... ( Conway and Schwartz, 2015 ) (labels EC junctions), Tg(kdr-l:ras-Cherry) s916 ( Hogan et al., 2009 ) (labels EC membrane), Tg(fli1ep:Lifeact-EGFP) ( Phng et al., 2013 ) (labels endothelial F-actin), Tg(fli1:NLS-mCherry) ( Heckel et al., 2015 ) (labels all EC nuclei) and Tg(ve-cad:ve-cadTS) ( Lagendijk et...

... cortex is mediated by F-actin. Specifically, an F-actin cage surrounds the microtubule spindle and applies forces to it. To better understand how F-actin transmits forces to the spindle, we studied a potential direct link between F-actin and microtubules. For this, we tested the implication of myosin-X...

... plasm RNPs and microtubule arrays. Calcium Germ plasm Ribonucleoparticles Microtubules F-actin Zebrafish National Institutes of Health 10.13039/100000002 F31GM108449 CA112369 GM065303 In the zebrafish Danio rerio , as in many animal species, primordial germ cell...

... through their association with the tips of growing interphase astral microtubules. Germ plasm RNPs are also associated with short cortical F-actin. We show that, in embryos mutant for the cytoskeletal regulator mid1ip1l , germ plasm RNPs fail to become recruited to the furrow, accumulating instead...

... domain, which emerges together with the basal and lateral domains. Drosophila Cortical domains Epithelial domains Epithelial polarity Subapical F-actin Small GTPase Guanyl nucleotide exchange factor Cellularization Ced-12 China Scholarship Council 10.13039/501100004543...

... from the prevailing view that Rho inhibits Hippo signaling through modulating cytoskeleton remodeling and/or cell polarity. Active Rho prevents the phosphorylation of Amot Ser176, thus stabilizing the interaction between Amot and F-actin, and restricting the binding between Amot and Nf2. Moreover, Rho...

..., and abnormal cell wound healing. We show that the cytokinesis defects are associated with aberrant cytoskeletal reorganization during furrow maturation, including abnormal F-actin enrichment and microtubule reorganization. Cortical F-actin prior to furrow formation fails to exhibit a normal transition into F...

... to a defect in F-actin organization, both in vitro and in vivo , which secondarily impairs EC adhesion and polarity. We also identify Cdc42 effectors Pak2/4 and N-WASP, as well as the actomyosin machinery, to be crucial for EC actin organization. This work supports the notion of Cdc42 as a central regulator...

... occurs in high cell density areas, conditional to F-actin distribution and polymerization. We propose that Yap is essential for fin regeneration and that its function is dependent on mechanical tension, conferred by a balancing act of cell density and cytoskeleton activity. * Author...

... was not required for adherens junction integrity, it was necessary for MZ localization of Moe, aPKC and F-actin. Furthermore, Moe and aPKC functioned antagonistically, suggesting that Moe limits Crb levels by reducing its interactions with the apical Par network. Additionally, Moe mutant cells lost Crb from...

... and through a Pak-independent non-canonical pathway to promote outgrowth. Whether this non-canonical pathway converges to promote Cofilin-dependent F-actin reorganization in axonal growth remains elusive. We demonstrate that Sickie, a homolog of the human microtubule-associated protein neuron navigator 2...

... of unbranched F-actin is induced by several members of the formin family. Drosophila encodes six formin genes, representing six of the seven known mammalian subclasses. Knittrig, the Drosophila homolog of mammalian FHOD1, is specifically expressed in the developing central nervous system midline glia...

..., we describe a cell-intrinsic pathway sculpting dendritic arborization (da) neurons in Drosophila that requires Longitudinals Lacking (Lola), a BTB/POZ transcription factor, and its control of the F-actin cytoskeleton through Spire (Spir), an actin nucleation protein. Loss of Lola from da neurons...

... the microvillar brush border of intestinal cells. Two of the isolated alleles ( kc2 and kc3 ) were mapped to the same gene, which we refer to as ifo-1 (intestinal filament organizer). The encoded polypeptide colocalizes with IF proteins and F-actin in the intestine. The apical localization of IFO-1 does not rely...

...-actin regulated Yap downstream of cell morphology. Cell morphology- and F-actin-regulated phosphorylation of Yap, and the effects of F-actin were suppressed by modulation of Lats. Our results suggest that cell morphology is an important factor in the regulation of the Hippo pathway, which is mediated...

... in the organization of the cytoplasmic F-actin meshwork during the first meiotic division. It is very dense in prophase I, undergoes a significant density drop upon meiosis resumption and reforms progressively later on. This meshwork remodeling correlates with endogenous formin 2 regulation. High formin 2 levels...

... impaired neural fold formation by attenuating F-actin accumulation and apical constriction, a cell-shape change that is required for neural tube folding. Conversely, the overexpression of nectin-2 in non-neural ectoderm induced ectopic apical constrictions with accumulated F-actin. However, experiments...

...Sven Bogdan; Oliver Grewe; Mareike Strunk; Alexandra Mertens; Christian Klämbt Regulation of growth cone and cell motility involves the coordinated control of F-actin dynamics. An important regulator of F-actin formation is the Arp2/3 complex, which in turn is activated by Wasp and Wave. A complex...

... 2003 © 2004. 2004 Moesin ERM Morphogenesis Photoreceptor Cytoskeleton F-actin Drosophila melanogaster ERM proteins, dynamic, regulated membrane-cytoskeleton linkers essential to apical membrane traffic and regulation( Bretscher et al., 2002 ; Reczek and Bretscher, 2001...

... that in vivo as well as in tissue culture models most of the Kette protein is found in the cytoplasm where it colocalizes with F-actin to which it can bind via its N-terminal domain. Some Kette protein is localized at the membrane and accumulates at focal contact sites. Loss of Kette protein results...