... found that one factor in the middle of the cascade, END-1, which straddles the distinct events of specification and differentiation, functions in both processes. Finally, we reveal roles for key GATA factors in establishing spatial regulatory state domains by repressing other fates, thereby defining...

...Brett R. Lancaster; James D. McGhee ABSTRACT We define a quantitative relationship between the affinity with which the intestine-specific GATA factor ELT-2 binds to cis -acting regulatory motifs and the resulting transcription of asp-1 , a target gene representative of genes involved...

... responds to overexpression of the GATA transcription factors END-1 and END-3, which specify the intestine, as well as to overexpression of the two GATA factors that are normally involved in intestinal differentiation, ELT-7 and ELT-2 itself. Little is known about the molecular mechanisms underlying...

... functions of FGF/MAPK signaling in this decision. First, the pathway needs to be inhibited to make the PrE-like gene expression program accessible for activation by GATA transcription factors in ESCs. In a second step, MAPK signaling levels determine the threshold concentration of GATA factors required...

[email protected] ) 17 3 2003 © 2003. 2003 Drosophila Heart development GATA factor ETS-domain proteins Comparative studies between vertebrates and Drosophila highlight a significant conservation in the embryology and molecular regulation of heart development (reviewed by Bodmer...

.... LIN-39/CEH-20 heterodimers bind two consensus HOX/PBC sites in a vulval enhancer region of egl-18/elt-6 , one of which is essential for vulval expression of egl-18/elt-6 reporter constructs. These findings demonstrate that the EGL-18 and ELT-6 GATA factors are essential, genetically redundant...

... epidermal cell types are specified. We report that elt-5 and -6 , adjacent genes encoding GATA factors, are essential for the development of the lateral epidermal cells, the seam cells. Inhibition of elt-5 and - 6 function by RNA-mediated interference results in penetrant late embryonic and early larval...

..., in the Xenopus animal cap causes ectopic endodermal gene expression and suggests a conserved role for GATA factors in early endoderm formation ( Shoichet et al., 2000 ). Additionally, when injected into Xenopus animal caps, GATA4 and GATA5 induce endodermal gene expression, and GATA5 can respecify...

... specification. In Drosophila , the existence of a cardiogenic GATA factor has been implicated through the analysis of a cardial cell enhancer of the muscle differentiation gene D-mef2 . We show that the GATA gene pannier is expressed in the dorsal mesoderm and required for cardial cell formation while...

... understood. We use in vivo footprinting of mouse embryonic endoderm cells to show that a DNA-binding site for GATA factors is occupied on a liver-specific, transcriptional enhancer of the serum albumin gene. GATA site occupancy occurs in gut endoderm cells at their pluripotent stage: the cells have...

... in regulating the synthesis of related placental hormones. * Author for correspondence (e-mail: [email protected] ) 20 11 1996 © 1997 by Company of Biologists 1997 angiogenesis GATA factor placenta placental lactogen proliferin transcription factor trophoblast...

..., -5 and -6 are expressed in various organs of endo-dermal origin, such as intestine and liver, and are impli-cated in endodermal differentiation. Here we report that the Drosophila gene serpent ( srp ) encodes the previously described GATA factor ABF. The multiple functions of srp in Drosophila...

... expresses endogenous GATA-2 and GATA-3. Furthermore, overexpression of GATA factors in Rcho-1 cells stimulates transcription from a co-transfected placental lactogen I gene promoter. Most significantly, expression of GATA-2 or GATA-3 was found to induce transcription from this promoter in transfected non...