... and glutamatergic neurons that have been associated with a variety of functions, including sleep regulation. However, the molecular characteristics and development of MRF neurons are poorly understood. As the transcription factor, Gata2 is required for the development of all GABAergic neurons derived from...

... ABSTRACT Mutations in the transcription factor GATA2 cause lymphedema. GATA2 is necessary for the development of lymphatic valves and lymphovenous valves, and for the patterning of lymphatic vessels. Here, we report that GATA2 is not necessary for valvular endothelial cell (VEC) differentiation. Instead...

..., we show that genetic redundancy of Gata3 with paralog Gata2 in trophoblast progenitors ensures the successful progression of both pre- and postimplantation mammalian development. Stage-specific gene deletion in trophoblasts reveals that loss of both GATA genes, but not either alone, leads...

... expressing vesicular glutamate transporter 3 ( Vglut3 ) or serotonin transporter ( Sert ). These populations differ in their requirements for transcription factors Gata2 and Gata3, which are activated in the post-mitotic precursors. Gata2 operates upstream of Gata3 as a cell fate selector in both populations...

...Yangsook Song Green; Sunjong Kwon; Mizuho S. Mimoto; Yuanyuan Xie; Jan L. Christian In Xenopus laevis , bone morphogenetic proteins (Bmps) induce expression of the transcription factor Gata2 during gastrulation, and Gata2 is required in both ectodermal and mesodermal cells to enable mesoderm...

... of these regulatory neurons are poorly understood. We show here that different GABAergic and glutamatergic subgroups associated with the monoaminergic nuclei express specific transcription factors. These neurons share common origins in the ventrolateral rhombomere 1, where the postmitotic selector genes Tal1 , Gata2...

... in mice have suggested that GATA2 might function at early stages within hemogenic endothelium. Two orthologs of Gata2 exist in zebrafish: gata2a and gata2b . Here, we report that gata2b expression initiates during the convergence of PLM, becoming restricted to emerging HSCs. We observe Notch-dependent...

...Sini-Maaria Virolainen; Kaia Achim; Paula Peltopuro; Marjo Salminen; Juha Partanen Diverse mechanisms regulate development of GABAergic neurons in different regions of the central nervous system. We have addressed the roles of a proneural gene, Ascl1 , and a postmitotic selector gene, Gata2...

... the developmental origin of the VTA and SNpr GABAergic neurons by genetic fate mapping. We demonstrate that the majority of these GABAergic neurons originate outside the midbrain, from rhombomere 1, and move into the ventral midbrain only as postmitotic neuronal precursors. We further show that Gata2, Gata3...

... distribution of competence is established during gastrulation paralleling the dorsal restriction of neural competence. We further show that Dlx3 and GATA2 are required cell-autonomously for panplacodal and epidermal marker expression in the non-neural ectoderm, while ectopic expression of Dlx3 or GATA2...

..., CA 94547, USA Competing interests statement The authors declare no competing financial interests. 3 8 2011 © 2011. gata2 Vascular development Zebrafish Zinc-finger nuclease The attributes of the zebrafish have established it as a powerful model...

... ascribed the property of regulating the expression of numerous endothelium-specific genes. However,the transcriptional regulatory hierarchy governing Gata2 activation in endothelial cells has not been fully explored. Here, we document GATA-2 endothelial expression during embryogenesis by following GFP...

... mechanisms, we compared gene expression profiles between embryonic stem (ES) cell-derived hemangioblasts (Blast-Colony-Forming Cells, BL-CFCs)and their differentiated progeny, Blast cells. Bioinformatic analysis indicated that BL-CFCs resembled other stem cell populations. A role for Gata2 , one of the BL...

...Marsha E. Lucas; Frank Müller; Roland Rüdiger; Paul D. Henion; Hermann Rohrer The basic helix-loop-helix transcription factor Hand2 , together with Ascl1, Phox2a, Phox2b and Gata2/Gata3 , is induced by bone morphogenetic proteins in neural crest-derived precursor cells during sympathetic neuron...

... and morphogenesis. This probably relies on complex interplay between mechanisms that control cell cycle, specification and differentiation. In this respect, we have studied the possible implication of GATA2, a transcription factor that is involved in several neuronal specification pathways, in the control...

..., which includes Mash1, Phox2b, Hand2 and Phox2a. Here, we have studied the function of Gata2 and Gata3 zinc-finger transcription factors in autonomic neuron development. In the chick, Gata2 but not Gata3 is expressed in developing sympathetic precursor cells. Gata2 expression starts after Mash1, Phox2b...

... © 2003. 2003 Forebrain Zebrafish Commissural axon Neuronal scaffold Growth cone Intermediate target Growth cone morphology Growth cone behavior GATA2 Axon pathway During development of the central nervous system (CNS), neurons differentiate and connect with one other, enabling...

... factors, Pit1, Gata2, Egr1 and Sf1 , on a threshold level of PITX2. The related Pitx1 gene has a role in hormone gene transcription, and it is important late in ontogeny for the final expansion of the differentiated cell types. Pitx1 and Pitx2 have overlapping functions in the expansion of Rathke’s pouch...

...Yinghui Zhou; Masayuki Yamamoto; James Douglas Engel ABSTRACT During embryogenesis, transcription factor GATA2 is expressed in a variety of distinct cell types, and earlier experiments showed that GATA2 is a vital regulator of both hematopoiesis and urogenital development. Despite the fact...

... on functional GATA2, which in turn is under the control of Hoxb1 . In particular, the absence of Hoxb1 results in the loss of GATA2 expression in r4 and the absence of GATA2 results in the loss of GATA3 expression. The lack of GATA3 expression in r4 inhibits the projection of contralateral vestibuloacoustic...