... for live imaging, and that this protein can be used for quantitative applications, such as FRAP or laser ablation. Finally, we believe that this fluorescent protein, with the set of new possibilities it offers, constitutes an important tool for cell, developmental and mechano-biologists in their current...

... recovery after photobleaching (FRAP) in post-mitotic stages 7 to 9 of the Drosophila follicular epithelium ( Fig. S1 ). We first compared the cortical dynamics of Lgl with Scrib and Dlg using GFP-tagged proteins expressed at endogenous levels. Lgl is significantly more dynamic than Dlg and Scrib (Lgl t1...

... germline morphogens, but also somatic determinants. * Author for correspondence ( [email protected] ) Competing interests statement 18 5 2010 © 2010. C. elegans FCS FRAP MEX-5 Live-cell microscopy Protein diffusion A common mechanism of generating...

...Gabriella Ficz; Rainer Heintzmann; Donna J. Arndt-Jovin Fluorescence recovery after photobleaching (FRAP) microscopy was used to determine the kinetic properties of Polycomb group (PcG) proteins in whole living Drosophila organisms (embryos) and tissues (wing imaginal discs and salivary glands...