... remain unclear. Here, we profiled spermatogonial epigenetic landscapes using surgically induced cryptorchid testis in the mouse. We show that cryptorchidism leads to alterations in local, but not global, H3K27me3 and H3K9me3 in undifferentiated spermatogonia. Of these, the loss of H3K27me3 was correlated...

... and epigenetics to establish cell state-specific gene expression patterns during lineage progression is a central aspect of cell differentiation, but the factors that regulate this elaborate interplay are not well-defined. The imprinted Dlk1-Dio3 noncoding RNA (ncRNA) cluster has been associated with metabolism...

... to reprogram somatic cells to induced pluripotent stem cells (iPSCs), mechanistic insights into the reprogramming process, and applications of iPSC technology in development and rejuvenation. Cell fate Epigenetics Induced pluripotent stem cells Reprogramming Small molecules Transcription factors...

... by chromosomal abnormalities, mutations and epigenetic defects. Epigenetic modifiers pre-program hundreds of spermatogenic genes in spermatogonial stem cells (SSCs) for expression later in spermatids, but it remains mostly unclear whether and how those genes are involved in fertility. Here, we report...

... defect . Nat. Genet. 37 , 423 - 428 . 10.1038/ng1526 Dror , E. , Fagnocchi , L. , Wegert , V. , Apostle , S. , Grimaldi , B. , Gruber , T. , Panzeri , I. , Heyne , S. , Höffler , K. D. , Kreiner , V. et al. ( 2023 ). Epigenetic dosage identifies...

... Rougeulle (Université Paris Cité/CNRS, Epigenetics and Cell Fate Center, Paris, France), Edda Schulz (Max Planck Institute for Molecular Genetics, Berlin, Germany) and James Turner (The Francis Crick Institute, London, UK). Originally scheduled for 2021, to commemorate the 60th anniversary of Mary Lyon's...

... Summary: This Review discusses the chromatin modifications associated with the mammalian female inactive X chromosome and their relevance to gene silencing and potential for therapeutic modulation. X inactivation Epigenetics Female Gene silencing Stem cells National Health and Medical...

... elements, that repetitive elements can function as a nuclear sink for epigenetic factors and that competition for H2A.Z greatly influences overall transcriptional output during development. These findings uncover general mechanisms in which counteractive biological processes underlie phenotypic outcomes...

... of existing sink occupation or by varying the size of the source, providing a central mechanism to explain a plethora of epigenetic phenomena, which contribute to phenotypic variegation, zygotic genome activation and nucleolar dominance. * Authors for correspondence ( [email protected]...

... it is not only an output, but also a driver of cellular functions, such as cell proliferation and maturation. Finally, we underscore the epigenetic and cellular mechanisms by which metabolic switches mediate biosynthetic needs during development and regeneration, and how understanding these mechanisms...

... metabolites that, in addition to serving as energy sources, contribute to epigenetic modifications. * Authors for correspondence ( [email protected] ; [email protected] ) Competing interests The authors declare no competing or financial interests. © 2023. Published by The Company...

... between histone modifications and developmental processes is challenging. In this Review, we discuss the use of dominant-negative histone mutations as tractable tools to study histone methylation in development. Chromatin Epigenetics Histone modification Stem cell Differentiation...

.... Maternal-fetal exchange Fetal development Programming Prenatal Extracellular vesicles Epigenetics A person's susceptibility to developing diseases such as diabetes and hypertension was previously thought to be determined by the interaction between genes and environment in adult life. However...

... , Z. , Dai , F. , Lu , C. and Tong , X. ( 2022 ). The Hox gene Antennapedia is essential for wing development in insects . Development 149 , dev199841 . 10.1242/dev.199841 Ferrell , J. E. , Jr. ( 2012 ). Bistability, bifurcations, and Waddington's epigenetic...

... pathways and a battery of transcription factors. However, the contribution of epigenetic cues to hypothalamus development remains unclear. We mutated the polycomb repressor complex 2 gene Eed in the developing mouse hypothalamus, which resulted in the loss of H3K27me3, a fundamental epigenetic repressor...

.... Summary: How the functional cooperativity of Tcf12 and NeuroD1 in specific subpopulations of the developing cortex creates the gene regulatory program essential for neuronal migration. Cortical development Neurogenesis Epigenetics Genomics Gene regulation Transcription factors...

... and epigenetic effects of pioneer-factor occupancy . Nat. Genet. 50 , 250 - 258 . 10.1038/s41588-017-0034-3 Doni Jayavelu , N. , Jajodia , A. , Mishra , A. and Hawkins , R. D. ( 2020 ). Candidate silencer elements for the human and mouse genomes . Nat. Commun. 11 , 1061...

.... Together, our results show that although Dppa2/4 are dispensable for ZGA transcription, maternal stores have an important role in offspring survival, potentially via epigenetic priming of developmental genes. To determine more globally whether there are any defects in ZGA, we performed single-embryo RNA...

... observational methods, whereas perturbational approaches have lacked precision. CRISPR-Cas9 has begun to transform the study of gene regulation, allowing for precise manipulation of genomic sequences, epigenetic functionalization and gene expression. CRISPR-Cas9 technology has already led to the discovery...

... Epigenetics Forebrain development Ribonucleoprotein complex Transcription factor binding Gene repression National Institutes of Health R35GM128938 R01MH111267 Ultraconserved elements (UCEs) were identified as 200 bp (or greater) segments of 100% DNA conservation between humans, mice...