.... In this study, we adapted RNAscope fluorescent in situ hybridization technology for use on whole-mount mouse primary myofibers, a preparation that isolates single myofibers with their associated muscle stem cells remaining in their niche. This method can be combined with immunofluorescence, enabling...

...Lars Martin Jakt; Satoko Moriwaki; Shinichi Nishikawa Oligonucleotide-based fluorescent in situ hybridization (FISH) coupled with high-resolution high-sensitivity microscopy allows the visualization of single RNA molecules within fixed cells and tissues as distinct foci. We show here...

... Xenopus achaete-scute homolog, provides an early marker of planar neural induction and position along the mediolateral axis of the neural plate . Development 119 , 221 – 232 . 10.1242/dev.119.1.221 Tyramide Fluorescent in situ hybridization Whole-mount confocal microscopy xk81...