... role of this latter cell type in myoblast guidance. By creating a new Flk1/Pax3 double reporter mouse line, we have established the precise timetable for angioblast and myoblast delamination/migration from the somite to the limb bud. This timetable was conserved when mouse PSM was grafted...

... KDR VEGFR2 FLK1 Neuropilin Mouse Gonadotropin-releasing hormone (GnRH) neurons are neuroendocrine cells that are generated in the nasal placode from embryonic day (E) 10.5 onwards in mice, migrate along olfactory and vomeronasal nerves into the forebrain and then follow the caudal...

... tubulogenesis (alveolarization). Lungs with mis/overexpressed Wnt5a develop with severe pulmonary hypoplasia associated with altered expression patterns of Shh , L-CAM,fibronectin, VEGF and Flk1 . This hypoplastic phenotype is rescued by either replacement of the Shh protein or inhibition of fibronectin...

... © 2006. 2006 Mouse Embryo Hematopoiesis Vasculogenesis Angioblast Hemangioblast Flk1 Tie2 CD31 Oct3/4 Hematopoiesis in the mouse begins in the blood islands of the yolk sac (YS)at E7.5 ( Haar and Ackerman,1971 ; Moore and Metcalf,1970 ). Nucleated red blood cells in blood...

... that in the avascular chicken retina, vascular endothelial growth factor(VEGF) secreted by postmitotic neurons acts through the FLK1 receptor present on progenitor cells to influence cell proliferation and commitment. Augmenting VEGF signals increases progenitor cell proliferation and decreases retinal ganglion cell...

...Changwon Park; Iva Afrikanova; Yun Shin Chung; Wen Jie Zhang; Elizabeth Arentson; Guo hua Fong; Alexander Rosendahl; Kyunghee Choi The receptor tyrosine kinase FLK1 and the transcription factor SCL play crucial roles in the establishment of hematopoietic and endothelial cell lineages in mice. We...

... lineages, we have generated knock-in embryonic stem (ES) cells carrying a non-functional human CD4 at the Scl locus. By using in vitro differentiated Scl +/CD4 ES cells, we demonstrate that FLK1 and SCL are molecular determinants of the hemangioblast. Furthermore, our studies demonstrate that hematopoietic...

... functions downstream of cloche to regulate endothelial and blood differentiation. Ectopic expression of hhex through injection of a DNA construct leads to the premature and ectopic expression of early endothelial and blood differentiation genes such as fli1, flk1 and gata1 , indicating that Hhex can...

... and endothelial cells, the hemangioblast. Access to this initial cell population committed to the hematopoietic lineage provides a unique opportunity to characterize hematopoietic commitment events. Here, we show that BL-CFC expresses the receptor tyrosine kinase, Flk1, and thus we took advantage of the BL-CFC...

... mesoderm, me rely upon culturing in type IV collagen-coated dishes. Separation of the Flk1 + mesoderm f rom other cell lineages was critical for hemopoietic cell diffe rentiation, whe reas formation of the embryoid body was not. Since the two-dimensionally spreading cells can be monito red easily in real...