... compatible with targeted genetic manipulations. However, gene targeting, which is crucial for functional studies of embryonic stem cells, has not been exploited to date in NSC lines. Here, we deploy CRISPR/Cas9 technology to demonstrate a variety of sophisticated genetic modifications via gene targeting...

... The authors declare no competing or financial interests. 6 11 2015 9 3 2016 © 2016. Published by The Company of Biologists Ltd 2016 Summary: A new transgenic mouse line allows sebocyte-specific gene targeting and investigation of the importance of sebaceous lipids in the skin...

...Nicholas Treen; Keita Yoshida; Tetsushi Sakuma; Haruka Sasaki; Narudo Kawai; Takashi Yamamoto; Yasunori Sasakura Custom designed nucleases can simplify gene targeting experiments and have the potential to allow these techniques to be performed in a wide range of organisms. Transcriptional activator...

...Luis Alberto Baena-Lopez; Cyrille Alexandre; Alice Mitchell; Laurynas Pasakarnis; Jean-Paul Vincent Gene targeting by ‘ends-out’ homologous recombination enables the deletion of genomic sequences and concurrent introduction of exogenous DNA with base-pair precision without sequence constraint...

... ) Competing interests statement The authors declare no competing financial interests. © 2013. Published by The Company of Biologists Ltd 2013 Human pluripotent stem cells Directed differentiation Gene targeting High-throughput screening Human pluripotent stem cells (hPSCs), which...

... tissues. X-inactivation Xist Tsix Gene targeting Mouse embryo © 2009. 2009 29 10 2008 * These authors contributed equally to this work † Author for correspondence (e-mail: [email protected] ) For allelic expression analysis of X-linked genes...

... of such regionalised factors. † Authors for correspondence (e-mails: [email protected] ; [email protected] ) * These authors contributed equally to this work 30 7 2008 © 2008. 2008 Gene targeting Teashirt 3 (Tshz3) UPJO Ureter Smooth muscle Diverse viscera...

..., 1030 Vienna, Austria 12 10 2007 © 2008. 2008 X inactivation Antisense regulation Chromatin modification Gene targeting Mouse embryos An unexpected new view of the function of RNA involved in many biological phenomena has emerged from recent advances in transcriptome...

...-cadherin in the basement membrane of frozen sections (E,F,I,J) tended to be more intense than that in paraffin sections (C,D,G,H). hg, hindgut; mn, metanephros; ur, ureter;Wd, Wolffian duct. Scale bars: 100 μm in A,B; 10 μm in C-J. We performed gene targeting of Dlgh1 . Functional Dlgh1...

.... The general architecture of the kidney was largely unaffected in the Cv2-/- mutant(M). The kidney defect phenotype of the Cv2 mutant appears consistent with the idea that Cv2 plays a positive regulatory role in Bmp signaling. However, previous gene targeting studies have demonstrated...

... ) 19 1 2006 © 2006. 2006 PAR3 (PARD3) Gene targeting Epithelial cell polarity Epithelial cyst Heart development Epicardium Mouse The organization of polarized epithelial cells is crucial for ontogeny and function of various tissues and organs in metazoans...

... allele, these four changes were retained, while the I-Sce I site was not( Fig. 1 ). The I-Sce I insertion also introduced a novel Xba I site in conjunction with existing genomic sequence. The resulting 10,893 bp modified fragment of Ubx was subcloned into the gene targeting vector pTV2( Rong et al...

.... 2004 Ccm1 Gene targeting Mice Angiogenesis Stroke Epilepsy Notch Efnb2 Stroke is an important cause of morbidity and mortality in children where it ranks in the top 10 causes of death( Arias and Smith, 2003 ; Lynch et al., 2002 ). Approximately 20% of pediatric stroke cases are due...

... increases IGF bioavailability and mitogenic effectiveness in vitro through regulated cleavage of IGF-binding protein 4 (IGFBP4). To determine its function in vivo, we generated PAPPA-null mice by gene targeting. Mice homozygous for targeted disruption of the PAPPA gene were viable but 60% the size of wild...

... GnRH neurons Peripheral nerve Peripheral neuropathy Dysmyelination Homologous recombination Knockout Targeted inactivation Gene targeting COE2 O/E3 Olf/Ebf genes ( Garel et al.,1997 ; Hagman et al.,1993 ; Malgaretti et al.,1997 ; Wang and Reed,1993 ; Wang et al.,1997 ) (reviewed...

... strains by mating to an early deleter strain ( Lallemand et al., 1998 ). *Author for correspondence (e-mail: [email protected] ) 17 5 2002 © 2002. 2002 Transcriptional alternatives FGF Endochondral ossification Craniosynostosis Gene targeting Mouse Three...

[email protected] ) * These authors contributed equally to this paper 04 10 2000 © 1995 by Company of Biologists 1995 Axis specification Frizzled Gene targeting Mouse Wnt Wnt proteins form one of the major families of secreted ligands that play key roles in developmental...

... ). Gene targeting reveals a critical role for neurturin in the development and maintenance of enteric, sensory, and parasympathetic neurons . Neuron 22 , 253 – 263 . 10.1016/S0896-6273(00)81087-9 Heuckeroth , R. O. , Lampe , P. A. , Johnson , E. M. and Milbrandt , J...

... development, the gene was inactivated by gene targeting. Foetuses lacking Fra1 were severely growth retarded and died between E10.0 and E10.5, owing to defects in extra-embryonic tissues. The placental labyrinth layer, where X-gal staining revealed expression of Fra1 , was reduced in size and largely...

...://www.biologists.com/Development/movies/dev4369.html * Author for correspondence (e-mail: [email protected] ) 27 05 2000 20 07 2000 © 2000 by Company of Biologists 2000 Connexin45 Cre- loxP system Cushion Embryonic lethality Gene targeting Heart The gap...