (HSP-70) gene expression. * Current address: The Proctor and Gamble Company, Miami Valley Laboratories, PO Box 39175, Cincinnati, OH 45247, USA 11 03 1987 © 1987 by Company of Biologists 1987 Yu et al . (1985) recently showed that in vitro exposure...

... uniformity indicates good timekeeping. Timekeeping is not impaired among the survivors following heat shocks that retard development and disturb and curtail morphogenesis. The immediate effect of heat shock is a stoppage of development, the duration of which is the same for all embryos in the same treatment...

...Jane E. Mee; Vernon French ABSTRACT A heat shock (of 15 min at 48°C) given to early embryos of the locust, Schistocerca gregaria , results in localized abnormalities in the segment pattern subsequently formed. Most defects involve two consecutive segments of the thorax or abdomen...

...Jane E. Mee; Vernon French ABSTRACT The effect of heat shock (15 min at 48 °C) on segmentation has been investigated in the short germ embryo of the locust (Schistocerca gregaria) . Prior to formation of the germ anlage and at the disc stage heat shock considerably reduced the survival of eggs...