... optical sections using the ImageJ Cell Counter plugin. Once acquired, the images were opened as stacks of optical planes and saved in .tiff format. For fate mapping injections in the pSHF, we used the DAPI and MF20 immunostaining channels to identify the different heart chambers at E9.5. Next, we...

... development. Using a Procr mGFP-2A- l acZ reporter, we reveal a much earlier Procr expression (embryonic day 7.5) than previously acknowledged (embryonic day 13.5). Genetic fate-mapping experiments using Procr Cre and Procr CreER demonstrate that Procr + cells give rise to blood vessels throughout the entire...

... behaviours that could be of clinical relevance. Cell tracking Developmental biology Fate mapping Light-sheet imaging Lineage tracing Live imaging Medical Research Council http://dx.doi.org/10.13039/501100007155 UK Research and Innovation MCUP1201/23 EMBO http...

... engineering Site-specific recombinase (SSR) Nigri-nox Fate mapping Cardiac valve development National Key Research and Development Program of China 2016YFC1300600 2017YFC1001303 2018YFA0107900 2018YFA0108100 Chinese Academy of Sciences 10.13039/501100002367 XDB19000000...

... regulatory state for pharyngeal muscle specification, whereas their regulatory relationships are more evolutionarily variable. Development Evolution Fate mapping Gene regulation Muscles The factors that specify myogenic potential upstream of the core MRF-based network in vertebrates differ...

... ( http://creativecommons.org/licenses/by/3.0 ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Clonal lineage CRE recombinase piggyBac transposase Multi-fluorescence Progenitor cells Fate mapping...

... cells. Fate-mapping experiments demonstrated that neighboring supporting cells acquired a hair cell fate, which increased in a basal to apical gradient, averaging over 120 regenerated hair cells per cochlea. The normally mitotically quiescent supporting cells proliferated after hair cell ablation...

... is spontaneously downregulated in NG2 cells in the normal embryonic ventral forebrain as they differentiate into astrocytes. To further examine the role of Olig2 in NG2 cell fate determination, we used genetic fate mapping of NG2 cells in constitutive and tamoxifen-inducible Olig2 conditional knockout mice...

... Cre/+ and Hoxb1 Cre/+ mice to label and fate map cranial NEC lineages, we have demonstrated that cells from the neural tube incorporate into the otic epithelium after otic placode induction has occurred. Pax3 Cre/+ labeled a more extensive population of NEC derivatives in the OV than did Wnt1-Cre...

..., which arise at mid-gestation as epithelial thickenings or placodes. However, the embryonic relationship between placodes, papillae and adult taste buds has not been defined. Here, using an inducible Cre-lox fate mapping approach with the Shh creER T2 mouse line, we demonstrate that Shh-expressing...

...-berlin.de ) * These authors contributed equally to this work † Present address: Bayer Schering Pharma AG, 13353 Berlin, German 6 11 2008 © 2009. 2009 Fate mapping Neuronal fate Transcription factor Mouse Brainstem neurons process and relay sensory information...

... gene as playing a novel, crucial role in thyroid development. han -expressing tissues surround the thyroid primordium throughout development. Fate mapping reveals that, even before the onset of thyroid-specific developmental gene expression, thyroid precursor cells are in close contact with han...

..., TN 37232-0225, USA 8 11 2006 © 2007. 2007 Notch Regulated intramembrane proteolysis (RIP) Cre recombinase Fate mapping Stem cells Mouse Notch signaling controls spatial patterning and cell fate decisions throughout the animal kingdom( Artavanis-Tsakonas et al.,1999...

... of expression of retinal markers. Fate mapping analysis using a multifunctional Chx10 BAC reporter mouse revealed this process to be direct transdifferentiation of retinal cells into pigmented cells. Microarray and in situ hybridization analyses revealed a complex program underlying the transdifferentiation...

..., the rhombomeres (r) 3 and 5. We now performed a detailed analysis of the fate of prospective r3 and r5 cells in Krox20 mutant embryos. Genetic fate mapping indicates that at least some of these cells persist in the absence of a functional Krox20 protein and uncovers the requirement for autoregulatory mechanisms...

[email protected] ) 05 03 2001 19 04 2001 © 2001 by Company of Biologists 2001 Muscle patterning Chick/quail chimera Somite Dermatome Confocal microscopy Fate mapping The first differentiated skeletal muscle cells to appear during early vertebrate development are those...

... 04 2001 © 2001 by Company of Biologists 2001 Muscle Morphogenesis DiI DiO Somite Involution Dermomyotome Confocal microscopy Fate mapping Chick During early avian embryogenesis, cells in the epiblast layer migrate through Hensen’s Node forming the endoderm...

.... and Stern , C. D. ( 1994 ). A fate map of the epiblast of the early chick embryo . Development 120 , 2879 – 2889 . 10.1242/dev.120.10.2879 Hayashi , K. and Ozawa , E. ( 1991 ). Vital labeling of somite-derived myogenic cells in the chicken limb bud . Roux’s Arch. Dev. Biol...

... (N.VII). 12, M. ceratoglossus (N.XII). 13, M. interceratobranchialis (N.XII/VII?). 14, M. branchimandibularis (N.IX/X). 15, M. cricohyoideus (N.IX/X/XII). Short survival fate mapping of NC populations emigrating from individual rhombomeres ( Lumsden et al., 1991 ) has shown...

... in the embryo. The small amount of mixing that does occur would result in variable locations of a small proportion of the descendants; this could contribute to the observed variability of the blastomere fate map. Because cell mixing during Xenopus development is insufficient to challenge possible lineage...