... by phosphorylation of receptors or components of signaling pathways. We identified Cables1 as a linker between floor-plate exit of commissural axons, regulated by Slit/Robo signaling, and the rostral turn of post-crossing axons, regulated by Wnt/Frizzled signaling. Cables1 localizes β-catenin, phosphorylated...

...Chloé Dominici; Quentin Rappeneau; Pavol Zelina; Stéphane Fouquet; Alain Chédotal ABSTRACT During development, precerebellar neurons migrate tangentially from the dorsal hindbrain to the floor plate. Their axons cross it but their cell bodies stop their ventral migration upon reaching the midline...

...Navid Nouri; Rajeshwar Awatramani The mesodiencephalic floor plate (mdFP) is the source of diverse neuron types. Yet, how this structure is compartmentalized has not been clearly elucidated. Here, we identify a novel boundary subdividing the mdFP into two microdomains, defined by engrailed 1 ( En1...

... germ layer plasticity, these cells never give rise to midline tissues of the notochord, floor plate and dorsal endoderm, raising the question of whether midline tissues also arise from basal posterior progenitors after gastrulation. We show in zebrafish that local posterior signals specify germ layer...

... in NIH-3T3 cells is that cilia were significantly longer at the P ≤0.001 level [ F (2,1534)=128.557, P ≤2.2×10 −16 ; Fig. 6 C,D compared with 6A,B; Fig. 7 A]. In vivo , previous reports have shown the floor plate cilia become significantly longer than P3 cilia ( Cruz et al., 2010 ; Fig. 7...

... ). Dual origin of the floor plate in the avian embryo . Development 129 , 4785 – 4796 . Chung A. Y. , Kim S. , Kim E. , Kim D. , Jeong I. , Cha Y. R. , Bae Y. K. , Park S. W. , Lee J. , Park H. C. ( 2013 ). Indian hedgehog B function is required...

...Abed AlFatah Mansour; Sophie Khazanov-Zisman; Yaara Netser; Avihu Klar; Nissim Ben-Arie During embryogenesis, the dorsal roof plate and the ventral floor plate (FP) act as organizing centers to pattern the developing neural tube. Organizer-secreted morphogens provide signals that are interpreted...

...Emmanouil Metzakopian; Wei Lin; Mali Salmon-Divon; Heidi Dvinge; Elisabet Andersson; Johan Ericson; Thomas Perlmann; Jeffrey A. Whitsett; Paul Bertone; Siew-Lan Ang The transcription factors Foxa1 and Foxa2 promote the specification of midbrain dopaminergic (mDA) neurons and the floor plate...

... equally to this work Competing interests statement The authors declare no competing financial interests. 4 10 2011 © 2011. Smoothened V1 V2 Floor plate Gli3 Interneuron Zebrafish In mouse, Hedgehog (Hh) signalling is required for the formation...

... study shows that a subset of anterior floor plate-like cells gives rise to Fgf3 + SOX3 + progenitor cells, demonstrates a dual origin of infundibular cells and reveals a crucial role for FGF signalling in governing extended infundibular growth. FGF Floor plate Hypothalamus Chick...

... patterning, and describe a feedback circuit between the Shh pathway and homeodomain transcription factors that establishes non-graded regulation of Shh signaling activity. Nkx2 proteins intrinsically strengthen Shh responses in a feed-forward amplification and are required for ventral floor plate and p3...

...Keiji Inohaya; Yoshiro Takano; Akira Kudo The floor plate is a key organizer that controls the specification of neurons in the central nervous system. Here, we show a new role of the floor plate: segmental pattern formation of the vertebral column. Analysis of a spontaneous medaka mutant, fused...

...Shona D. Gray; J. Kim Dale Hensen's node of the chick embryo contains multipotent self-renewing progenitor cells that can contribute to either the floor plate or the notochord. Floor plate cells are a population of epithelial cells that lie at the ventral midline of the developing neural tube...

... trajectories parallel to the floor plate. To identify longitudinal guidance mechanisms in vivo, the overall role of floor plate tissue and the specific roles of Slit/Robo signals were tested. Ectopic induction or genetic deletion of the floor plate diverted longitudinal axons into abnormal trajectories...

... double- and Hes1;Hes3;Hes5 triple-null embryos( Fig. 6K,L ). In addition, we found that neurons (TuJ1-positive cells) are ectopically differentiated in the roof plate and the floor plate of Hes1;Hes3;Hes5 triple-null embryos(data not shown). Because cell death is not increased in Hes-null mice...

... views. (i-p) Lateral views with anterior towards the left and dorsal upwards. dl, dorsal lip; fp, floor plate; arc, archenteron; tel, telencephalon; ave, anterior ventral endoderm;ba, branchial arches; fnr, frontonasal region; age, presumptive anterior gut endoderm; nd, notochord; ov, otic vesicle. (C...

...Silvia L. López; María V. Rosato-Siri; Paula G. Franco; Alejandra R. Paganelli; Andrés E. Carrasco We have previously shown that the early Xenopus organiser contains cells equally potent to give rise to notochord or floor plate, and that Notch signalling triggers a binary decision, favouring...

...Iain Patten; Paul Kulesa; Michael M. Shen; Scott Fraser; Marysia Placzek To begin to reconcile models of floor plate formation in the vertebrate neural tube, we have performed experiments aimed at understanding the development of the early floor plate in the chick embryo. Using real-time analyses...

...Yongsu Jeong; Douglas J. Epstein The establishment of the floor plate at the ventral midline of the CNS is dependent on an inductive signaling process mediated by the secreted protein Sonic hedgehog (Shh). To understand molecularly how floor plate induction proceeds we identified a Shh-responsive...

...Jing Tian; Caleb Yam; Gayathri Balasundaram; Hui Wang; Aniket Gore; Karuna Sampath The floor plate, a specialized group of cells in the ventral midline of the neural tube of vertebrates, plays crucial roles in patterning the central nervous system. Recent work from zebrafish, chick, chick-quail...