... compatible with targeted genetic manipulations. However, gene targeting, which is crucial for functional studies of embryonic stem cells, has not been exploited to date in NSC lines. Here, we deploy CRISPR/Cas9 technology to demonstrate a variety of sophisticated genetic modifications via gene targeting...

.... ( 2003 ). Optimized vector for conditional gene targeting in mouse embryonic stem cells . Biotechniques   34 , 1136 - 1138 , 1140 . Cui , C.-Y. , Smith , J. A. , Schlessinger , D. and Chan , C.-C. ( 2005 ). X-linked anhidrotic ectodermal dysplasia disruption yields a mouse...

...Nicholas Treen; Keita Yoshida; Tetsushi Sakuma; Haruka Sasaki; Narudo Kawai; Takashi Yamamoto; Yasunori Sasakura Custom designed nucleases can simplify gene targeting experiments and have the potential to allow these techniques to be performed in a wide range of organisms. Transcriptional activator...

...Luis Alberto Baena-Lopez; Cyrille Alexandre; Alice Mitchell; Laurynas Pasakarnis; Jean-Paul Vincent Gene targeting by ‘ends-out’ homologous recombination enables the deletion of genomic sequences and concurrent introduction of exogenous DNA with base-pair precision without sequence constraint...

.... , Deng H. ( 2010 ). Generation of homogeneous PDX1(+) pancreatic progenitors from human ES cell-derived endoderm cells . J. Mol. Cell Biol. 2 , 50 - 60 . Capecchi M. R. ( 2005 ). Gene targeting in mice: functional analysis of the mammalian genome for the twenty-first century . Nat...

... and subsequent X-inactivation in the mouse embryo. † Author for correspondence (e-mail: tsado@lab.nig.ac.jp ) * These authors contributed equally to this work 29 10 2008 © 2009. 2009 X-inactivation Xist Tsix Gene targeting Mouse embryo During the early development...

... . ( A ) Gene targeting strategy. The mouse Tshz3 locus consists of two exons and one intron,and spans more than 75 kb; the first and second exons encode, respectively, 13 and 1068 amino acids. The in-frame insertion of the lacZ -coding sequence (hatched box) and the neomycin expression cassette( pTK-neo...

... as a functional Xist RNA. ES cells harboring this allele( Tsix pA2lox ) were produced by gene targeting( Fig. 1B,C ). In parallel, we also used gene targeting to generate another mutant allele, Xist IVS2lox , which harbored the same intron without the mpA cassette at exactly the same position as the Tsix...

...). hg, hindgut; mn, metanephros; ur, ureter;Wd, Wolffian duct. Scale bars: 100 μm in A,B; 10 μm in C-J. We performed gene targeting of Dlgh1 . Functional Dlgh1 protein appeared to be absent from the generated mice. In the present study, we have described various abnormalities...

... regulatory role in Bmp signaling. However, previous gene targeting studies have demonstrated that the blockade of Bmp signals (e.g., the lack of Bmp7 ) can cause an even more drastic reduction in kidney development( Dudley et al., 1995 ; Luo et al., 1995 ). To test the possibility that some other related...

...) Gene targeting Epithelial cell polarity Epithelial cyst Heart development Epicardium Mouse © 2006. 2006 19 1 2006 * Author for correspondence (e-mail: ohnos@med.yokohama-cu.ac.jp ) EPP cell cyst formation is selectively affected in Par3 ΔE3/ΔE3 embryos...

... -1366. Passner, J. M., Ryoo, H. D., Shen, L., Mann, R. S. and Aggarwal,A. K. ( 1999 ). Structure of a DNA-bound Ultrabithorax-Extradenticle homeodomain complex. Nature 397 , 714 -719. Rong, Y. S. and Golic, K. G. ( 2000 ). Gene targeting by homologous recombination in Drosophila. Science...

... rather than neural defects. † Authors for correspondence (e-mail: march004@mc.duke.edu or dean.li@hmbg.utah.edu ) * These authors contributed equally to this work 2 12 2003 © 2004. 2004 Ccm1 Gene targeting Mice Angiogenesis Stroke Epilepsy Notch Efnb2...

... increases IGF bioavailability and mitogenic effectiveness in vitro through regulated cleavage of IGF-binding protein 4 (IGFBP4). To determine its function in vivo, we generated PAPPA-null mice by gene targeting. Mice homozygous for targeted disruption of the PAPPA gene were viable but 60% the size of wild...

... GnRH neurons Peripheral nerve Peripheral neuropathy Dysmyelination Homologous recombination Knockout Targeted inactivation Gene targeting COE2 O/E3 Olf/Ebf genes ( Garel et al.,1997 ; Hagman et al.,1993 ; Malgaretti et al.,1997 ; Wang and Reed,1993 ; Wang et al.,1997 ) (reviewed...

... in mature osteocytes during mineralization. *Author for correspondence (e-mail: peter.lonai@weizmann.ac.il ) 17 5 2002 © 2002. 2002 Transcriptional alternatives FGF Endochondral ossification Craniosynostosis Gene targeting Mouse Three of the four fibroblast growth...

... Gene targeting Mouse The parasympathetic nervous system represents one major division of the autonomic nervous system that, along with the sympathetic nervous system, plays a central role in controlling and coordinating the body’s homeostatic mechanisms. Peripheral parasympathetic innervation...

... development, the gene was inactivated by gene targeting. Foetuses lacking Fra1 were severely growth retarded and died between E10.0 and E10.5, owing to defects in extra-embryonic tissues. The placental labyrinth layer, where X-gal staining revealed expression of Fra1 , was reduced in size and largely...

.... Fertil. 107 , 255 – 264 . 10.1530/jrf.0.1070255 Papaioannou , V. and Johnson , R. ( 1993 ). Production of chimeras and genetically defined offspring from targeted ES cells . In Gene Targeting: A Practical Approach (ed. A. L. Joyner ), pp. 107 – 146 . New York: IRL...

..., Finland (e-mail: paivi.miettinen@helsinki.fi ) 23 May 2000 11 April 2000 © 2000 by Company of Biologists 2000 EGF receptor Gene targeting Pancreas Matrixmetalloproteinases Mouse The pancreas develops from foregut endoderm through the fusion of dorsal and ventral...