... can be used as a lineage tracing tool in mouse embryos. Fig. 1. TAT-Cre microinjection in E8.5 embryos recapitulates the fate map of posterior second heart field (pSHF) progenitors. (A) Experiment setup. (B) Posterior second heart field (pSHF) contribution ( n =10 embryos from two litters...

... development. Using a Procr mGFP-2A- l acZ reporter, we reveal a much earlier Procr expression (embryonic day 7.5) than previously acknowledged (embryonic day 13.5). Genetic fate-mapping experiments using Procr Cre and Procr CreER demonstrate that Procr + cells give rise to blood vessels throughout the entire...

... a compound photomicroscope equipped with Nomarksi/differential interference contrast in 1983 (adapted from Sulston et al., 1983 ) (B). (C) Post-implantation mouse embryonic fate maps were generated through years of observation, dye-labelling, grafting and electroporation experiments (adapted from Tam...

... engineering Site-specific recombinase (SSR) Nigri-nox Fate mapping Cardiac valve development Precise genetic manipulation of cells in vivo advances our understanding of organ development and tissue regeneration. The widely used DNA site-specific recombinase (SSR), Cre recombinase, allows...

.... Development Evolution Fate mapping Gene regulation Muscles In vertebrates, skeletal muscle development requires one or more of the four muscle regulatory factor (MRF)-family basic helix-loop-helix (bHLH) transcription factors, MyoD, Myf5, Mrf4 (also known as Myf6) and myogenin ( Block and Miller...

... ( http://creativecommons.org/licenses/by/3.0 ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Clonal lineage CRE recombinase piggyBac transposase Multi-fluorescence Progenitor cells Fate mapping...

... cells. Fate-mapping experiments demonstrated that neighboring supporting cells acquired a hair cell fate, which increased in a basal to apical gradient, averaging over 120 regenerated hair cells per cochlea. The normally mitotically quiescent supporting cells proliferated after hair cell ablation...

... is spontaneously downregulated in NG2 cells in the normal embryonic ventral forebrain as they differentiate into astrocytes. To further examine the role of Olig2 in NG2 cell fate determination, we used genetic fate mapping of NG2 cells in constitutive and tamoxifen-inducible Olig2 conditional knockout mice...

... Cre/+ and Hoxb1 Cre/+ mice to label and fate map cranial NEC lineages, we have demonstrated that cells from the neural tube incorporate into the otic epithelium after otic placode induction has occurred. Pax3 Cre/+ labeled a more extensive population of NEC derivatives in the OV than did Wnt1-Cre...

..., which arise at mid-gestation as epithelial thickenings or placodes. However, the embryonic relationship between placodes, papillae and adult taste buds has not been defined. Here, using an inducible Cre-lox fate mapping approach with the Shh creER T2 mouse line, we demonstrate that Shh-expressing...

...-berlin.de ) * These authors contributed equally to this work † Present address: Bayer Schering Pharma AG, 13353 Berlin, German 6 11 2008 © 2009. 2009 Fate mapping Neuronal fate Transcription factor Mouse Brainstem neurons process and relay sensory information...

... gene as playing a novel, crucial role in thyroid development. han -expressing tissues surround the thyroid primordium throughout development. Fate mapping reveals that, even before the onset of thyroid-specific developmental gene expression, thyroid precursor cells are in close contact with han...

..., TN 37232-0225, USA 8 11 2006 © 2007. 2007 Notch Regulated intramembrane proteolysis (RIP) Cre recombinase Fate mapping Stem cells Mouse Notch signaling controls spatial patterning and cell fate decisions throughout the animal kingdom( Artavanis-Tsakonas et al.,1999...

... of expression of retinal markers. Fate mapping analysis using a multifunctional Chx10 BAC reporter mouse revealed this process to be direct transdifferentiation of retinal cells into pigmented cells. Microarray and in situ hybridization analyses revealed a complex program underlying the transdifferentiation...

..., the rhombomeres (r) 3 and 5. We now performed a detailed analysis of the fate of prospective r3 and r5 cells in Krox20 mutant embryos. Genetic fate mapping indicates that at least some of these cells persist in the absence of a functional Krox20 protein and uncovers the requirement for autoregulatory mechanisms...

... for the formation of epaxial muscle during all of these phases is not available but primary myotome formation has been a focus of recent investigation employing cellular, molecular and experimental embryological strategies. Muscle patterning Chick/quail chimera Somite Dermatome Confocal microscopy Fate...

..., USA 05 03 2001 19 04 2001 © 2001 by Company of Biologists 2001 Muscle Morphogenesis DiI DiO Somite Involution Dermomyotome Confocal microscopy Fate mapping Chick During early avian embryogenesis, cells in the epiblast layer migrate through Hensen’s Node...

... factor chick fate mapping DiI DiO confocal microscopy The cellular mechanisms that generate skeletal muscle have been a subject of controversy for over a century. Fischel (1895) was the first to identify the dermomyotome epithelium of the somite as the source of myogenic progenitor cells...

... patterning fate mapping chick-quail chimeras rhombomeres neural crest segmentation vertebrate head evolution Patterning tasks for a developing vertebrate head include the definition of skeletal and muscular shapes and the establishment of a precise network of skeletomuscular connections...

... in the embryo. The small amount of mixing that does occur would result in variable locations of a small proportion of the descendants; this could contribute to the observed variability of the blastomere fate map. Because cell mixing during Xenopus development is insufficient to challenge possible lineage...