... junction communication. We report that nuclei display spontaneous calcium transients, and that changes in the activity patterns coincide with the course of nucleogenesis. We also find that these activity patterns are disrupted by manipulating cadherin or gap junction expression. Furthermore, inhibition...

..., Hedgehog, BMP and EGF pathways are used to mediate soma-germline communication. Here, we demonstrate that gap junctions may also mediate direct, bi-directional signalling between the soma and germ line. When gap junctions between the soma and germ line are disrupted, germline differentiation is blocked...

... signaling event. Intercellular communication between AWCs and non-AWC neurons via a NSY-5 gap junction network coordinates AWC asymmetry. However, the nature of intercellular signaling across the network and how individual non-AWC cells in the network influence AWC asymmetry is not known. Here, we...

... signal from the somatic cells is cGMP. Using FRET-based cyclic nucleotide sensors in follicle-enclosed mouse oocytes, we find that cGMP passes through gap junctions into the oocyte,where it inhibits the hydrolysis of cAMP by the phosphodiesterase PDE3A. This inhibition maintains a high concentration...

...Greg FitzHarris; Jay M. Baltz Oocytes grow within ovarian follicles in which the oocyte is coupled to the surrounding granulosa cells by gap junctions. It was previously found that small growing oocytes isolated from juvenile mice and freed of their surrounding granulosa cells (denuded) lacked...

... left or right coronary artery, and accessory coronary arteries. However, we show that proepicardial cells also express connexin43 gap junctions abundantly. The proepicardial cells are functionally well coupled, and this coupling is significantly reduced with the loss of connexin43 function. Further...

...Olaf Krüger; Achim Plum; Jung-Sun Kim; Elke Winterhager; Stephan Maxeiner; Gaby Hallas; Susanne Kirchhoff; Otto Traub; Wouter H. Lamers; Klaus Willecke ABSTRACT In order to reveal the biological function(s) of the gap-junction protein connexin 45 (Cx45), we generated Cx45 -deficient mice...

...Boris Risek; F. George Klier; Norton B. Gilula ABSTRACT The expression of four different gap junction gene products ( α 1 , β 1 , β 2 , andβ 3 ) has been analysed during rat skin development and the hair growth cycle. Both α 1 (C×43) and β 2 (C×26) connexins were coexpressed in the undifferentiated...

...Kirsi Sainio; Scott F. Gilbert; Eero Lehtonen; Miyuki Nishi; Nalin M. Kumar; Norton B. Gilula; Lauri Saxén ABSTRACT The expression of three gap junction (GJ) proteins, α 1 (Cx43), β 1 (Cx32), and β 2 (Cx26), and their transcripts were examined during the ontogeny of the mouse and rat kidney...

... be related to previous ultrastructural studies by others on the distribution of gap junctions in the wool follicle. These results show that junctional communication, with its consequent intercellular spread of small ions and molecules, is associated with uniformity of expression and behaviour within cell...

..., since we did not observe any significant number of cytoplasmic bridges at the EM and G j is sensitive to octanol, it is probable that blastomeres in the 2-cell and 32-cell embryos are in communication by gap junctions. In order to compare G j in the two stages and to circumvent problems of cell size...

...Boris Risek; Norton B. Gilula ABSTRACT The expression of three different members of the gap junction multigene family, a x (Cx43), ft (Cx32), and p2 (Cx26), was analysed in the rat implantation chamber (a structural unit containing fetal, extraembryonic and maternal components within the pregnant...

...Francesca Allen; Cheryll Tickle; Anne Warner The role of gap junctional communication during patterning of the chick limb has been investigated. Affinity-purified antibodies raised against rat liver gap junctional proteins were used to block communication between limb mesenchyme cells. Co-injection...

... of Biologists 1989 gap junctions junctional communication repeated epilation proliferative control skin dye injection Gap junctions provide pathways for direct cell-cell communication in most animal tissues ( Finbow, 1982 ). Coupled cell populations are partial syncytia (termed...

...Harry Goodall ABSTRACT Three treatments that prevent cell flattening during compaction of the mouse preimplantation embryo were assessed for their effects on the onset of gap junctional communication. Medium low in calcium (LCM) and an antiserum to an embryonal carcinoma cell line (anti-EC; Johnson...

...Anne E. Warner ABSTRACT The possibility that communication through gap junctions may be important during embryonic development has often been raised since gap junctions were first described between early embryonic cells. It is now known that this direct cell-to-cell communication pathway disappears...