... of 2D gastruloids comprising wild-type, TBXT heterozygous or TBXT null human induced pluripotent stem cells reveal that varying TBXT dosage does not compromise the ability of a cell to differentiate into nascent mesoderm, but instead directly influences the temporal progression of the epithelial...

... to signalling cocktails and aggregated into large embryonic bodies, but these do not recapitulate the native embryonic environment during PGC formation. Here, we show that mouse gastruloids, a three-dimensional in vitro model of gastrulation, contain a population of gastruloid-derived PGCLCs (Gld-PGCLCs...

...Evan J. Underhill; Jared E. Toettcher ABSTRACT Many developmental processes rely on the localized activation of receptor tyrosine kinases and their canonical downstream effectors Erk and Akt, yet the specific roles played by each of these signals is still poorly understood. Gastruloids, 3D cell...

... Gastruloid Organogenesis Stem cell Michigan-Cambridge Research Initiative National Institutes of Health http://dx.doi.org/10.13039/100000002 R21 NS113518 R21 HD100931 R21 HD105126 R21 HD105192 R21 HD109635 R01 GM143297 National Science Foundation http://dx.doi.org...

... been studied in the context of toxicology, whereas gastruloids have shown a good predictivity for known in vivo teratogenicity of a small selection of compounds ( Mantziou et al., 2021 ), and multiple organoid models have already been demonstrated to predict adverse chemical reactions (reviewed...

... and extra-embryonic stem cells as well as the functional impact on differentiation potential. We find a temporal and cell type-specific transcriptional response including an early primitive streak signature in hypoxic embryonic stem cells mediated by HIF1α. Using a 3D gastruloid differentiation model, we...

...Iain Martyn; Eric D. Siggia; Ali H. Brivanlou ABSTRACT Although fate maps of early embryos exist for nearly all model organisms, a fate map of the gastrulating human embryo remains elusive. Here, we use human gastruloids to piece together a rudimentary fate map for the human primitive streak (PS...