... ), Otx2 ( Millet et al., 1996 ), Ptf1a (ChEST1028o4), Lhx9 (Alessio Delogu, King’s College London, UK), Gdf7 ( Broom et al., 2012 ), Gbx2 ( Kiecker and Lumsden, 2004 ), Fgf8 and Sprouty2 ( Chambers et al., 2000 ); and mouse probes for: Atoh1 (Albert Basson, King’s College London, UK), Lhx9...

...N. Abimbola Sunmonu; Kairong Li; Qiuxia Guo; James Y. H. Li In vertebrates, the common expression border of two homeobox genes, Otx2 and Gbx2 , demarcates the prospective midbrain-hindbrain border (MHB) in the neural plate at the end of gastrulation. The presence of a compartment boundary...

...Bo Li; Sei Kuriyama; Mauricio Moreno; Roberto Mayor Wnt signalling is required for neural crest (NC) induction; however, the direct targets of the Wnt pathway during NC induction remain unknown. We show here that the homeobox gene Gbx2 is essential in this process and is directly activated by Wnt/β...

...Li Chen; Qiuxia Guo; James Y. H. Li Relatively little is known about the development of the thalamus,especially its differentiation into distinct nuclei. We demonstrate here that Gbx2 -expressing cells in mouse diencephalon contribute to the entire thalamic nuclear complex. However, the neuronal...

... in the mutant. We found a Dlx-regulated transcriptional enhancer in close proximity to Gbx2 , one of the Dlx5/6 -downstream genes, strongly suggesting that Gbx2 is a direct target of Dlx5/6 . We also showed that Pou3f3 is normally expressed in the maxillary (prospective upper jaw) but not mandibular arch...

...Samuel T. Waters; Mark Lewandoski Gbx2 is a homeobox gene that plays a crucial role in positioning the mid/hindbrain organizer (isthmus), which regulates midbrain and cerebellar development primarily through the secreted factor FGF8. In Gbx2 null homozygotes, rhombomeres (r) 1-3 fail to develop...

...Zhengshi Lin; Raquel Cantos; Maria Patente; Doris K. Wu Gbx2 is a homeobox-containing transcription factor that is related to unplugged in Drosophila. In mice, Gbx2 and Otx2 negatively regulate each other to establish the mid-hindbrain boundary in the neural tube. Here, we show that Gbx2...

...James Y. H. Li; Zhimin Lao; Alexandra L. Joyner The mouse homeobox gene Gbx2 is first expressed throughout the posterior region of the embryo during gastrulation, and becomes restricted to rhombomeres 1-3 (r1-3) by embryonic day 8.5 (E8.5). Previous studies have shown that r1-3 do not develop...

...). * Author for correspondence (e-mail: [email protected] ) 5 1 2005 ©2005. 2005 Midbrain-hindbrain boundary MHB/isthmic organizer Posteriorization Zebrafish Neural plate Morphogen wnt8 gbx1 otx2 gbx2 nodal fgf It has been postulated that, in vertebrate...

... Otx2 and Gbx2 . In order to provide novel insights into the roles of Otx2 and Gbx2 , we have studied mutant embryos carrying different dosages of Otx2 , Otx1 and Gbx2 . Embryos deficient for both OTX2 and GBX2 proteins ( hOtx1 2 /hOtx1 2 ; Gbx2 –/– ) show abnormal patterning of the anterior neural...

... differentiation in the midbrain and anterior hindbrain. Formation of this organizing center involves multiple steps, including positioning of the MHB within the neural plate, establishment of the organizer and maintenance of its regional identity and signaling activities. Juxtaposition of the Otx2 and Gbx2...

.../hindbrain genes that respond to FGF8, using a novel mouse brain explant culture system. We found that En2 and Gbx2 are the first genes to be induced by FGF8 in wild-type E9.5 diencephalic and midbrain explants treated with FGF8-soaked beads. By examining gene expression in En1/2 double mutant mouse embryos...

..., but also can induce the hindbrain gene Gbx2 and alter the expression of Wnt1 in both midbrain and caudal forebrain explants. We also show that FGF8b- soaked beads can repress Otx2 in midbrain explants. Furthermore, Wnt1-Fgf8b transgenic embryos in which the same Wnt1 regulatory element is used to express...

... tissues and involves a diffusible signal. * Author for correspondence (e-mail: [email protected] ) 19 06 1999 23 08 1999 © 1999 by Company of Biologists 1999 Isthmus Boundary Hindbrain Midbrain Rhombomere Fgf8 Gbx2 Cell signalling Regeneration The isthmus...

...Karen Montzka Wassarman; Mark Lewandoski; Kenneth Campbell; Alexandra L. Joyner; John L. R. Rubenstein; Salvador Martinez; Gail R. Martin ABSTRACT Analysis of mouse embryos homozygous for a loss-of-function allele of Gbx2 demonstrates that this homeobox gene is required for normal development...