... antagonistic interaction between the GLI3 repressor and HAND2 results in distinct and non-overlapping anterior-distal Gli3 and posterior Hand2 expression domains. This is a hallmark of the establishment of antero-posterior limb axis polarity, together with spatially restricted expression of homeodomain...

..., we identified Gli3 as a global regulator of superstructure patterning, whereas Pbx1 , Pbx2 , Hoxa11 and Hoxd11 act as proximal and distal regulators, respectively. Moreover, by demonstrating a dose-dependent pattern regulation in Gli3 and Pbx1 compound mutations, we show that the global and regional...

... these neurons develop at the correct time and place and in accurate numbers constitutes a major challenge. Here, we demonstrate a novel role for Gli3, a key regulator of cortical development, in cortical neurogenesis. We show that the onset of neuron formation is delayed in Gli3 conditional mouse mutants. Gene...

... embryos, revealed extensive downregulation of Sonic hedgehog (Shh) pathway components: patched receptors, Hedgehog interacting protein and the transcriptional activator Gli1. By contrast, Gli3 , a transcriptional repressor, was de-repressed and confirmed as a direct miR-133 target. Phenotypically, miR-133...

... reduced Hedgehog signaling between Smo and Sufu, expanded brain size and polydactyly. Dzip1l Chibby Bromi Cilia Hedgehog Gli2 Gli3 Mouse National Institutes of Health 10.13039/100000002 R01GM114429 R01HL107493 National Natural Science Foundation of China 10.13039...

...Anisha Solanki; Diana C. Yanez; Susan Ross; Ching-In Lau; Eleftheria Papaioannou; Jiawei Li; José Ignacio Saldaña; Tessa Crompton ABSTRACT Gli3 is a Hedgehog (Hh)-responsive transcription factor that can function as a transcriptional repressor or activator. We show that Gli3 activity in mouse...

... equally to this work Competing interests statement The authors declare no competing financial interests. 4 10 2011 © 2011. Smoothened V1 V2 Floor plate Gli3 Interneuron Zebrafish In mouse, Hedgehog (Hh) signalling is required for the formation...

...) or suppressor of fused (Sufu). Genetic analysis shows that although PKA is important for production of the repressor form of Gli3, the principal function of PKA in the Shh pathway in neural development is to restrain activation of Gli2. Activation of the Hh pathway in PKA mutants depends on cilia...

... of gestation. Investigating the developmental origin of this defect, we have established that E12.5 Ftm −/− telencephalic neuroepithelial cells lack primary cilia. Moreover, in the anterior telencephalon, the subpallium is expanded at the expense of the pallium, a phenotype reminiscent of Gli3 mutants...

...Chengbing Wang; Yong Pan; Baolin Wang Gli2 and Gli3 are primary transcriptional regulators that mediate hedgehog (Hh) signaling. Mechanisms that stabilize and destabilize Gli2 and Gli3 are essential for the proteins to promptly respond to Hh signaling or to be inactivated following the activation...

... not been provided. Moreover, the extent to which different Gli proteins contribute to Ihh functions is not fully understood. Previous work has shown that de-repression of the Gli3 repressor is the predominant mode through which Ihh controls chondrocyte proliferation and maturation, but that osteoblast...

... that C2cd3 is an essential regulator of intracellular transduction of the Hedgehog signal. Furthering a link between Hedgehog signaling and cilia function, we find that cilia formation and proteolytic processing of Gli3 are disrupted in C2cd3 mutants. Finally, we observe C2cd3 protein at the basal body...

... in the expansion of ventrally located progenitors. Finally,we show genetically that Wnt signaling interacts with Hh signaling at least in part through regulating the transcription of Gli3 . Our results reveal a novel mechanism by which ventral patterning is achieved through a coordination of Wnt and Shh signaling...

... questioned because loss of the Shh gene has little effect on ventral development if the Gli3 gene is also mutant. Consequently, another ventral determinant must exist. Here, genetic evidence establishes that FGFs are required for ventral telencephalon development. First, simultaneous deletion of Fgfr1...

... for mammary line and placode formation. The mammary line is displaced dorsally, and formation of placode 3 is impaired in Pax3 ILZ/ILZ mutants, which do not form ventral somitic buds. Mammary line formation is impaired and placode 3 is absent in Gli3 Xt-J/Xt-J and hypomorphic Fgf10 mutants, in which...

... University School of Medicine, New Haven, CT 06536, USA 8 3 2006 © 2006. 2006 Gli1 Gli2 Gli3 Shh Cerebellum Patterning Mouse The relatively few well-defined cell types within the cerebellum and the stereotypical foliation pattern make the cerebellum...

...Ming Chang Hu; Rong Mo; Sita Bhella; Christopher W. Wilson; Pao-Tien Chuang; Chi-chung Hui; Norman D. Rosenblum Truncating mutations in Gli3 , an intracellular effector in the SHH-SMO-GLI signaling pathway, cause renal aplasia/dysplasia in humans and mice. Yet, the pathogenic mechanisms...

... the onset of hypertrophic differentiation, and it regulates chondrocyte proliferation and ossification of the perichondrium independently of PTHrP. To investigate how the Ihh signal is translated in the different target tissues, we analyzed the role of the zinc-finger transcription factor Gli3, which acts...

... transcription factors are key effectors of hedgehog signaling, it has not been established which Gli protein mediates Ihh activity in skeletal development. Here, we show that removal of Gli3 in Ihh -null mouse embryos restored normal proliferation and maturation of chondrocytes,but only partially rescued...

... and Gli3 , genes previously implicated in anteroposterior pre-patterning of the limb-forming region, are also involved in refining the position of the limbs. Our data suggest a new role for Tbx3 in positioning the limb along the main body axis through a genetic interplay between dHand and Gli3...