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Keywords: Genome engineering
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Journal Articles
Journal: Development
Development (2023) 150 (4): dev201198.
Published: 14 February 2023
...-specific labelling and genome engineering in vivo . Cre-loxP , Flp-FRT and Dre-rox are three known systems that mediate SSR events in genomic DNA. An intersectional approach results in transgene expression that is more cell type-specific by combining different recombination systems...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2014) 141 (21): 4042–4054.
Published: 1 November 2014
...Ying Peng; Karl J. Clark; Jarryd M. Campbell; Magdalena R. Panetta; Yi Guo; Stephen C. Ekker Recent advances in the targeted modification of complex eukaryotic genomes have unlocked a new era of genome engineering. From the pioneering work using zinc-finger nucleases (ZFNs), to the advent...
Journal Articles
Journal: Development
Development (2014) 141 (19): 3807–3818.
Published: 1 October 2014
... in determining the efficiency of HR-mediated genome engineering. Implementing these modifications improved the efficiency of zebrafish knock-in generation, with over 10% of the injected F0 animals transmitting gene-targeting events through their germline. We generated two HR-mediated insertion alleles of sox2...
Includes: Supplementary data