... by phosphorylation of receptors or components of signaling pathways. We identified Cables1 as a linker between floor-plate exit of commissural axons, regulated by Slit/Robo signaling, and the rostral turn of post-crossing axons, regulated by Wnt/Frizzled signaling. Cables1 localizes β-catenin, phosphorylated...

...Chloé Dominici; Quentin Rappeneau; Pavol Zelina; Stéphane Fouquet; Alain Chédotal ABSTRACT During development, precerebellar neurons migrate tangentially from the dorsal hindbrain to the floor plate. Their axons cross it but their cell bodies stop their ventral migration upon reaching the midline...

...Navid Nouri; Rajeshwar Awatramani The mesodiencephalic floor plate (mdFP) is the source of diverse neuron types. Yet, how this structure is compartmentalized has not been clearly elucidated. Here, we identify a novel boundary subdividing the mdFP into two microdomains, defined by engrailed 1 ( En1...

... germ layer plasticity, these cells never give rise to midline tissues of the notochord, floor plate and dorsal endoderm, raising the question of whether midline tissues also arise from basal posterior progenitors after gastrulation. We show in zebrafish that local posterior signals specify germ layer...

... and localisation of key Shh pathway components, thus sensitising cells to Shh. Notch Shh Embryo Chick Mouse Cilia Notochord Floor plate P3 progenitors The notochord is the source of a signal, Sonic Hedgehog (Shh), that patterns the dorsoventral aspect of the neural tube ( Echelard et al...

... interests. 30 7 2013 6 1 2014 © 2014. Published by The Company of Biologists Ltd 2014 Shh Sulfatase1 Floor plate Neural cell fate Spinal cord Zebrafish In the developing ventral spinal cord, Sonic hedgehog (Shh) serves crucial roles in regulating expression...

...Abed AlFatah Mansour; Sophie Khazanov-Zisman; Yaara Netser; Avihu Klar; Nissim Ben-Arie During embryogenesis, the dorsal roof plate and the ventral floor plate (FP) act as organizing centers to pattern the developing neural tube. Organizer-secreted morphogens provide signals that are interpreted...

...Emmanouil Metzakopian; Wei Lin; Mali Salmon-Divon; Heidi Dvinge; Elisabet Andersson; Johan Ericson; Thomas Perlmann; Jeffrey A. Whitsett; Paul Bertone; Siew-Lan Ang The transcription factors Foxa1 and Foxa2 promote the specification of midbrain dopaminergic (mDA) neurons and the floor plate...

.... Dotted domains indicate that only a very small number of cells remain. Medial floor plate is not shown but is present (medial to row 1) in both WT and MZsmo mutants. The dorsal limit of the V3 domain is hard to determine precisely because both tal2 and gata3 are also expressed by more dorsal cells...

... study shows that a subset of anterior floor plate-like cells gives rise to Fgf3 + SOX3 + progenitor cells, demonstrates a dual origin of infundibular cells and reveals a crucial role for FGF signalling in governing extended infundibular growth. To analyse this behaviour in detail, we examined serial...

... patterning, and describe a feedback circuit between the Shh pathway and homeodomain transcription factors that establishes non-graded regulation of Shh signaling activity. Nkx2 proteins intrinsically strengthen Shh responses in a feed-forward amplification and are required for ventral floor plate and p3...

...Keiji Inohaya; Yoshiro Takano; Akira Kudo The floor plate is a key organizer that controls the specification of neurons in the central nervous system. Here, we show a new role of the floor plate: segmental pattern formation of the vertebral column. Analysis of a spontaneous medaka mutant, fused...

...Shona D. Gray; J. Kim Dale Hensen's node of the chick embryo contains multipotent self-renewing progenitor cells that can contribute to either the floor plate or the notochord. Floor plate cells are a population of epithelial cells that lie at the ventral midline of the developing neural tube...

... trajectories parallel to the floor plate. To identify longitudinal guidance mechanisms in vivo, the overall role of floor plate tissue and the specific roles of Slit/Robo signals were tested. Ectopic induction or genetic deletion of the floor plate diverted longitudinal axons into abnormal trajectories...

[email protected] ) * Present address: Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto 860-0811, Japan 12 4 2006 © 2006. 2006 bHLH Floor plate Isthmus Rhombomere boundary Roof plate Zona limitans intrathalamica Mouse The developing...

... Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 2-3-10 Kandasurugadai,Chiyoda-ku, Tokyo 101-0062, Japan 4 4 2006 © 2006. 2006 Xenopus Midline Cholesterol Floor plate Smith-Lemli-Opitz syndrome (SLOS) Sonic hedgehog The members...

...Silvia L. López; María V. Rosato-Siri; Paula G. Franco; Alejandra R. Paganelli; Andrés E. Carrasco We have previously shown that the early Xenopus organiser contains cells equally potent to give rise to notochord or floor plate, and that Notch signalling triggers a binary decision, favouring...

...Iain Patten; Paul Kulesa; Michael M. Shen; Scott Fraser; Marysia Placzek To begin to reconcile models of floor plate formation in the vertebrate neural tube, we have performed experiments aimed at understanding the development of the early floor plate in the chick embryo. Using real-time analyses...

...Yongsu Jeong; Douglas J. Epstein The establishment of the floor plate at the ventral midline of the CNS is dependent on an inductive signaling process mediated by the secreted protein Sonic hedgehog (Shh). To understand molecularly how floor plate induction proceeds we identified a Shh-responsive...

...Jing Tian; Caleb Yam; Gayathri Balasundaram; Hui Wang; Aniket Gore; Karuna Sampath The floor plate, a specialized group of cells in the ventral midline of the neural tube of vertebrates, plays crucial roles in patterning the central nervous system. Recent work from zebrafish, chick, chick-quail...