... understanding of the genetic basis of developmental processes. However, the generalizability of these findings cannot be confirmed without functional genetic analyses in additional organisms. Direct genome editing using targeted nucleases has the potential to transform hitherto poorly understood organisms...

... roles for AKT1, a serine/threonine kinase, in placental development using a genome-edited/loss-of-function rat model. Disruption of AKT1 resulted in placental, fetal and postnatal growth restriction. Forkhead box O4 ( Foxo4 ), which encodes a transcription factor and known AKT substrate, was abundantly...

...). Crispr Maternal effect Genome editing Early development Zebrafish Kpna7 Fhdc3 Summary: A method exploiting the biallelic editing ability CRISPR-Cas9 to create maternal crispants enables the identification of maternal-effect genes in a single generation. © 2021. Published...

... of subsequent effects on cell behavior. Here, we present a single-plasmid approach for genome editing in chick embryos to study experimentally perturbed cells in an otherwise normal embryonic environment. To achieve this, we have engineered a plasmid that encodes Cas9 protein, gene-specific guide RNA (gRNA...

... effects and that tek is missing in most fish. Tek ( Tie2 ) Cardiovascular development Genome editing Morpholino Evolutionary genetics Functional diversification The cardiovascular system is one of the first organs to form and function, and its development is regulated by complex...

... enhancer elements near these target genes. Functional experiments performed in vitro and in vivo confirm that this network of genes requires ERK, ERG and p300 activity. Finally, genome-editing and transgenic approaches demonstrate that a highly conserved ERG-bound enhancer located upstream of HLX (which...

... ); and (4) engineering of glioma mutations ( TP53 deletion; H3F3A point mutations). These resources and optimised methods enable facile and scalable genome editing in mammalian NSCs, providing significant new opportunities for functional genetic analysis. * Author for correspondence ( steven.pollard...

... technological advances in single-cell analysis, allowing in-depth analysis of the human embryo. This field could shortly gain more momentum as novel genome editing technologies might, for the first time, also allow functional genetic studies in the human embryo. In this Spotlight article, we summarize...

... Stem Cell Symposium this year. Bioinformatics Computational biology Epigenetics Genome editing Stem cells Transcription Cancer Research UK 10.13039/501100000289 A19778 Quantitative approaches have been boosted across many areas of biology by the relentless improvements...

...Ira L. Blitz; Margaret B. Fish; Ken W. Y. Cho CRISPR/Cas9 genome editing is revolutionizing genetic loss-of-function analysis but technical limitations remain that slow progress when creating mutant lines. First, in conventional genetic breeding schemes, mosaic founder animals carrying mutant...

.... Using CRISPR/Cas9-mediated genome editing, we generated and analyzed developmental defects in mice carrying additional Golgb1 loss-of-function mutations, which supported a crucial requirement for Golgb1 in palate development. Through maxillary explant culture assays, we demonstrate that the Golgb1...

... Mutagenesis Genome editing RNP CrispantCal CrispRVariants Cas9 nuclease-mediated mutagenesis through non-homologous end joining (NHEJ) repair enables rapid, site-directed mutagenesis of candidate genes in zebrafish for somatic as well as stable germline mutant analysis ( Chang et al., 2013...

...Uwe Irion; Jana Krauss; Christiane Nüsslein-Volhard The introduction of engineered site-specific DNA endonucleases has brought precise genome editing in many model organisms and human cells into the realm of possibility. In zebrafish, loss-of-function alleles have been successfully produced...

... ( [email protected] ; [email protected] ) Competing interests The authors declare no competing financial interests. 18 6 2014 1 9 2014 © 2014. Published by The Company of Biologists Ltd 2014 Ascidians CRISPR/Cas9 Ciona Ebf Genome editing Islet Recent advances have...

... the activation of the Shh response ( Corbit et al., 2005 ; Huangfu and Anderson, 2005 ; Rohatgi et al., 2007 ). This model explains the widespread activation of the Shh response observed in the absence of Ptch1 ( Goodrich et al., 1997 ). Patched1 Patched2 Shh Genome editing Mouse embryonic stem...

... nucleases (TALENs), which require either complex design and selection or laborious construction. Thus, easy and efficient genome editing tools are still highly desirable for this species. Here, we report that RNA-guided Cas9 nuclease resulted in precise targeted gene disruption in all ten X. tropicalis...

...Alexander Hruscha; Peter Krawitz; Alexandra Rechenberg; Verena Heinrich; Jochen Hecht; Christian Haass; Bettina Schmid Gene modifications in animal models have been greatly facilitated through the application of targeted genome editing tools. The prokaryotic CRISPR/Cas9 type II genome editing...