... images, which exhibited irregular fluorescent staining, low autofluorescence signal or heterogeneous follicles sizes. In the future, this pipeline will be useful for extensive cellular phenotyping in fish or mammals for developmental or toxicology studies. References Charleston , J. S...

...Stephan Kirchmaier; Burkhard Höckendorf; Eva Katharina Möller; Dorothee Bornhorst; Francois Spitz; Joachim Wittbrodt Established transgenesis methods for fish model systems allow efficient genomic integration of transgenes. However, thus far a way of controlling copy number and integration sites...

...Goro Yoshizaki; Masaki Ichikawa; Makoto Hayashi; Yoshiko Iwasaki; Misako Miwa; Shinya Shikina; Tomoyuki Okutsu The sexual plasticity of fish gonads declines after the sex differentiation period; however, information about the plasticity of the germ cells themselves after sex differentiation...

... of the medaka fish brain, we identified the simplet gene ( smp ), which is homologous to the human FAM53B gene. smp was expressed in actively proliferating cells of the CNS throughout embryogenesis. It belongs to a family of vertebrate-specific genes with no characterized biochemical domains. We showed...

... chromosome remains active until the blastocyst stage because of a rigid imprint that prevents inactivation, whereas the paternal X chromosome is preferentially inactivated in extra-embryonic tissues owing to lack of such imprint. In spite of random X-inactivation in XX androgenones, FISH analyses revealed...

... in vertebrate development. Here we report the isolation of Ol-eng2 , a medaka fish ( Oryzias latipes ) engrailed gene. We have monitored the effects of its widespread expression following mRNA injections in 1- and 2-cell medaka and Xenopus embryos. We found that the ectopic expression of Ol-eng2 predominantly...

... of Biologists 1998 Ultrabithorax abdominal-A Abdominal-B Trans -sensing Chromosome painting Homologous pairing CLSM FISH Pairing of homologous chromosomes in Diptera like Drosophila has been inferred from physical observations made in the early part of this century on the close...

...Thomas Schulte; Katja A. Paschke; Ute Laessing; Friedrich Lottspeich; Claudia A. O. Stuermer ABSTRACT Fish – in contrast to mammals – regenerate retinal ganglion cell axons when the optic nerve is severed. Optic nerve injury leads to reexpression of proteins, which typically are first expressed...

...Bahram Sadaghiani; Juergen R. Vielkind ABSTRACT We have studied neural crest development in two teleost fish species, Xiphophorus maculatus (platyfish) and X. heUeri (swordtail), and found similarities to that in other vertebrates but also some important differences. Unlike in other vertebrates...

..., secondary oogonia and early prophase oocytes showed no or only a slight cytoplasmic staining. Though female and male gonads were easily distinguishable from the age of 16 weeks onwards, it was observed in a few fishes (3 out of the 36 investigated specimens) that male germ cells were present...