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Keywords: GFP
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Journal Articles
Journal: Development
Development (2018) 145 (2): dev148874.
Published: 26 January 2018
... and manipulate proteins, and highlights the recent application of such tools in developmental and synthetic biology. Protein binder Nanobody DARPin Monobody Protein degradation GFP Forward and reverse genetic approaches have been key to expanding our knowledge of gene function during...
Journal Articles
Journal: Development
Development (2014) 141 (20): 4006–4017.
Published: 15 October 2014
... permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. GFP Epithelium Morphogenesis Protein trap Fluorescent proteins have revolutionised our ability to observe proteins in live tissues. Single gene studies now...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2012) 139 (22): 4261–4270.
Published: 15 November 2012
... and genetic lineages of the mesencephalon and rhombomere 1 . Neuron 43 , 345 - 357 . Funding This work was supported by an MRC studentship to E.R.B., by a grant from the Wellcome Trust, and by grants from the BBSRC to R.J.T.W. and to Helen Sang (Roslin Institute/GFP-tg eggs). Deposited in PMC...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2012) 139 (9): 1691–1699.
Published: 1 May 2012
... of AB or AB/TU wild-type or Tg(hsp70:Gal4-VP16), Tg(UAS:GFP)kca33/+, Tg[(mnx1:Gal4-VP16)b1222,UAS:GFP], Et[(–1.5hsp70l:Gal4-VP16)s1102t,(UAS-E1b:Kaede)s1999t] and Tg(3.1neurog1:GFP)sb2 transgenic lines (see http://zfin.org/ ). Fish were staged by hours post-fertilization (hpf) at 28.5°C ( Kimmel et al...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2010) 137 (3): 373–387.
Published: 1 February 2010
... of biological processes was recognized through the award of the Nobel Prize in Chemistry to Osamu Shimomura, Martin Chalfie and Roger Tsien in 2008, for the discovery and development of the most famous FP, green fluorescent protein (GFP). The recent development of fluorescent tools to study the dynamics...
Journal Articles
Journal Articles
Journal Articles
Journal: Development
Development (2004) 131 (22): 5703–5716.
Published: 15 November 2004
... Cartilage Neural crest Segmentation Fgf8 Fgf3 acerebellar GFP Zebrafish The cartilages and bones that form the skeleton of the face and, in mammals, the middle ear, are derived from a specialized population of ectomesenchyme, the cranial neural crest( Le Douarin, 1982 ; Weston et al., 2004...
Includes: Multimedia, Supplementary data
Journal Articles
Journal: Development
Development (2004) 131 (15): 3717–3726.
Published: 1 August 2004
..., Parkville, Victoria 3010, Australia 7 4 2004 © 2004. 2004 MET c-MET Neurons Process growth GFP Slice cultures Hepatocyte growth factor (HGF), or scatter factor, is a secreted protein that exerts a variety of effects on many cell types by binding to the MET receptor tyrosine...
Journal Articles
Journal: Development
Development (2003) 130 (24): 5885–5894.
Published: 15 December 2003
...%, respectively, but there is no difference in its interaction with TRY. Furthermore, TRY has the ability to prevent the GL1 GL3 interaction and the GL1 gl3-sst interaction is even more sensitive to TRY. Analysis of plants expressing functional GFP-tagged versions of GL1, GL3 and TRY show that the proteins...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2003) 130 (20): 4955–4962.
Published: 15 October 2003
... Drosophila Hemocytes Clonal analysis Cell lineage Transplantation Blood GFP Lymph gland Macrophage Hematopoiesis The hemolymph of Drosophila contains hemocytes that either circulate freely through the body cavities or are sessile, being associated with various tissues and organs...
Journal Articles
Journal: Development
Development (2003) 130 (18): 4351–4362.
Published: 15 September 2003
... KNOTTED1 ( KN1 ) gene acts non cell-autonomously in the maize leaf, and KN1 was the first plant protein shown to traffic cell-to-cell, presumably through plasmodesmata. We have compared the intercellular trafficking of green fluorescent protein (GFP)fusions of KN1 and Arabidopsis KN1-related homeobox...
Journal Articles
Journal: Development
Development (2002) 129 (20): 4719–4728.
Published: 15 October 2002
...-mail: richard.wingate@kcl.ac.uk ) 12 6 2002 Chick netrin slit Robo GFP Time-lapse confocal microscopy Examination of GFP-chimaeras at E6 revealed rhombic lip derivatives that have migrated into ventral rhombomere 1 ( Fig. 1 C). The leading processes of cells turn either rostrally or caudally...
Includes: Multimedia, Supplementary data
Journal Articles
Journal: Development
Development (2001) 128 (13): 2517–2524.
Published: 1 July 2001
...Robert Klapper; Sandra Heuser; Thomas Strasser; Wilfried Janning In order to reveal syncytia within the visceral musculature of Drosophila melanogaster , we have combined the GAL4/UAS system with the single-cell transplantation technique. After transplantation of single cells from UAS-GFP donor...
Journal Articles
Journal: Development
Development (2000) 127 (24): 5253–5263.
Published: 15 December 2000
... for correspondence (e-mail: mgoetz@neuro.mpg.de ) 03 10 2000 14 11 2000 © 2000 by Company of Biologists 2000 Cerebral cortex Radial glial cells Neuronal precursors Glial precursors Glutamate astrocyte-specific transporter (GLAST) GFP Human GFAP-promoter Clonal analysis...
Journal Articles
Journal: Development
Development (2000) 127 (19): 4253–4264.
Published: 1 October 2000
... how the microtubule network polarizes using a GFP-tubulin construct that allows germ-cell microtubules to be visualized with greater sensitivity than in previous studies. Unexpectedly, microtubules are seen to associate with the fusome, an asymmetric germline-specific organelle, which elaborates...
Journal Articles
Journal: Development
Development (2000) 127 (16): 3467–3474.
Published: 15 August 2000
...-cell stage embryos and single blastomeres on the opposite side of the embryo in 29 8-cell embryos with synthetic mRNA for GFP to trace their fate in vitro until the blastocyst stage. In 44 injected embryos (25 with blastomeres labelled near and 19 with blastomeres labelled opposite the polar body) we...
Journal Articles
Journal Articles
Journal Articles
Journal: Development
Development (1998) 125 (19): 3895–3903.
Published: 1 October 1998
... regulatory light chain null mutant (mlcR − ) using cell-type-specific promoters. While mlcR − cells fail to progress beyond the mound stage, expression of RLC from the prestalk promoter, ecmA, produces culminants with normal stalks but with defects in spore cell localization. When GFP-marked prestalk...