... 2021 Summary: This Review outlines genetically encoded biosensors that monitor ERK activity in living cells and discusses spatiotemporal ERK dynamics and their roles in developmental contexts. Biosensors ERK FRET KTR Optogenetics Japan Society for the Promotion of Science...

... in individual cells during this transition is unclear. Using a FRET-based biosensor, we monitored ERK signalling dynamics of single mouse ESCs during differentiation. ERK activity was highly heterogeneous, with considerable variability in ERK signalling between single cells within ESC colonies. Different...

... of development. Although individual components are known, the relationships among them remain unresolved. We now use FRET measurements of pathway activity, analysis of protein localization and genetic epistasis to dissect the structure of this network in Drosophila . We find that the adaptor protein Disabled...