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Keywords: Gene gun
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Journal Articles
Journal:
Development
Development (2003) 130 (23): 5827–5838.
Published: 1 December 2003
... Helios Gene Gun (BioRad, Munich, Germany). The distance from the muzzle to the culture was 1 cm. The helium pressure was 160-200 psi(232×10 –4 Pa to 290×10 –4 Pa). To avoid excitotoxicity by Ca 2+ influx NMDA receptors were temporarily blocked by adding 3 mM kynorenic acid (Sigma-Aldrich, Deisenhofen...
