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Keywords: Fluorescence
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Journal Articles
Journal: Development
Development (2021) 148 (18): dev199611.
Published: 8 September 2021
.... Hence, there is a pressing demand for a coding-free, intuitive and scalable solution that analyses such image data in an automated yet rapid manner. Here, we present MOrgAna, a Python-based software that implements machine learning to segment images, quantify and visualize morphological and fluorescence...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2021) 148 (18): dev199744.
Published: 7 September 2021
... single-molecule studies, as well as to inspire the application of SMT to their own work. We start by overviewing the basics of and motivation for SMT experiments, and the trade-offs involved when optimizing parameters. We then cover key technologies, including fluorescent labelling, excitation...
Journal Articles
Journal: Development
Development (2018) 145 (4): dev156257.
Published: 14 February 2018
... derivate matures predominantly into a red fluorescent isoform. By contrast, a green fluorescent isoform is favored by low oxygen levels. Ratiometric analysis of green and red fluorescence after a pulse of Timer expression in Drosophila larvae provides a record of the history of tissue oxygenation during...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2015) 142 (5): 1016–1020.
Published: 1 March 2015
...Andrea Bassi; Benjamin Schmid; Jan Huisken Fluorescently labeled structures can be spectrally isolated and imaged at high resolution in living embryos by light sheet microscopy. Multimodal imaging techniques are now needed to put these distinct structures back into the context of the surrounding...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (1989) 106 (1): 95–103.
Published: 1 May 1989
... of ftz mutant embryos. We describe here novel intermediate patterns of ftz protein expression which were detected in younger embryos by using anti- ftz antibodies and a sensitive fluorescence/immunoperoxidase technique (‘filtered fluorescence imaging’, FFI). Striped patterns of ftz protein evolved...
Journal Articles
Journal: Development
Development (1988) 103 (4): 675–686.
Published: 1 August 1988
...Douglas R. Kellogg; Tim J. Mitchison; Bruce M. Alberts We describe the preparation of novel fluorescent derivatives of rabbit muscle actin and bovine tubulin, and the use of these derivatives to study the behaviour of actin filaments and microtubules in living Drosophila embryos, in which...