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Keywords: Germ layer
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Journal Articles
Journal: Development
Development (2019) 146 (21): dev174938.
Published: 4 November 2019
... have mainly involved systems for tracing a single gene, which are limited in their ability to facilitate direct comparisons of the contributions of different cell lineages. We have developed a new combinatorial system for tracing all three germ layers using self-cleaving 2A peptides and multiple site...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (5): 897–904.
Published: 1 March 2017
...Masumi Tokuhisa; Miyuki Muto; Hiroki Nishida The animal-vegetal (A-V) axis is already set in unfertilized eggs. It plays crucial roles in coordinating germ-layer formation. However, how the A-V axis is set has not been well studied. In ascidians, unfertilized eggs are already polarized along...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2013) 140 (5): 976–986.
Published: 1 March 2013
... germ layer specification, and that this early function is probably independent of the recognised role of Lin28 proteins in negatively regulating let-7 miRNA biogenesis. Total RNA was isolated using miRVana miRNA Isolation Kit (Applied Biosystems). cDNA was synthesized with miRNA-specific primers...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2004) 131 (19): 4687–4696.
Published: 1 October 2004
...Fiona C. Wardle; James C. Smith During blastula and gastrula stages of Xenopus development, cells become progressively and asynchronously committed to a particular germ layer. We have analysed the expression of genes normally expressed in ectoderm,mesoderm or endoderm in individual cells from early...
Journal Articles
Journal: Development
Development (2003) 130 (12): 2695–2704.
Published: 15 June 2003
...Douglas W. Houston; Christopher Wylie One of the earliest lineage restriction events in embryogenesis is the specification of the primary germ layers: ectoderm, mesoderm and endoderm. In Xenopus , germ layer specification occurs prior to gastrulation and requires the transcription factor VegT both...