... that drive expression that approximates an aspect of native gene expression. Here, we explore how the Drosophila erecta even-skipped ( eve ) locus has evolved by testing its activity in the divergent D. melanogaster genome. We found, as has been reported previously, that the D. erecta eve stripe 2 enhancer...

... for the expression of the spider orthologues of even-skipped ( eve ) and runt-1 ( run-1 ), at least in part via caudal ( cad ). Moreover, it appears that dynamic expression of eve in this spider does not require a feedback loop with run-1 , as is found in the pair-rule circuit of the beetle Tribolium . Taken...

... work is properly attributed. Summary : Drosophila bicoid mutant embryos show severe patterning phenotypes, but individual cells retain wild-type fates - as judged by expression of key patterning genes at single-cell resolution. Canalization Drosophila Bicoid Even-skipped...

...) element at the 3′ border of the Drosophila even skipped ( eve ) locus. This element can maintain repression during embryonic as well as adult eye development. Transgenic dissection revealed that silencing activity depends on a binding site for the Polycomb-group protein Pleiohomeotic (Pho) and on pho gene...

... to specify final and unique neuronal cell fates are still unclear. In the Drosophila embryo, one well-studied motoneuron subclass, the intersegmental motor nerve (ISN), consists of seven unique motoneurons. Specification of the ISN subclass is dependent upon both even-skipped ( eve ) and the zfh1 zinc-finger...

... Krüppel Giant Even-skipped Dfd Scr Antp Ubx Short germ Long germ segmentation Gap gene Abdomen Jaws Tribolium castaneum Drosophila Evolution Parental RNAi Anteroposterior patterning in Drosophila is controlled by regulatory elements that measure the local...

...Paul Z. Liu; Thomas C. Kaufman The pair-rule gene even-skipped is required for the initiation of metameric pattern in Drosophila . But Drosophila segmentation is evolutionarily derived and is not representative of most insects. Therefore, in order to shed light on the evolution of insect...

... for correspondence (e-mail: arnosti@msu.edu ) 14 1 2004 © 2004. 2004 CtBP Knirps Even-skipped Enhancer Repression Transcription Drosophila Just as transcriptional activators are known to possess multiple activities to stimulate transcription, a growing number of transcriptional...

...Luiz Paulo Moura Andrioli; Vikram Vasisht; Ekaterina Theodosopoulou; Adam Oberstein; Stephen Small The striped expression pattern of the pair-rule gene even skipped ( eve ) is established by five stripe-specific enhancers, each of which responds in a unique way to gradients of positional...

...Mi Hye Song; Françoise Z. Huang; Gwendolen Y. Chang; David A. Weisblat We have identified homologs of the Drosophila pair-rule gene even-skipped in the glossiphoniid leeches Helobdella robusta and Theromyzon trizonare . In leech embryos, segments arise sequentially from five pairs of embryonic...

... , is expressed in the early mesoderm and later in the forming heart, suggesting a possible role in heart development. Here, we show that zfh-1 is specifically required for formation of the even-skipped ( eve )-expressing subset of pericardial cells (EPCs), without affecting the formation of their siblings...

...Miki Fujioka; Yukiko Emi-Sarker; Galina L. Yusibova; Tadaatsu Goto; James B. Jaynes ABSTRACT The entire functional even-skipped locus of Drosophila melanogaster is contained within a 16 kilobase region. As a transgene, this region is capable of rescuing even-skipped mutant flies to fertile...

.... Assaying expression of molecular markers in the developing nervous systems of various arthropods could resolve some of these issues. Here, we examine expression of Even-skipped and Engrailed, two transcription factors that serve as insect embryonic CNS markers, across a number of arthropod species...

...Michael Z. Ludwig; Nipam H. Patel; Martin Kreitman ABSTRACT Experimental investigations of eukaryotic enhancers suggest that multiple binding sites and trans -acting regulatory factors are often required for wild-type enhancer function. Genetic analysis of the stripe 2 enhancer of even-skipped...

... to this work 22 01 1998 25 11 1997 © 1998 by Company of Biologists 1998 Drosophila Germ cell migration Gonadal mesoderm abdominal A Abdominal B columbus even-skipped fear-of-intimacy fushi-tarazu heartless hedgehog huckebein odd-paired serpent...

... be defined by the overlapping expression of the head gap gene buttonhead ( btd ) and the primary pairrule gene even-skipped ( eve ). Re-examination of the btd and eve phenotypes in live embryos indicated that both genes are required for CF formation. Further, Eve expression in initiator cells was found...

... of ladybird causes a hyperplasia of heart precursors and alters the identity of even-skipped -positive pericardial cells. Loss of ladybird function leads to the opposite transformation, suggesting that ladybird participates in the determination of heart lineages and is required to specify the identities...

..., the stripe 2 enhancer of the pair-rule gene even-skipped ( eve ) was used to express kni in an ectopic position. Manipulating the stripe 2- kni expression constructs and examining transgenic lines with different insertion sites led to the establishment of a series of independent lines that displayed...

...Miki Fujioka; Pawel Miskiewicz; Lakshmi Raj; Alyssa A. Gulledge; Michael Weir; Tadaatsu Goto ABSTRACT The even-skipped ( eve ) pair-rule gene plays a key role in the establishment of the anterior-posterior segmental pattern of the Drosophila embryo. The continuously changing pattern of eve...

...David N. Arnosti; Scott Barolo; Michael Levine; Stephen Small ABSTRACT Previous studies have provided a detailed model for the regulation of even-skipped ( eve ) stripe 2 expression in the Drosophila embryo. The bicoid (bcd) regulatory gradient triggers the expression of hunchback ( hb...