.... Here, we investigated whether the key cardiac transcription factor GATA4 is required for neonatal mouse heart regeneration. Using the neonatal mouse heart cryoinjury and apical resection models with an inducible loss of GATA4 specifically in cardiomyocytes, we found severely depressed ventricular...

...Shouhong Xuan; Lori Sussel GATA4 and GATA6 are zinc finger transcription factors that have important functions in several mesodermal and endodermal organs, including heart, liver and pancreas. In humans, heterozygous mutations of either factor are associated with pancreatic agenesis; however...

... Primitive endoderm Epiblast Mouse blastocyst ICM aPKC GATA4 Pluripotency The development of the mammalian foetus is completely dependent on support from the extra-embryonic tissues, which provide the connection with the mother and are important signalling centres for patterning...

... lineage traced gata4 + and nkx2.5 + ALPM populations predicted to contain SHF progenitors, based on evolutionary conservation of ALPM patterning. Traced cells were identified in SHF-derived distal ventricular myocardium and in three lineages in the outflow tract (OFT). We confirmed the extent...

... jumonji ( Jmj, Jarid2 ), which encodes the repressor of cyclin D1. Analysis of Jmj/ cyclin D1 double mutant mice showed that Jmj was required for normal differentiation and normal expression of GATA4 protein through cyclin D1. Analysis of transgenic mice revealed that enhanced expression of cyclin D1...

... encodes a gap junction protein required for normal atrioventricular (AV) delay in mice, is necessary and sufficient to direct expression to the developing AV conduction system (AVCS). Moreover, we show that this enhancer requires Tbx5 and Gata4 for proper expression in the conduction system, and Gata4...

... stage, during asymmetric signal propagation, excessive Wnt/β-catenin signaling inhibits the transmission of asymmetric cues from the lateral plate mesoderm (LPM) to the cardiac field but not to the developing gut; as such, it only regulates heart laterality. Molecular analysis identifies Gata4...

...Nikolay L. Manuylov; Fatima O. Smagulova; Lyndsay Leach; Sergei G. Tevosian We have demonstrated previously that mammalian sexual differentiation requires both the GATA4 and FOG2 transcriptional regulators to assemble the functioning testis. Here we have determined that the sexual development...

... of portions of the atrial and ventricular septae, and the generation of thin, pliable valves. Gata4 , which encodes a zinc finger transcription factor, is expressed in the endothelium and mesenchyme of the AV valves. Using a Tie2-Cre transgene, we selectively inactivated Gata4 within endothelial-derived cells...

...Anabel Rojas; Sarah De Val; Analeah B. Heidt; Shan-Mei Xu; James Bristow; Brian L. Black The GATA family of zinc-finger transcription factors plays key roles in the specification and differentiation of multiple cell types during development. GATA4 is an early regulator of gene expression during...

... that myocardin is sufficient to activate transcription of a wide range of cardiac and smooth muscle differentiation markers in non-muscle cell types. We also demonstrate that, for the myosin light chain 2 gene ( MLC2 ), myocardin cooperates with the zinc-finger transcription factor Gata4 to activate expression...

...-cultivated with β-galactosidase-negative GATA4-transformed endoderm-like cells of AB2.2 origin. After 5 days of culture, the EBs were fixed, stained for β-galactosidase, embedded in plastic and counterstained with neutral red ( Fig. 4A-D ). Wild-type AB2.2 cells developed into cystic EBs with external...

.... This conserved enhancer contains two consensus GATA binding sites that are efficiently bound by the zinc finger transcription factor GATA4 and are completely required for enhancer function in vivo. This enhancer also contains two perfect consensus sites for the LIM-homeodomain protein ISL1. We show...

... tissue. We show that the transcription factor GATA4,which has been implicated in regulating cardiac gene expression, is sufficient to induce cardiac differentiation in Xenopus embryonic ectoderm(animal pole) explants, frequently resulting in beating tissue. Lineage labelling experiments demonstrate...

.... In the absence of Sry , or if Sry is expressed at insufficient levels, the support cell precursors differentiate as granulosa cells, thus initiating the ovarian pathway. The molecular mechanisms upstream and downstream of Sry are not well understood. We demonstrate that the transcription factor GATA4 and its co...

...Frank Reifers; Emily C. Walsh; Sophie Léger; Didier Y. R. Stainier; Michael Brand ABSTRACT Vertebrate heart development is initiated from bilateral lateral plate mesoderm that expresses the Nkx2 . 5 and GATA4 transcription factors, but the extracellular signals specifying heart precursor gene...