... abnormalities, mutations, and epigenetic defects. Epigenetic modifiers pre-program hundreds of spermatogenic genes in spermatogonial stem cells (SSCs) for expression later in spermatids, but it remains mostly unclear whether and how those genes are involved in fertility. Here, we report that Wfdc15a , a WFDC...

... defect . Nat. Genet. 37 , 423 - 428 . 10.1038/ng1526 Dror , E. , Fagnocchi , L. , Wegert , V. , Apostle , S. , Grimaldi , B. , Gruber , T. , Panzeri , I. , Heyne , S. , Höffler , K. D. , Kreiner , V. et al. ( 2023 ). Epigenetic dosage identifies...

... Rougeulle (Université Paris Cité/CNRS, Epigenetics and Cell Fate Center, Paris, France), Edda Schulz (Max Planck Institute for Molecular Genetics, Berlin, Germany) and James Turner (The Francis Crick Institute, London, UK). Originally scheduled for 2021, to commemorate the 60th anniversary of Mary Lyon's...

... Summary: This Review discusses the chromatin modifications associated with the mammalian female inactive X chromosome and their relevance to gene silencing and potential for therapeutic modulation. X inactivation Epigenetics Female Gene silencing Stem cells National Health and Medical...

... elements, that repetitive elements can function as a nuclear sink for epigenetic factors and that competition for H2A.Z greatly influences overall transcriptional output during development. These findings uncover general mechanisms in which counteractive biological processes underlie phenotypic outcomes...

... of existing sink occupation or by varying the size of the source, providing a central mechanism to explain a plethora of epigenetic phenomena, which contribute to phenotypic variegation, zygotic genome activation and nucleolar dominance. * Authors for correspondence ( [email protected]...

... it is not only an output, but also a driver of cellular functions, such as cell proliferation and maturation. Finally, we underscore the epigenetic and cellular mechanisms by which metabolic switches mediate biosynthetic needs during development and regeneration, and how understanding these mechanisms...

... metabolites that, in addition to serving as energy sources, contribute to epigenetic modifications. The mechanistic requirement for pyruvate, besides energy production during ZGA, could be for histone acetylation, in which a negatively charged acetyl group is added to lysine residues on one end...

.... Chromatin Epigenetics Histone modification Stem cell Differentiation National Institutes of Health http://dx.doi.org/10.13039/100000002 R35GM142884 T32GM142607 R35GM142884-02S Boettcher Foundation http://dx.doi.org/10.13039/100005508 Histone modifications influence...

.... Maternal-fetal exchange Fetal development Programming Prenatal Extracellular vesicles Epigenetics A person's susceptibility to developing diseases such as diabetes and hypertension was previously thought to be determined by the interaction between genes and environment in adult life. However...

... of these developmental choices have remained hidden and have only been revealed through the use of simultaneous gene knockdowns and loss-of-function mutants. Support for this approach comes from other studies in which the simultaneous knockdown of multiple transcription factors and/or epigenetic regulators also revealed...

... pathways and a battery of transcription factors. However, the contribution of epigenetic cues to hypothalamus development remains unclear. We mutated the polycomb repressor complex 2 gene Eed in the developing mouse hypothalamus, which resulted in the loss of H3K27me3, a fundamental epigenetic repressor...

.... Summary: How the functional cooperativity of Tcf12 and NeuroD1 in specific subpopulations of the developing cortex creates the gene regulatory program essential for neuronal migration. Cortical development Neurogenesis Epigenetics Genomics Gene regulation Transcription factors...

... expression profiles during development. Chromatin Development Enhancers Global repression Epigenetics Pioneer factors Israel Science Foundation http://dx.doi.org/10.13039/501100003977 Rosetrees Trust http://dx.doi.org/10.13039/501100000833 Israel Cancer Research...

.... Together, our results show that although Dppa2/4 are dispensable for ZGA transcription, maternal stores have an important role in offspring survival, potentially via epigenetic priming of developmental genes. To determine more globally whether there are any defects in ZGA, we performed single-embryo RNA...

... observational methods, whereas perturbational approaches have lacked precision. CRISPR-Cas9 has begun to transform the study of gene regulation, allowing for precise manipulation of genomic sequences, epigenetic functionalization and gene expression. CRISPR-Cas9 technology has already led to the discovery...

... Epigenetics Forebrain development Ribonucleoprotein complex Transcription factor binding Gene repression National Institutes of Health R35GM128938 R01MH111267 Ultraconserved elements (UCEs) were identified as 200 bp (or greater) segments of 100% DNA conservation between humans, mice...

...Brandon S. Carpenter; Teresa W. Lee; Caroline F. Plott; Juan D. Rodriguez; Jovan S. Brockett; Dexter A. Myrick; David J. Katz ABSTRACT Formation of a zygote is coupled with extensive epigenetic reprogramming to enable appropriate inheritance of histone methylation and prevent developmental delays...

... SUV420H2 regulates embryonic stem (ES) cell fate by patterning the epigenetic landscape. Here, we report that depletion of SUV420H2 leads to a near-complete loss of H4K20me3 genome wide, dysregulated gene expression and delayed ES cell differentiation. SUV420H2-bound regions are enriched with repetitive...

... components of the H3K4 regulatory machinery mutated in neurodevelopmental disorders and the process of axon guidance in C. elegans . Thus, our study supports an epigenetic-based model for the aetiology of neurodevelopmental disorders, based on an aberrant axon guidance process originating from deregulated...